用HPLC指纹图谱对复方甘草片实施全质量控制研究

目的采用双定性双定量相似度作为评价指标,建立了复方甘草片（CLTs）HPLC指纹图谱的全质量控制方法。方法采用RP-HPLC法以Century SIL C18BDS柱（200mm×4.6mm,5μm）;以色谱指纹图谱相对指数Fr为指标优化选择指纹图谱检测条件,确定流动相为1%醋酸水-1%醋酸乙腈低压梯度洗脱,紫外检测波长：254nm,柱温：（30.00±0.15）℃,进样量：5μL。以双定性相似度为参量通过对21批CLTs进行系统聚类分析确定用其中13批CLTs对照指纹图谱（RFP）,以此RFP为基础对总计26批CLTs采用双定性双定量相似度法进行质量评价。结果以甘草酸峰为参照物峰,确定35个指纹峰,建立了复方甘草片HPLC指纹图谱。用双定性双定量相似度法评价出11批质量完全合格,3批含量明显偏高,6批含量明显偏低,1批化学成分分布比例不合格,有劣质品（伪品）5批。结论所建立HPLC指纹图谱具有较好的精密度和重现性,适用于复方甘草片的质量控制。双定性双定量相似度法是宏观定性定量评价中药制剂质量的最合理、最客观的指纹图谱评价技术。     

Multi-level modelling of physical activity in nuclear families

Background: Few studies focus on the different dyadic relations among family members to study physical activity (PA) levels.

Aim: The aim was to investigate predictors and sources of variance of PA levels in nuclear families using multi-level modelling.

Subjects and methods: The sample consisted of 2661 Portuguese four-member nuclear families (10?644 subjects). PA was measured using a questionnaire and socioeconomic status (SES) was assessed by parental occupation. Height and weight were measured in children, while parents self-reported their values.

Results: The results showed that intra-generational similarities were higher than inter-generational, with correlation values of 0.26 and 0.10, respectively. SES was unrelated to any family members’ PA level. Being male (β?=?0.26, t?=?21.77), being older (β?=??0.36, t?=??4.73) and greater BMI for mothers (β?=?0.02, t?=?2.55) had effects on individuals’ PA.

Conclusion: These results suggest a strong dyadic resemblance in PA, showed different effects of gender, age and BMI on individuals’ PA and demonstrated that multi-level modelling is a useful strategy to study PA in families.     

Effect of gut microbial composition and diversity on major inhaled allergen sensitization and onset of allergic rhinitis

BackgroundDecreased gut microbiota diversity is associated with gut dysbiosis and causes various diseases, including allergic diseases. We investigated the relationship between gut microbial diversity and sensitization to major inhaled allergens. Furthermore, the relationship of allergic symptom onset with bacterial composition in sensitized individuals was investigated.MethodsThis study included 1092 local residents who had participated in the Iwaki Health Promotion Project in 2016. Blood samples were analyzed to ascertain specific IgE levels against major inhaled allergens (JCP, HD1, Grass-mix, Weed-mix). Nasal symptoms were estimated by questionnaires. Fecal samples were analyzed for bacterial 16S rRNA using next generation sequencing. The diversity index (α-diversity, β-diversity) and the composition of gut microbes in phylum/order levels were compared between patients sensitized or unsensitized to allergen, and symptomatic and asymptomatic groups.ResultsSome α-diversity metrics were significantly decreased in patients who were sensitized to any/all four allergens compared with the unsensitized group. β-diversity differed significantly between those unsensitized and sensitized to all allergens (aged 20–49 years), and between those unsensitized and sensitized to any/all four allergens (aged ≥50 years). The relative abundance of Bacteroidales was significantly lower in the unsensitized than in the sensitized group. The composition and diversity of gut microbiota were similar between the symptomatic and asymptomatic groups.ConclusionsOur results suggest that lack of diversity in gut microbiota has an effect on sensitization to allergens. Bacteroidales in order level may affect sensitization; however, the onset of allergy symptoms was not significantly associated with bacterial composition and diversity.