Effects of Glucocorticoid on Electrical Activities of Hippocampal Neurons in vitro

Intracellular recording of the rat hippocampal slices was established and usedto analyse the effects of glucocorticoid on resting membrane potential as well as inputresistance of the cells in CA1 and CA2 of hippocampal slices.The results indicate thatwithin 1-2 min the steroid hyperpolarized some of the hippocampal neurons with atendency of increasing the cells' input resistance,suggesting that glucocorticoid caninfluence the function of central neurons in a non-genomic pattern,and the underlyingmechanism involves the change of resting membrane potential.     

Understanding the choroid

Based on previous work, this paper describes the anatomical and functional characteristics of the choroidal vasculature, namely the choriocapillaris, the arterial and arteriovenous anastomosis, the segmental distribution of the short posterior ciliary arteries, and the choroidal veins. The characteristics of the choroidal innervation with reference to the sensory nerve endings are reviewed. Some features of Bruch's membrane may explain the fundoscopic and angiographic appearance of the angioid streaks and the role of its basement membranes in the formation and transport of drusen.     

人血液酪氨酸蛋白激酶的研究——(Ⅲ)1—异丙基—6—氨基—噻吩骈[3,4—d]嘧啶—2,4—二酮对人淋巴细胞膜TPK活性的抑制作用

文报道了人工合成的1—异丙基—6—氨基—噻吩骈〔3,4—d〕嘧啶—2,4—二酮对人淋巴细胞膜TPK活性的抑制作用,IC_(50)约为300μmol/L。动力学实验结果表明,该化合物对TPK的抑制作用与ATP呈竞争性,而与多肽底物Poly(Glu—Ala—Tyr)_(6;3:1)呈非竞争性。放射自显影结果进一步证实了该化合物对TPK的抑制作用。     

脱钙冻干骨修复种植周骨缺损的扫描电镜观察

目的比较脱钙冻干骨(DFDB)、脱钙冻干骨复合人重组骨形成蛋白-2(rhBMP-2)、脱钙冻干骨联合钛膜的骨修复能力。方法 3条犬股骨种植体周形成4 mm×3 mm×3 mm的骨缺损,分别植入上述3种不同材料。术后4、8、12周分期处死动物,取含种植体的骨段进行扫描电镜观察,观察新骨形成情况及其与种植体之间的缝隙。结果 DFDB可单独用于修复种植体周骨缺损,但成骨作用较慢;DFDB+rhBMP-2和DFDB+钛膜能较早诱导新骨形成,加速骨整合过程。结论DFDB是一种较理想的骨修复材料,复合rhBMP-2或与钛膜联用时效果更佳。     

几丁糖联合聚乳酸薄膜预防硬膜外粘连的研究

目的 观察几丁糖加聚乳酸薄膜联合运用对椎板切除术后预防硬膜外瘢痕粘连的效果.方法 80只成年家兔[体质量(2.0±0.2)kg]制作椎板切除模型,在椎板缺损处分别覆盖等渗盐水(A组)、聚乳酸薄膜(B组)、几丁糖(C组)及几丁糖加聚乳酸薄膜(D组),术后12周对椎板切除部位进行大体观察、组织学观察及透射电镜比较各组间瘢痕形成和粘连情况.结果 B、C、D组的Rydell-Balazs粘连度评分、Nussvaum组织学评分均优于A组(P<0.01),D组优于B组和C组(P<0.01),B组与C组间差异无统计学意义(P>0.05).结论 联合应用几丁糖加聚乳酸薄膜能有效预防硬膜外瘢痕粘连,比单独应用几丁糖或聚乳酸薄膜效果好.     

Antibody Response Against the Glomerular Basement Membrane Protein Agrin in Patients with Transplant Glomerulopathy

Chronic allograft nephropathy (CAN) of renal allografts is still the most important cause of graft loss. A subset of these patients have transplant glomerulopathy (TGP), characterized by glomerular basement membrane (GBM) duplications, but of unknown etiology. Recently, a role for the immune system in the pathogenesis of TGP has been suggested. In 11 of 16 patients with TGP and in 3 of 16 controls with CAN in the absence of TGP we demonstrate circulating antibodies reactive with GBM isolates. The presence of anti-GBM antibodies was associated with the number of rejection episodes prior to diagnosis of TGP. Sera from the TGP patients also reacted with highly purified GBM heparan sulphate proteoglycans (HSPG). Indirect immunofluorescence with patient IgG showed a GBM-like staining pattern and colocalization with the HSPGs perlecan and especially agrin. Using patient IgG, we affinity purified the antigen and identified it as agrin. Reactivity with agrin was found in 7 of 16 (44%) of patients with TGP and in 7 of 11 (64%) patients with anti-GBM reactivity. In conclusion, we have identified a humoral response against the GBM-HSPG agrin in patients with TGP, which may play a role in the pathogenesis of TGP.     

Sub‐retinal neovascular membrane in exudative macular degeneration

Age‐related macular degeneration is a major cause of serious vision loss. The earliest stages of age‐related maculopathy may be defined by the size of the drusen present in the macula and the effects on vision. Further manifestations may include soft drusen, choroidal neovascularisation, macular haemorrhage and cicatricial or disciform degeneration of the macula. This report describes a patient with a macular haemorrhage, a choroidal neovascular membrane and serious loss of vision. In addition, the pathogenesis, diagnosis and treat‐ment options of macular degeneration are reviewed.     

板蓝根磷脂对内毒素血症小鼠巨噬细胞膜脂流动性的影响

目的　以小鼠内毒素血症为模型 ,观察板蓝根磷脂对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用。方法　小鼠分为板蓝根氯仿提取物预处理组、磷脂脂质体预处理组、板蓝根磷脂脂质体预处理组和内毒素血症对照组。各组按照上述次序分别给予腹腔注射 5ml/kg相应药物 ,预处理 18h后腹腔注射内毒素 6mg/kg。 6h后处死小鼠 ,观察细胞膜脂流动性的变化。结果　板蓝根氯仿提取物对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用没有达到具有统计学意义的程度 (P >0 .0 5 ) ,磷脂脂质体对膜脂流动性具有保护作用 (P <0 .0 5 ) ,但两者之间并没有统计学上的差别 (P >0 .0 5 ) ;板蓝根磷脂对内毒素血症小鼠细胞膜脂流动性具有明显的保护作用 (P <0 .0 1) ,优于单独使用板蓝根氯仿提取物 (P <0 .0 5 )或磷脂脂质体 (P =0 .0 5 )。结论　板蓝根磷脂脂质体对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用优于单独使用板蓝根氯仿提取物或磷脂脂质体     

Immunobead and Density Gradient Purification of Paget Cells: In vitro Studies of Proliferation

Previous studies using primary monolayer cultures of epithelial cells from the involved epidermis of patients with mammary and extramammary Paget's disease investigated whether Paget cells proliferate as other malignant cells do. Although epithelial monolayers from the involved skin were maintained for approximately 45 days, no permanent cell lines were established. The proportion of carcinoembryonic antigen (CEA)-positive cells did not increase in the long-term cultures. Herein, we report studies of whether there is a real reduction of Paget cell numbers or if this is merely a decrease in the expression of CEA by the cells. Furthermore, we investigated whether Paget cells survive longer when cultured free from any potential inhibitory keratinocytes or other epidermal cells. Skin samples were obtained from one patient with mammary Paget's disease and three with extramammary Paget's disease; epidermal cells were cultured in vitro. An enrichment of Paget cells was carried out from the cultured epidermal cells by combining an anti-epithelial membrane antigen monoclonal antibody, binding to immunobeads, and density gradient centrifugation in Nycodenz. The separated cells were re-cultured in Keratinocyte-SFM serum-free media. The proportion of CEA-positive cells did not increase in the cultures, and the purified cells did not show any increase in survival times compared to the non-purified cultured cells. These results suggest that the decrease of CEA-positive cells noted during culture results from a decline in expression of CEA in the Paget cells. Paget cells in the involved epidermis do not proliferate significantly and thus differ from many other malignant cells.     

液膜法提取硝酸水溶液中Ag~+的工艺过程

液膜的组成为:2%石油硫醚-4%多丁二酰亚胺-94%煤油,内相试剂为:1～2mol/L的氨水溶液。较为适宜的操作条件为:乳水比为1:3;内油比为0.8:1;外相硝酸浓度为0.5～1mol/L;常温操作。当料液中的银离子浓度小于800ppm时,一次提取率可达97%以上。本工艺具有分离速度快,效率高、选择性好等优点。