单细胞凝胶电泳检测烹调油烟对淋巴细胞DNA的损伤作用

目的　研究烹调油烟对体外和体内小鼠淋巴细胞DNA损伤作用。方法　采用单细胞凝胶电泳 ,检测细胞DNA单链断裂 ,观察在不同剂量及浓度下的DNA损伤情况。结果　以 10、50、10 0及 2 0 0 μg/L的油烟颗粒有机提取物染毒小鼠淋巴细胞 ,各剂量组均可引起小鼠淋巴细胞DNA损伤。以 10、2 0、4 0mg/m3的烹调油烟染毒小鼠 ,也可引起小鼠体内淋巴细胞DNA损伤。烹调油烟引起的小鼠体外和体内淋巴细胞DNA损伤均存在剂量 -效应关系 ,且整体染毒显示存在时间 -效应关系。结论　一定剂量的烹调油烟能引起小鼠淋巴细胞DNA损伤     

Association of TERT gene polymorphisms with clinical benign prostatic hyperplasia in a Chinese Han population of the Northwest region

BackgroundTo investigate the association between single nucleotide polymorphisms (rs10078761, rs12696304, rs2853669, rs16847897, rs2736100, rs10069690) of telomerase gene (TERT) and the risk clinical benign prostatic hyperplasia (BPH) in a Chinese Han population of the Northwest region.MethodsA total of 150 BPH patients and 150 healthy older males from the northwest Chinese Han population were included in this study. The sample size for this unmatched case-control study was estimated by the look-up table method. Meanwhile, the general information and disease data of patients were collected. Age was only collected in healthy control subjects for statistical correction. Genotypes were detected using a multiplex PCR + ligase detection reaction (LDR). Typing results and clinical data were statistically analyzed using multiple linear regression and logistic regression. Pearson correlation was used for Hardy-Weinberg equilibrium.ResultsThe included population is in Hardy-Weinberg equilibrium. There was no significant association between SNP and the risk of BPH by correlation analysis. However, 4 haplotypes (TCTGGT, TCTGTC, TGCCTC, and TGTGTC) were identified as risk factors of BPH by haplotype analysis. The SNP rs2853669 is an independent risk factor for smooth muscle type of hyperplasia. Besides, rs2736100, rs10078761, and rs10069690 which are in linkage disequilibrium are associated with the severity of BPH.ConclusionsPolymorphism of the TERT gene determines the different disease development and pathological manifestations of BPH in the Chinese Han population the Northwest region.     

对单个活态细胞结构与功能多个水平层次的实时测定技术

本文介绍我们发展起来的．用于同时在胞内细胞分子水平、细胞骨架＼细胞膜、以及细胞整体形态三个水平层次上．对单个活态细胞的结构与功能进行无扰、实时、原位检测的系列技术及其有关应用。     

一片8Xc196Mc单片机的便携式多参数监护仪的研制

目的　研制一种便携式多参数生命指征监护仪。方法　以一片 8Xc1 96Mc单片机和一个高分辨率 (6 40× 2 0 0 )的液晶显示器 (LCD)为基础 ,采用信号检测和数据处理技术实现心电、血压、呼吸频率、体温 4个生理参数的实时检测和显示。结果　本系统可动态实时显示所采集的心电波形和其它的参数 ,以及所检测参数 2 4h的趋势图 ,并可通过RS2 32接口与PC机之间进行数据传输。结论　该仪器小型轻便 ,使用灵活 ,有较强的抗干扰能力 ,具有很大的应用价值     

食管癌中3p24和11p15.5染色体位点潜在抑癌基因的研究

目的：探讨３ｐ２４染色体位点的杂和缺失和１１ｐ１５．６位点的点突变同食管癌之间的相关性,寻找这２个位点中潜在的抑癌基因,并希望通过此研究能为最后确定该基因的功能奠定基础。方法：用ＰＣＲ－ＲＦＬＰ法检测３ｐ２４染色体的３个位点（ＥＡβＭＤ,ＥＡ,βＨ,ＥＡβＲ）上等位基因的杂合缺失情况,ＰＣＲ－ＳＳＣＰ法检测１１ｐ１５,位点的点突变。结果：经ＲＦＬＰ法显示：在３ｐ２４的ＥＡβＭＤ位点,杂合缺失率为７     

DSA单帧双期成像法在肝癌栓塞治疗中的应用

探讨肝癌栓塞治疗中 DSA成像的新方法。 1肝动脉加门静脉像 ,将 mask分别设在肝动脉早、中、晚期和实质期 ,对应的减影像设在门静脉期。 2肝动脉双期像 ,将 m ask设在肝动脉早期 ,对应的减影像设在肝动脉中、晚期和实质期。结果发现 :腹腔动脉 DSA单帧双期成像 37例次 ,肝动脉 DSA单帧双期重建 33例次。提示 :DSA单帧双期成像法有利于指导肝癌栓塞治疗的操作 ,提高超选择性肝内动脉插管成功率 ,缩短手术时间。     

硒对大鼠肝细胞DNA损伤的体内体外研究

应用单细胞凝胶电泳研究了一定剂量的亚硒酸钠对离体和在体大鼠肝细胞 DNA损伤的作用。结果表明 :在2 .185、 4.375、 8.75 0、 17.5 0 0、 35 .0 0 0 μmol/ L 的剂量条件下 ,除 2 .185 μmol/ L 的剂量外 ,其余剂量的亚硒酸钠均可引起离体大鼠肝细胞 DNA损伤。 5、 10、 2 0 μmol/ kg的亚硒酸钠也可引起在体大鼠肝细胞 DNA损伤。亚硒酸钠引起的离体和在体大鼠肝细胞 DNA损伤均存在明显的剂量 -反应关系。研究结果提示 ,一定剂量的亚硒酸钠能引起离体和在体大鼠肝细胞 DNA损伤     

抗前列腺特异抗原单链抗体/人羧肽酶A融合基因的构建及表达

目的 构建抗前列腺特异抗原（PSA）单链抗体（scFv)/人羧肽酶A(hCPA)融合基因,为前列腺癌的抗全导向酶-前体药物疗法奠定基础。方法 在已克隆抗PSAscFv和hCPA基因的基础上,用加端PCR分别在scFv基因的3′端和hCPA基因的5′端加上部分连接肽（linker)基因序列,回收后混合。应用重叠延伸拼接法,将抗PASscFv基因和hCPA基因通过linker序列串联为scFv/hCPA融合基因;并将构建的融合基因克隆到融合表达载体pGEX-4T-1中进行表达。结果 序列测定结果表明,构建的抗PSAscFv/hCPA融合基因中scFv和hCPA序列均正确,scFv和hCPA间有18bp的linker序列,推导的氨基酸序列为GSGGSG,与设计的序列相符,融合基因经IPTG诱导表达重组蛋白并以SDS-PAGE分析,在Mr为94000左右出现一条新生蛋白带,表达量约占菌体总蛋白的34%。结论 成功构建并原核表达了抗PSAscFv/hCPA融合基因。     

A Simple and Rapid Fluorometric Determination Method of α1-Acid Glycoprotein in Serum Using Quinaldine Red

We examined different fluorescent probes suitable for fluorometric determination of ₁-acid glycoprotein (AGP) in serum. Quinaldine red (QR) was shown to bind strongly and selectively to AGP. Taking advantage of the enhanced fluorescence of QR in the presence of AGP, we developed a direct method for the determination of serum AGP without removal of other serum proteins such as albumin. AGP concentrations in serum of healthy volunteers and patients correlated well with results from the conventional single radial immunodiffusion (SRID) method (r = 0.93, slope = 1). The newly developed method is faster and has a larger analytical concentration range than the SRID method. This method can also be used to determine AGP in serum of experimental animals, and it can serve to monitor AGP serum concentrations for pharmacokinetic evaluation of basic drugs.