Resin glycosides from the leaves and stems of <Emphasis Type="Italic">Ipomoea digitata</Emphasis>

Alkaline hydrolysis of the ether-soluble resin glycoside (jalapin) fraction of the leaves and stems of Ipomoea digitata L. (Convolvulaceae) gave six organic acids, isobutyric, (S)-2-methylbutyric, tiglic, n-decanoic, n-dodecanoic, and cinnamic acids, and two glycosidic acids, quamoclinic acid A and operculinic acid A. Further, a new genuine resin glycoside, named digitatajalapin I, was isolated from the jalapin fraction, along with three known resin glycosides. Their structures have been determined on the basis of chemical and spectroscopic data.     

Effect of phenylethanoid glycosides and polysaccharides from the seed of Plantago asiatica L. on the maturation of murine bone marrow-derived dendritic cells

The seed of Plantago asiatica L. is one of the most popular folk herbal medicines used in China and other Asian countries. In this study, phenylethanoid glycosides and polysaccharides were isolated from the seed of P. asiatica L. by using phytochemical investigation methods. A screening model of immunological activity by using dendritic cells as target cells was established to investigate the effects of these compounds on the phenotypic and functional maturation of dendritic cells. Compared with untreated cells, dendritic cells treated with acteoside, isoacteoside or polysaccharides expressed higher level of class II MHC and costimulatory molecule CD86 (B7-2). Functional maturation was confirmed by decreased endocytosis and increased naïve T cell stimulatory activity of dendritic cells. These results showed that acteoside, isoacteoside and polysaccharides from the seed of P. asiatica L. had significant immunoenhancing activity by inducing the maturation of dendritic cells.     

New anthracene glycosides from <Emphasis Type="Italic">Rhodomyrtus tomentosa</Emphasis> stimulate osteoblastic differentiation of MC3T3-E1 cells

Two new anthracene glycosides (1, 2) were isolated from aerial parts of Rhodomyrtus tomentosa, along with three known compounds (3–5). The structures of two new compounds were established to be 4,8,9,10-tetrahydroxy-2,3,7-trimethoxyanthracene-6-O-β-D-glucopyranoside (1) and 2,4,7,8,9,10-hexahydroxy-3-methoxyanthracene-6-O-α-L-rhamnopyranoside (2) based on spectroscopic and chemical methods. Among them, compound 1, 2, and 5 significantly (P<0.05) increased the alkaline phosphatase activity, collagen synthesis, and mineralization of the nodules of MC3T3-E1 osteoblastic cells compared to those of the control, respectively.     

HPLC法测定胃灵冲剂中芍药苷和甘草苷的含量

目的 建立胃灵冲剂中芍药苷和甘草苷的含量测定方法.方法 采用HPLC法,色谱柱为Hypersil ODS2(5μm,4.6 mm ×200 mm)柱,流动相为乙腈-0.1％(体积分数)磷酸溶液(体积比14∶86),检测波长为237 nm,流速为1.0 mL·min-1.结果 芍药苷的线性范围为0.1140 ～0.570...     

花刺参中两个新的四环三萜化合物

目的从花刺参Stichopus variegatus中分离活性皂苷类成分。方法将花刺参体内得到的总皂苷溶于吡啶-2-二氧六环(1︰1)的混合液中,130℃加热2.5 h,应用多种色谱技术对回流产物进行分离纯化,得到2个三萜皂苷类化合物。结果应用现代光谱技术(2D-NMR和ESI-MS)和化学方法鉴定其结构,分别为3-O-[β-D-吡喃木糖(1→2)-4′-O-磺酸钠-β-D-吡喃木糖]-海参烷-9-烯-3β,12α,17α,25β-四醇,命名为花刺参苷A(variegatuside A,1);3-O-β-D-吡喃木糖-16β-乙酰氧基-海参烷-9-烯-22,25-环氧-3β,12α,17α-三醇,命名为花刺参苷B(variegatuside B,2)。结论化合物1和2均为新化合物。     

可疑翼手参皂苷ColochirosideA体外抑制多种瘤细胞株增殖的研究

目的：研究可疑翼手参体内三萜皂苷ColochirosideA（cA）的体外抗肿瘤活性。方法：用稻瘟霉分生孢子模型对CA进行生物活性初筛,分别用MTT、SRB法检测CA对5种体外培养肿瘤细胞（BEL-7402、HO-8910、MDA—MB-435、MDA—MB-468、A431）和2种体外培养人血管内皮细胞（HMEC、HUVEC）的增殖抑制活性。结果：cA对稻瘟霉显示较强的抑制活性,最小活性浓度（MMDC）〈7．9ug／ml;对5种体外培养人肿瘤细胞显示显著抑制增殖活性,IC50为1．75ug／ml-3．66ug／ml;对2种体外培养人血管内皮细胞均显示显著抑制增殖活性,IC50值分别为2．55ug／ml和5．63ug／ml。结论：CA体外对多种肿瘤细胞具有抑制活性,有进一步研究开发的前景。     

仿刺参水溶性海参皂苷的分离纯化及其抑瘤活性研究

为了获得具有显著抑瘤活性的水溶性海参皂苷样品, 利用大孔树脂柱、硅胶柱和葡聚糖凝胶柱对水溶性海参皂苷进行了分离纯化, 并对其体内外抑瘤活性和诱导肿瘤细胞凋亡进行了检测。研究结果显示, 大孔树脂柱的70%乙醇洗脱组分以及硅胶柱色谱的部分纯化组分 (pSC-2和pSC-3) 对HeLa细胞、A-549肺癌细胞、SGC-7901胃癌细胞和Bel-7402肝癌细胞均具有显著的抑瘤活性; 经过葡聚糖凝胶柱色谱, 获得了纯度均在99.6%以上、具有三萜类皂苷性质的SC-2和SC-3纯化样品, 发现SC-2纯化样品不仅能浓度和时间依赖性地抑制HeLa细胞的体外增殖, 还能剂量依赖性地抑制S180实体瘤的体内生长, 提高荷瘤小鼠的胸腺指数和脾指数。细胞凋亡检测结果显示, SC-2纯化样品可剂量依赖性地诱导HeLa细胞凋亡, SC-2纯化样品 (10和50 mg·L^-1) 处理12 h的HeLa细胞均出现了DNA片段化。可见, 水溶性海参皂苷SC-2纯化样品具有显著的抑瘤活性, 其机制可能是通过诱导肿瘤细胞发生凋亡来实现的。     

毛相思子中的异黄酮类成分

目的研究毛相思子全草的化学成分。方法用柱色谱法分离得到化学成分，用光谱法测定其结构。结果纯化出一个异黄酮苷和3个异黄酮成分，分别是8-methylretusin-7-O-β-D-glucopyranoside(Ⅳ)、retusin 8-methyl ether(Ⅱ)、4’，7，8-trimethoxyisoflavone(Ⅲ)和afrormosin(Ⅵ)。结论这4个成分均为首次从该植物中分得。     

远志总苷对快速老化模型（SAM—P／8）小鼠学习记忆能力的作用及其机理研究

目的探讨远志总苷对快速老化模型小鼠学习记忆能力的作用及其机制。方法以SAM—P／8小鼠为对象，给予远志总苷，连续灌胃30d，采用水迷宫方法逐日测定小鼠的学习记忆能力，用化学比色法测定脑组织总蛋白含量、总抗氧化能力和乙酰胆碱酯酶活性，用放免法测定血清中IL-2含量。结果远志总苷能明显提高SAM—P／8小鼠的学习记忆能力，缩短到达目的地的时间；显著提高脑组织总蛋白含量、总抗氧化能力、降低乙酰胆碱酯酶活性及血清中IL-2含量。结论远志总苷可提高SAM—P／8小鼠的学习记忆能力，其作用机理可能与降低脑组织氧化损伤有关。