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41.
目的 :了解河南地区人群中D16S539、D7S82 0 、D13S317基因座的遗传多态性 ,获得群体遗传数据。方法 :对2 30份无血缘关系个体的抗凝血样提取DNA ,应用多位点复合PCR扩增技术结合聚丙烯酰胺凝胶电泳分型方法调查D16S539、D7S82 0 、D13S317基因座在河南地区群体中的基因型分布。结果 :D16S539基因座在河南地区人群中存在 9个等位基因 ,2 8种基因型 ;D7S82 0 基因座存在 9个等位基因 ,2 9种基因型 ;D13S317存在 8个等位基因 ,31种基因型。基因型频率分布符合Hardy Weinberg平衡。结论 :D16S539、D7S82 0 、D13S317基因座是进行人类群体遗传学和法医遗传学研究十分有用的遗传标记。 相似文献
42.
中国北方汉族、维吾尔族、哈萨克族DYS19基因座的遗传多态性 总被引:4,自引:0,他引:4
目的;研究DYS19基因座在中国北方汉族、维吾尔族、哈萨克族群体中的遗传多态性及其法医学应用。方法:应用聚合酶链反应后变性聚丙烯酰胺凝胶电泳分离扩增产物结合银染显带的方法,对101例北方汉族、56例维吾尔族、30例哈萨克族无关男性个体的DYS19基因座进行检测。结果:DYS19基因座在3个群体中共检出5种等位基因,基因频率分布范围分别为0.069-0.594,0.071-0.500,0.100-0.667;个人识别机率分别为0.600,0.675,0.491。χ^2检验表明等位基因分布具有明显的人群差异。家系调查符合单体父系遗传方式。结论:DYS19基因座个人识别机率高,属较高鉴别能力的遗传标记系统,且具有明显的人群分布差异,在法医学及人类遗传学研究中具有重要的应用价值。 相似文献
43.
Mark A. Oyer Adam I. Edelstein Nathan F. Arnett Kevin D. Hardt David W. Manning Michael D. Stover 《The Journal of arthroplasty》2021,36(4):1336-1341
BackgroundSeveral variables are known to correlate with the successful completion of short-stay total hip arthroplasty (THA) protocols. The role of psychological factors remains unclear. We investigated the interaction between patient-reported measures of psychological fitness and successful completion of a short-stay THA protocol.MethodsWe performed a prospective cohort study of patients undergoing elective anterior total hip arthroplasty enrolled in a short-stay protocol (success defined as LOS ≤1 midnight versus failed, LOS >1 midnight). Psychological fitness was measured using the Patient-Reported Outcomes Measurement Information System (PROMIS) domains for self-efficacy, depression, anxiety, emotional support, and the ability to participate in social roles. PROMIS scores, patient demographics, and surgical factors were assessed for a relationship with failure to complete short-stay protocol.ResultsPatients that failed to complete the short-stay protocol had higher mean pre-operative PROMIS depression scores (50.8 vs 47.1, P = .025) and anxiety scores (53.6 vs 49.2, P = .008) and higher postoperative PROMIS depression (48.19 vs 43.49, P = .003) and anxiety scores (51.7 vs 47.1, P = .01). Demographic and surgical variables did not correlate with the successful completion of the short-stay protocol. That seventy-six percent of the patients did not adhere to the short-stay protocol was due to the inability to complete a physical therapy standardized safety assessment.ConclusionHigher levels of preoperative and postoperative anxiety and depression in otherwise psychologically healthy patients, is associated with an increased risk of failure to complete a short-stay protocol following THA. Targeted interventions are needed to facilitate rapid recovery in patients with psychological barriers to early mobilization. 相似文献
44.
目的:探讨新型短节段内固定器治疗腰椎滑脱症的临床应用价值。方法:对18例腰椎滑脱的脊椎应用新型内固定器进行复位、固定,恢复脊椎的正常解剖结构,并自髂嵴后部取骨行横突间植骨融合。结果:术后随访12 ̄24个月,平均18个月,滑脱复位率达90%,4 ̄5月后达到骨性愈合,无螺钉松动,拨出,断裂及脊住骨滑脱现象。结论:新型短节段内固定器治疗腰椎滑脱症复位率高,固定螺钉松动,拨出,断裂及再滑脱等并发症少,疗效 相似文献
45.
STR-PCR方法检测贵州小型香猪遗传变异性的研究 总被引:5,自引:0,他引:5
目的 准确分析贵州小型香猪种群遗传变异及近交程度。方法 采用10对短串联重复序列(STR)引物,对贵州小型香猪个体进行PCR检测。结果 在检测到的88个有效等位基因型中,纯合基因型50个,占55.7%。个体平均杂合率为0.4081,位点平均杂合率为0.5562。结论 贵州小型香猪具有一定遗传稳定性,符合封闭群动物遗传学特征。 相似文献
46.
《Seminars in Pediatric Surgery》2022,31(3):151181
Advancements in donor management, organ preservation and operative techniques, as well as immunosuppressive therapies, have provided children with intestinal failure and its complications a chance not only for enteral autonomy but also long-term survival through intestinal transplantation (ITx). First described in the 1960’s, experience has grown in managing these complex patients both pre- and post-transplant. The goals of this review are to provide a brief history of intestinal transplantation and intestinal rehabilitation in pediatric patients, followed by focused discussions of the indications for ITx, induction and maintenance immunosuppression therapies, common post-operative complications, and outcomes/quality of life post-transplant. 相似文献
47.
Wilsondisease(WD)isanautosomalrecessivedisorderofcoppermetabolismwithaworldwidefrequencyofbetween1/5000and1/30000inlivebirths... 相似文献
48.
Sestili P Clementi E Guidarelli A Sciorati C Cantoni O 《The European journal of neuroscience》2000,12(1):145-154
A short-term exposure to tert-butylhydroperoxide (tB-OOH) promoted a concentration-dependent formation of DNA single-strand breaks in PC12 cells. These events were paralleled by an increase in the cytosolic concentration of Ca2+ that was in part cleared by the mitochondria. Unlike the extent of Ca2+ mobilization and/or mitochondrial Ca2+ clearance, the DNA strand scission evoked by the hydroperoxide was markedly reduced by the nitric oxide (NO) scavenger 2-phenyl-4,4,5,5-tetramethylimidazolin-1-oxyl-3-oxide (PTIO) or by the NO synthase inhibitor N-nitro-L-arginine methylester (L-NAME). Inhibitors of electron transport (rotenone and myxothiazol), ruthenium red (RR, a polycation which inhibits the calcium uniporter of mitochondria), or peroxynitrite scavengers (Trolox and L-methionine) were as effective as PTIO or L-NAME in inhibiting the DNA-damaging response mediated by tB-OOH. Rotenone, RR or peroxynitrite scavengers did not further reduce the residual DNA cleavage observed following treatment with tB-OOH in L-NAME-supplemented cells. Exogenous NO also increased the DNA damage caused by tB-OOH in L-NAME-supplemented cells and this response was blunted by RR or by inhibitors of electron transport but was insensitive to peroxynitrite scavengers. We conclude that both endogenous and exogenous NO enhance the DNA cleavage generated by tB-OOH in PC12 cells. However, only endogenous NO set the bases for an involvement of peroxynitrite in this DNA-damaging response. 相似文献
49.
50.
目的研究短干扰RNA(short interfering RNA,siRNA)的抗肿瘤作用.方法采用MTT法检测siR-NA的生长抑制作用,H&E染色法观察细胞形态变化,TUNEL标记法检测核DNA断裂,Western Blot法分析蛋白表达水平变化和流式细胞法检测细胞周期分布变化.结果siRNA-Bcl2,siRNA-MDM2,siRNA-CDK2和siRNA-HRas可以明显抑制人乳腺癌MCF-7等肿瘤细胞的生长;siRNA-MDM2和siRNA-Bcl2可以引起MCF-7细胞染色质凝集;siRNA-Bcl2,siRNA-MDM2,siRNA-CDK2和siRNA-HRas均可以明显降低靶基因的表达水平,同时诱导MCF-7细胞发生染色体DNA断裂;siRNA-MDM2还可以导致MCF-7细胞发生G1期阻滞.结论siRNA可以明显抑制肿瘤细胞的生长,降低靶基因的表达水平,并诱导肿瘤细胞凋亡和周期阻滞,提示siRNA可能发展成为一类高效特异的新型抗肿瘤药物. 相似文献