木黄酮对培养肝星状细胞增殖及脂质过氧化的影响

目的 以培养的大鼠肝星状细胞(HSC)为模型,测定植物雌激素木黄酮对HSC增殖及培养液中脂质过氧化产物的影响.以明确木黄酮抑制肝纤维化形成的作用.方法 采用培养的SD大鼠肝星状细胞(HSC-T6)为模型,培养后加入H2O3诱导氧化应激,分为3组,用不同浓度的木黄酮温育48 h,收集细胞培养基的上清液,每个浓度设6个重复组,用MTT法检测HSC增殖;收集细胞培养基上清液,采用试剂盒方法测定脂质过氧化产物超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱苷肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX).结果 木黄酮各剂量组不同程度降低HSC的增殖.呈剂量-效应关系.给予木黄酮后,MDA和GSH水平明显降低,SOD和GSH-PX活力明显升高.也呈剂量-效应关系.结论 植物雌激素木黄酮具有抑制肝纤维化形成的作用,其作用机制可能与抑制HSC的增殖及抗HSC氧化应激、抗脂质过氧化作用有关.     

Neurostimulatory and antioxidative effects of L-deprenyl in aged rat brain regions

This study investigated the influence of chronically administered L-deprenyl on normal ageing-related parameters: multiple unit action potentials, the activities of the enzymes Na⁺,K⁺-adenosinetriphosphatase, glutathione-s-transferase and glutathione peroxidase, and the levels of lipid peroxidation products and lipofuscin contents in the brain regions (cerebral cortex, hippocampus, striatum and thalamus) of 24-month-old rats. The drug increased the activity of Na⁺,K⁺-ATPase and glutathione-s-transferase. The activity of glutathione peroxidase remained unaffected. The drug also increased the multiple unit action potentials activity. The levels of lipid peroxidation products were, however, decreased, and lipofuscin accumulation was diminished by the drug. The results essentially indicated that chronic treatment of rats with L-deprenyl significantly influenced the ageing-related alterations in: multiple unit action potentials, Na⁺,K⁺-adenosinetriphosphatase, glutathione-s-transferase, lipid peroxidation products and lipofuscin accumulation. These novel data on the effect of L-deprenyl on parameters of normal ageing provide new additional evidence concerning the anti-ageing therapeutic potential of L-deprenyl. This revised version was published online in July 2006 with corrections to the Cover Date.     

脑梗死与心肌梗死患者血脂、血尿酸、纤维蛋白原和血小板参数的变化及其意义

目的探讨脑梗死与心肌梗死患者血脂、血尿酸、纤维蛋白原和血小板参数的变化及意义。方法将206例患者按病因分为心肌梗死组(69例)、脑梗死组(72例)和脑梗死合并心肌梗死组(65例);76例健康人为正常对照组。分别检测三酰甘油(TG)、总胆固醇(TCH)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白-A1(APOA1)、载脂蛋白-B(APOB)、血尿酸(UA)、纤维蛋白原(Fib)、血小板压积(PCT)、血小板平均体积(MPV)和血小板体积分布宽度(PDW)的水平。结果脑梗死组、心肌梗死组患者的UA、Fib水较正常对照组升高,差异有统计学意义(P<0.01,P<0.05);MPV、LDL-C水平较正常对照组升高(P<0.05),且脑梗死和心肌梗死对UA、Fib、MPV、LDL-C指标的改变无交互效应(P>0.05)。脑梗死组和心肌梗死组患者的PDW水平较正常对照组升高、PCT水平较正常对照组降低,差异有统计学意义(P<0.05);TG、TCH和APOB的水平较正常对照组升高,差异有统计学意义(P<0.05);HDL-C和APOA1水平较正常对照组降低,差异有统计学意义(P<0.01),且脑梗死和心肌梗死对PDW、PCT的改变有交互效应(P<0.01)、对TG、TCH、APOB、APOA1、HDL-C的改变有交互效应(P<0.05)。结论检测脑梗死与心肌梗死患者血脂、血尿酸、纤维蛋白原和血小板参数的变化,对判断病情及预后均有一定的意义。     

Effects of changes of membrane lipid microenvironment on CD71 protein conformation

Thecellmembraneisthebarrierbetweenitsinternalandexternalenvironments.Manystudieshavedemonstratedthattheconstituentsofmembranelipidsandthebiophysicalpropertiesofthemembranearecloselyrelatedtothefunctionalchangesofthecellsincetheycancontrolthepropertiesofcellmembrane,modulatethetransportsofcertainsubstances,thebindingofligandswithreceptors,thetransmissionoftransmembranoussignals,celldifferentiationandcellrecognition.Thefunctionalchangesofthecellinducedbytheconformationalchangesofmembranelipidsar…     

四妙方加味对2型糖尿病模型小鼠和慢性脂肪肝模型大鼠的影响

目的 :观察四妙方加味对2型糖尿病模型(T2DM)小鼠血糖、血脂及肝脏组织病理的影响及对慢性脂肪肝模型大鼠血脂、肝指数、Lee’s指数、脂肪指数的影响。方法:(1)通过腹腔注射链脲佐菌素(STZ)建立T2DM小鼠模型,选取成模小鼠随机分为6组:对照组,模型组,四妙方加味低、中、高剂量组和二甲双胍组,另设空白对照组。每天灌胃给予相应药物或蒸馏水,3周后测小鼠血糖及血清中总胆固醇(TC)、三酰甘油(TG)含量,计算肝重指数并观察小鼠肝组织病理变化。(2)灌胃给予高脂乳剂建立慢性脂肪肝大鼠模型,造模成功的大鼠随机分组5组,即模型组,阿托伐他汀组,四妙方低、中、高剂量组。另设空白对照组。各组口服给予相应的药物或蒸馏水。8周后取血测血清中TC、TG含量,称取大鼠脂肪的重量、肝脏重量,计算Lee’s指数和脂肪指数。结果:(1)与模型组比较,二甲双胍组和四妙方加味低、中、高剂量组小鼠血糖含量均显著下降(P<0.05或P<0.01);四妙方加味中、高剂量组TC含量明显低于模型组(P<0.05或P<0.01);四妙方加味各组TG含量均显著下降(P<0.05或P<0.01);二甲双胍组和四妙方加味比较,二者血糖、血脂、肝指数水平上无明显差异(P>0.05)。与模型组比较,四妙方加味中、高剂量组,均未发生脂肪变性和重度水样变性,脂肪变性指标差异不明显,水样变性指标有显著差异(P<0.01)。与二甲双胍相比,四妙方加味对肝组织的损伤有较强的缓解作用。(2)与对照组比较,四妙方加味中、高剂量组及阿托伐他汀组能降低血清中TC、TG含量(P<0.05或P<0.01)。四妙方加味低剂量组大鼠与模型组大鼠比较,脂肪指数降低(P<0.05);中、高剂量及阿托伐他汀组大鼠与模型组大鼠比较,肝指数、Lee’s指数、脂肪指数均降低(P<0.05或P<0.01)。四妙方加味组与阿托伐他汀组比较,差异无统计学意义(P>0.05)。结论:四妙方加味不仅具有显著的降糖作用,还能够降低血脂;且相较于二甲双胍,四妙方加味能抑制糖尿病小鼠肝脏肿大,减轻肝脏组织病理变化,对T2DM肝脏损伤具有一定的保护作用。     

Altered anti-oxidant status and increased lipid peroxidation in marasmic children

BACKGROUND: Protein energy malnutrition (PEM) is a common pediatric health problem in developing countries. Although the clinical features of PEM are well known, its pathophysiology is still unclear. Free radicals have been implicated in pathogenesis of PEM. In the present study, oxidant/anti-oxidant status in marasmus was investigated. METHODS: Red cell glutathione, glutathione peroxidase and superoxide dismutase and their related cofactors, serum selenium and copper, were studied in marasmic and control children. Serum lipid peroxidation was also evaluated to assess oxidative stress. RESULTS: The red cell glutathione levels and glutathione peroxidase activities were found to be significantly lower in the marasmic children than in the controls. Red cell superoxide dismutase (SOD) activity was not different between two groups. Serum selenium and copper concentrations were significantly lower in the marasmic children than in the control subjects. The malondialdehyde concentration, which is an index of lipid peroxidation, was significantly higher in the marasmic group compared with the controls. CONCLUSION: The anti-oxidant defense system was affected in marasmic children. Reduced anti-oxidant status and increased oxidative stress occurs in marasmic children.     

Lipid profiles in Polish adolescents from high- and low-risk families: tracking unfavourable lipid levels over a one-year period

In a country with a high cardiovascular mortality rate, lipid profiles were studied in 929 adolescents (440 from affected and 489 from non-affected families for cardiovascular disease and hypercholesterolaemia). In 334 children with elevated or borderline total cholesterol level, lipid profiles were re-measured after a 1-y period. In boys from affected families, in contrast to boys from non-affected families, significantly higher total cholesterol levels (4.36 +/- 0.81 vs 4.19 +/- 0.78 mmol/L, p < 0.05) and LDL-C level (2.1 +/- 0.72 vs 1.89 +/- 0.79 mmol/L, p < 0.05) and significantly lower HDL-cholesterol levels (1.81 +/- 0.34 vs 1.93 +/- 0.38 mmol/L, p < 0.05) were found. The odds ratio for being in the most unfavourable decile for LDL-cholesterol was significantly higher for girls from affected families (2.17, p = 0.02). A relatively high HDL-C level as well as a favourable TC/HDL-C ratio was demonstrated in all groups, being lowest in boys from affected families. A significant correlation was found between baseline lipids and their values re-measured after 1 y. It is concluded that (1) adolescents with a positive family history are at increased risk for unfavourable lipid profile, (2) adolescents with elevated total cholesterol and LDL-cholesterol levels remain hypercholesterolaemic after a 1-y period and are therefore candidates for further biochemical and clinical monitoring, and (3) children with elevated total cholesterol may not be at high risk for cardiovascular disease owing to the favourable TC/HDL-C ratio. The study results do not indicate that general cholesterol screening in Polish adolescents is necessary, as the proportion of children with elevated LDL-cholesterol is relatively low.     

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目的通过最新一轮的北京地区儿童青少年血脂调查,建立新的正常参考值。方法于2004-09,随机选取7~18岁北京市中小学生共971人,均来自北京市区及郊区各县。根据年龄(每3岁为一年龄段)及性别分为8组(7~9岁男、女组;~12岁男、女组;~15岁男、女组;~18岁男、女组),应用日立7060型全自动生化分析仪检测其空腹血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)浓度,分别取TC、LDL-C第75百分位及第90百分位点作为临界高限及高胆固醇血症浓度,取TG第90百分位点作为高甘油三酯血症浓度,取HDL-C第5百分位点作为低高密度脂蛋白浓度,建立北京地区儿童血脂参考值。结果971名儿童的血浆脂蛋白含量值呈正偏态分布,7~9岁男女性别组间血脂4项浓度;~12岁性别组间TG含量女高于男,余3项无统计学差异;~15岁性别组间TC及LDL-C含量女高于男,余2项无统计学差异;~18岁性别组间TC及HDL-C女高于男,余2项无统计学差异。结论通过对971名北京市中小学生的血脂调查,建立了北京地区不同年龄性别儿童青少年的血脂正常值,确立了临界高胆固醇和高胆固醇血症浓度、高甘油三酯血症浓度和低高密度脂蛋白浓度。     

Peroxidative stress and antioxidant enzymes in children with beta-thalassemia major

OBJECTIVE: Regular blood transfusions and secondary iron overload make thalassemic erythrocytes prone to peroxidative injury. Although some reports suggest endogenous free radical damage in thalassemia, there remains discrepancy in the status of antioxidant enzymes. The aim of this study was to evaluate the extent of lipid peroxidation and status of antioxidant enzyme in children with beta-thalassemia. METHODS: Fifty transfusion-dependent beta-thalassemics were subjected to analysis of lipoperoxides as malondialdehyde (MDA), nitric oxide (NOx), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GPx) along with serum iron and ferritin, liver functions and uric acid. Plasma MDA was analyzed to indicate the oxidative parameters, whereas the erythrocyte SOD, GPx, and plasma NOx were measured to show the antioxidant status of the children. All these parameters in 30 non-anemic healthy controls attending the child health promotion clinic of hospital were also studied. RESULTS: All the patients were iron overloaded. Markers of free radical injury such as MDA and antioxidant enzyme SOD and NOx levels were significantly elevated in thalassemic children while mean GPx levels were decreased in patients compared to controls (P < 0.001). All these markers significantly correlated with serum ferritin levels. There was no significant difference in levels of GSH measured but it correlated with serum iron levels. CONCLUSION: Our study results suggest that iron overload causes peroxidative damage in beta-thalassemia and antioxidant systems try to compensate for reducing lipid peroxidation to lower tissue damage.