脑梗塞患者短时记忆的测定

用临床记忆量表测定了40例脑梗塞患者和37例健康人。结果表明脑梗塞患者有显著的短时记忆障碍,梗塞部位、面积、病程与记忆障碍有一定关系,无意义图形再认与右脑功能关系最密切,其他分测验两侧半球区别不显著。     

Screening and Quantification of Depression in Mild-to-Moderate Dementia Through the GDS Short Forms

Abstract

We evaluated the validity of the 1-, 4- and 15-item versions of the GDS in 51 patients affected by mild to moderate dementia, using the long form as standard. The following sensitivity and specificity values were obtained: 66% and 91% for GDS-1, 93% and 87% for GDS-4, 79% and 100% for GDS-15. GDS-15 also showed good agreement with GDS-30 in defining the severity of depression. These findings support the use of GDS-4 for the screening of depression and of GDS-15 for its severity assessment.     

Enterotrophic effects of glucagon-like peptide 2 are enhanced by neurotensin

Combination therapy with enterotrophic agents may be useful in patients with the short bowel syndrome. The gut hormones neurotensin (NT) and glucagon-hke peptide 2 (GLP-2) are potent enterotrophic fattors when administered alone; however, their combined effects are not known. Using a GLP-2-producing tumor (STC-1), we determined whether administration of NT enhances the effect of GLP-2 on intestinal growth. Athymic mice were injected with STC-1 cells (6 × 10⁶) subcutaneously. Twenty-three days after STC-1 implantation, mice received either NT (300 μg/kg or 600 μg/kg) or saline solution (control) subcutaneously three times a day for 6 days. Two groups of tumor-free mice received either saline or NT for 6 days. At sacrifice, jejunum and ileum were collected, weighed, and analyzed for DNA and protein content. In the jejunum, NT combined with GLP-2 (from STC-1) increased weight, protein content (markers of mucosal hypertrophy), and DNA content (a marker of mucosal hyperplasia), compared to either NT or GLP-2 alone. In the ileum, the combination of NT and GLP-2 significantly increased weight and/or protein content compared to NT or GLP-2 alone. Administration of NT enhances the enterotrophic effects of GLP-2, augmenting hypertrophy of the entire small bowel and hyperplasia of the jejunum. The combination of NT and GLP-2 may be useful to enhance intestinal growth in patients with the short bowel syndrome. Supported in part by National Institutes of Health grants PO1 DK356O8, ROI DK48345, ROI AG10885, and T32 DK07633. Presented in part at the Surgical Forum of the American College of Surgeons, Chicago, Ill., October 12–17, 1997; and at the Thirty-Ninth Annual Meeting of The Society for Surgery of the Alimentary Tract, New Orleans, La., May 17–20, 1998.     

以H835-50氨基酸分析仪测定肌氨酸

研究了用氨基酸分析仪测定肌氨酸含量时的最大吸收波长、最佳浓度等,验证了分析短程序的可靠性并用于实践,为高转化率合成Ｎ－酰基肌氨酸钠提供准确的测定结果     

MRI STIR序列对非神经根源性腰痛患者的诊断价值

【摘要】 目的：观察MRI检查中短T1反转回复（short T1 inversion-recovery,STIR）成像技术对于非神经根源性腰痛患者腰椎退变性病变的显示能力,评价其临床应用价值。方法：2010年9月～2011年6月对在我院就诊的有腰痛症状但无神经根放射痛症状的患者进行腰椎MRI扫描,共纳入130例患者,其中男89例,女41例,年龄17~81岁,平均48.3±16.0岁。病史均超过1年。MRI扫描序列包括：矢状面TSE-T1WI、TSE-T2WI、STIR序列,横断面TSE-T2WI序列。对STIR图像及TSE-T2WI图像上腰椎退变性病变进行观察,观察内容包括：L1～S1椎间盘退变情况、椎间盘突出/膨出的节段及程度、腰椎小关节骨质增生情况、棘间韧带水肿情况和腰背部皮下软组织水肿情况。分别对STIR序列与TSE-T2WI序列发现腰椎间盘退变、椎间盘突出/膨出、椎小关节病变、腰部软组织病变的能力进行比较。结果：130例非神经根源性腰痛患者中,STIR序列对于腰椎间盘退变、棘间韧带水肿及腰背部皮下软组织水肿的检出数分别为482个、118节和25例,TSE-T2WI序列检出311个、42节和8例,两序列检出率有显著性差异（P<0.05）;STIR与TSE-T2WI序列对于腰椎间盘突出/膨出和椎小关节骨质增生的检出数均为182个和71个,无差异。两序列均发现5例腰椎滑脱,19例腰椎管狭窄。结论：MRI STIR序列对非神经根源性腰痛患者腰椎间盘退变、棘间韧带水肿及腰背部皮下软组织水肿显示能力优于MRI T2WI。     

四配子异源嵌合体导致的真两性畸形机制研究

目的 :报告 1例四配子异源嵌合体导致的真两性畸形并讨论其发病机制。　方法 :对 1例外生殖器模糊的患者外周血的淋巴细胞、经培养的皮肤成纤维细胞、两种不同性腺组织的成纤维细胞进行染色体核型分析 ,同时用X和Y染色体探针进行双色荧光原位杂交 (FISH) ;对患者红细胞血型、人类白细胞抗原 (HLA)和 77个短重复序列 (STR)微卫星标记进行检测 ;对患者性腺的 2种不同组织进行组织病理学检查 ;同时对患者的父母进行红细胞血型、HLA和STR检测。　结果 :患者外周血淋巴细胞、皮肤成纤维细胞、呈白色和黄色性腺组织的成纤维细胞染色体核型均为 4 6 ,XX/ 4 6 ,XY ;FISH检测所有细胞都显示了XX或XY的杂交信号。 4 6 ,XY的核型在外周血淋巴细胞、皮肤成纤维细胞和白色性腺组织的成纤维细胞中占优势 ;4 6 ,XX的核型在黄色性腺组织的成纤维细胞中占优势。外周血淋巴细胞及 3种不同组织培养物的STR位点检测、ABO血型分析和HLA检测都显示有 2个不同的单倍体来自父亲 ,1个单倍体来自母亲。组织病理学检查患者同一性腺上有两种不同组织 ,呈白色的组织是睾丸 ,呈黄色的组织是卵巢。　结论 :性腺组织病理学检查、染色体核型分析、FISH是鉴定真两性畸形患者的有效方法 ,红细胞血型、HLA和STR可为鉴定四配子异源嵌合体提供?     

A prospective short-term outcome study of a short metaphyseal fitting total hip arthroplasty

The purpose of this study was to determine whether the short, metaphyseal fitting femoral stem would achieve stable fixation without diaphyseal fixation. A total of 126 patients (144 hips) were included in the study, and their mean age was 53.9 years (26-65 years). The mean duration of follow-up was 4.5 years (4-5 years). The predominant diagnosis was osteonecrosis of femoral head (88 of 144 hips, or 61%). The mean preoperative Harris hip score was 45 points, which improved to 96 points by the final follow-up. Western Ontario and McMaster Universities Osteoarthritis score and patient's activity score were improved substantially at the final follow-up. This short, metaphyseal fitting cementless femoral component achieved stable fixation without diaphyseal fixation, and there was minimal stress-shielding bone resorption in the calcar region.     

一期内固定结合椎体成形术治疗脊柱跳跃性骨折合并骨质疏松症

目的 评价后路一期内固定结合椎体成形术治疗脊柱跳跃性骨折临床效果.方法 对15例(33椎)脊柱跳跃性骨折患者行一期后路钉棒内固定结合椎休成形术.术前行MRI或CT检查,在骨折累及三柱节段或神经损伤处行内固定,视椎管占位情况决定是否减压,对椎体后壁完整椎体行椎体成形术.结果 患者均获随访,时间5~24个月.无感染,伤口均一期愈合;无内固定失败;无继发后凸畸形加重,无迟发神经损伤;有神经损伤的患者均不有同程度的恢复.结论 一期后路内固定结合椎体成形术是治疗脊柱跳跃性骨折合并骨质疏松症的安全有效的方法.