The expression of IGF-I and myostatin mRNAs in skeletal muscle of hypophysectomized and underfed rats during postnatal growth

Aim: To determine the roles of myostatin and insulin‐like growth factor‐I (IGF‐I) during postnatal growth, we examined IGF‐I and myostatin mRNA expression in the skeletal muscles of hypophysectomized and underfed rats during postnatal growth. Methods: Five‐week‐old rats were divided into four groups: freely fed control, moderately underfed, severely underfed and hypophysectomized. Four weeks later, blood and muscle samples were gathered to determine serum IGF‐I, myosin heavy chain (MHC) isoforms, IGF‐I Ea, IGF‐I Eb and myostatin mRNA. Results: The weights of soleus, plantaris and masseter muscles were decreased in underfed and hypophysectomized rats. Hypophysectomy resulted in significant increases of type I MHC at the expense of type IIx in plantaris muscle and of neonatal MHC at the expense of types IIx and IIb in masseter muscle. Serum IGF‐I was decreased by underfeeding and hypophysectomy. Plantaris muscle IGF‐I Ea mRNA in underfed and hypophysectomized rats was significantly lower than in normal controls. Plantaris muscle IGF‐I Eb mRNA in underfed rats was significantly lower than in normal controls. Masseter muscle IGF‐I Eb mRNA in severely underfed rats was significantly lower than in normal control and hypophysectomized rats. Soleus muscle myostatin mRNA in hypophysectomized rats was significantly higher than in normal and significantly underfed rats. No significant differences in plantaris and masseter muscle myostatin mRNA were observed between groups. Conclusion: Suppressed muscle growth caused by hypophysectomy and underfeeding may be attributed mainly to reduced circulating IGF‐I and partially to reduced IGF‐I mRNA, rather than to a change in myostatin.     

不同产地天花粉中重金属、砷盐的比较

目的对天花粉的安全性指标的制定提供依据。方法分析不同产地天花粉中重金属、砷盐。结果所有样品重金属含量均低于2×10一,有1个样品的砷盐含量大于5×10^-6结论建议天花粉的质量标准中增加砷盐检测项。     

初产妇不同会阴切口护理方法的效果观察

目的比较初产妇会阴切口不同护理方法的护理效果。方法把432例阴道自然分娩初产妇分娩顺序随机分为实验组、对照组,实验组采用0.5%碘伏擦洗联合清洁温水冲洗护理会阴切口;对照组采用0.5%碘伏擦洗护理会阴切口。比较两组会阴切口肿胀、疼痛、愈合情况。结果两组产妇产后48h会阴肿胀、疼痛没有显著性差异（P〉0.05）,在产后切口愈合情况存在明显差异（P〈0.5）。结论用0.5%碘伏擦洗联合清洁温水会阴冲洗,能有效促进会阴切口愈合,产妇感觉舒适。     

基于宫颈癌根治术后的尿潴留针灸与西药疗效对照研究

目的:对宫颈癌根治术后尿潴留的中西医治疗两种方法进行疗效比较研究。方法:将60例宫颈癌手术患者随机分为西药对照组和针灸治疗组,每组30例;术后7天,B超测量各组患者并残余尿量计算总有效率,并间接反映了重置尿管率。结果:针灸治疗组总有效率93.3%;重置尿管率6.7%;不良事件发生率0%;西药对照组总有效率66.7%;重置尿管率33.3%;不良事件发生率13.3%;两组总有效率并重置尿管率之间有统计学差别(P0.05)。西药对照组不良事件发生率大于针灸治疗组。结论:针灸治疗宫颈癌根治术后尿潴留较西药对照组疗效满意。     

不同产地寒水石中重金属及有害元素的含量研究

目的:建立寒水石中重金属及有害元素的测定方法,并对不同产地南、北寒水石中重金属及有害元素进行初步的比较研究。方法:样品经湿法消解后,采用原子荧光法测定寒水石样品中砷、汞;原子吸收分光光度法测定铅、镉、铜。同时对相应方法学进行考察。结果:寒水石中5种有害元素回收率在90%～104.5%,精密度<2%。铅、镉、铜、砷、汞的检出限分别为0.2、0.06、0.50、1.25×10-3、1.43×10-3μg.g-1。参照2010年版《中国药典》一部矿物药石膏项下标准,寒水石样品中仅砷含量有10.34%超标;参照2010版植物药标准,南寒水石汞含量3.45%超标,南、北寒水石镉含量13.79%超标;南、北寒水石重金属、有害元素分布没有明显规律。结论:本方法快捷、准确,灵敏度高,为进一步研究寒水石中有害元素成分及完善其质量控制标准提供可参考的依据。     

Biliary tract injury caused by different relative warm ischemia time in liver transplantation in rats

BACKGROUND:There is a controversy over the degree of liver and biliary injury caused by the period of secondary warm ischemia.A liver autotransplantation model was adopted because it excludes the effects of infection and immunological rejection on bile duct injury.This study was undertaken to assess biliary tract injury caused by relative warm ischemia(secondary warm ischemia time in the biliary tract)and reperfusion. METHODS:One hundred and two rats were randomly divided into 5 groups:groupⅠ(control);group...     

葡萄籽原花青素对人宫颈癌细胞的辐射增敏作用

目的:研究葡萄籽原花青素对人宫颈癌Hela细胞重离子辐射的增敏作用。方法:SRB法检测葡萄籽原花青素对人宫颈癌Hela细胞的增殖抑制作用,集落形成法检测其对重离子辐射的增敏作用,实时荧光定量检测葡萄籽原花青素对Hela细胞MRE-11基因mRNA的表达影响。结果:葡萄籽原花青素对人宫颈癌Hela细胞呈现剂量依赖性杀伤,但整体抑制率较低,IC50值为110.3μg/ml,单独应用抗肿瘤效果较弱;30μg/ml葡萄籽原花青素预处理后,人宫颈癌Hela细胞对重离子辐射的敏感性增加,增敏比为1.25;单独重离子辐射后,人宫颈癌Hela细胞Mre-11 mRNA表达显著上调,30μg/ml葡萄籽原花青素预处理可以降低由重离子辐射引起的Mre-11 mRNA表达的上调。结论:葡萄籽原花青素具有对人宫颈癌Hela细胞重离子辐射增敏作用,可能通过抑制Mre-11基因的表达而抑制DNA损伤修复。     

针灸药浴治疗慢性膝关节滑膜炎28例

[目的]观察针灸药浴治疗慢性膝关节滑膜炎的效果。[方法]根据辨症分析用针灸配合药浴治疗,20次后统计疗效。[结果]28例中,基本治愈18例,显效8例无效2例,有效率为92.9%。[结论]针灸药浴对慢性膝关节滑膜炎有较好的疗效。     

温针灸治疗子宫内膜异位症40例

目的:比较温针灸、普通针灸治疗子宫内膜异位症的临床疗效。方法:将80例患者随机分为温针灸治疗组和普通针刺对照组各40例。2组同取关元、中极、天枢、足三里、三阴交、太冲,普通针刺组不加温针灸。观察2组患者临床疗效和症状、体征方面的改变及不良反应。结果:治疗组的有效率为95.0%,对照组的有效率为77.5%(P<0.05);且治疗组在痛经、腰骶痛、肛门坠胀、月经不调、性交痛、不孕症等方面也明显优于对照组(P<0.05)。结论:温针灸是治疗子宫内膜异位症的有效方法之一。