牛右心室节制索及右束支的解剖观测

目的了解牛右心室节制索及右束支的形态特点及分布规律,为比较解剖学和兽医学积累解剖学资料。方法24例新鲜市售牛心,用大体解剖和墨汁灌注方法显示牛右心室传导系。肉眼观察右心室内各结构的形态和分布,用游标卡尺测量数据,SPSS统计软件处理相关数据。结果节制索多为扁圆柱状(90.5%),长(45.48±1.32)mm,长直径(3.78±1.54)mm,短直径(2.55±1.26)mm,右束支长(65.52±20.78)mm,直径(1.45±0.56)mm。发现1例双节制索。Purkinje纤维的分布第Ⅰ、Ⅱ象限最密集,第Ⅲ三象限较少分布,第Ⅳ象限分布最少。结论牛心节制索的形态与其他哺乳动物相似,其中有右束支穿行,其表面有分布不均的Purkinje纤维。     

Development of high throughput optical sensor array for on-line pH monitoring in micro-scale cell culture environment

On-line pH detection of cell culture environment is necessary in a bioprocess or tissue engineering. Devices by means of electrochemical mechanisms for this purpose have been reported to be less suitable compared with optical-based sensing principles. More recently, some non-invasive optical sensing systems have been proposed for online pH monitoring of cell culture environment. However, these devices are not for multi-target pH monitoring purpose, and are large in scale and thus not appropriate for the pH monitoring at a micro scale such as in microbioreactor or microfluidic-based cell culture platform. To tackle these issues, an optical fiber sensor array for on-line pH monitoring was proposed using microfluidic technology. The working principle is based on the optical absorption of phenol red normally contained in culture medium. Different from other device of the similar working principle, the proposed device requires less liquid volume (less than 0.8 μl), is non-invasive, and particularly can be configured as an array for high throughput pH monitoring. The present device has been optimized for the shape of detection chamber in a microfluidic chip with the aid of computational fluid dynamics (CFD) simulation, to avoid flow dead zone and thus to reduce the response time of detection. Both simulation and experimental results revealed that the design of oval detection chamber (axis, 1.5 and 2.0 mm) can considerably reduce the response time. Preliminary test has proved that the optical pH detection device is able to detect pH with average detection sensitivity of 0.83 V/pH in the pH range of 6.8–7.8, which is normally experienced in mammalian cell culture.     

Malignant fibrous histiocytoma of the sinonasal tract

Background

Sinonasal malignant fibrous histiocytoma (MFH) is rare.

Methods

Twenty‐five patients were registered with a diagnosis of sinonasal MFH at our hospital in the past 30 years. Clinical data were retrospectively reviewed.

Results

Eight tumors were primary MFH and 17 tumors were post‐irradiated MFH, located within the radiation field for previous nasopharyngeal carcinoma. Twenty‐one tumors originated from the maxillary sinus, 3 from the nasopharynx, and 1 from the nasal cavity. Twenty‐three patients underwent surgery but only 12 tumors were removed completely. The 5‐year overall and disease‐free survival rates were 25.1% and 21.5%, respectively. Multivariate analyses showed that previous radiation was the only adverse prognostic factor for disease‐free survival (p = .045). The 5‐year disease‐free survival rates of primary MFH and post‐irradiated MFH were 72.9% and 0%.

Conclusion

In this series, post‐irradiated MFH was more common than primary MFH. The prognosis of post‐irradiated MFH is poor, whereas primary MFH is fair. © 2008 Wiley Periodicals, Inc. Head Neck, 2009     

胎儿终丝形态学及光镜研究

目的 观察正常胎儿终丝形态及其结构,为研究脊髓栓系综合征患儿的终丝结构提供参照标准.方法 测量并切除10例新鲜流产胎儿终丝,取其纵、横切面行光镜观察.结果 终丝由1～5μm直径的弹簧样纵向胶原纤维束组成,纤维束呈层分布,每层之间有空隙;层内纤维束之间也有空隙.层间隙、束间隙及束内每根纤维问由横向精细网状纤维连结,也可见到胶原纤维束交叉连结各束间及层问纤维.弹性纤维分散在胶原纤维束内或集中排列在胶原纤维束间.间隙可见少量毛细血管.结论 终丝的结构及成分改变可能与脊髓栓系综合征的发病密切相关.     

模拟长期口腔环境下桩核结合力的研究

目的比较冷热循环前后预成桩与树脂核的结合力。方法采用玻璃纤维桩分别与Paracore、C learfill、Durafill和F2000复合体核材料结合。测试冷热循环前后桩核间结合力并对其进行比较。结果冷热循环前后,Paracore和C learfill与桩的结合力无变化,Durafill和F2000复合体在冷热循环后与桩的结合力变弱。结论为获得良好的远期修复效果,应选择强度高、流动性好的树脂材料成核。     

匹鲁卡品癫痫模型及戊四氮点燃模型新生鼠行为学和组织学的对比研究

目的 比较匹鲁卡品(毛果芸香碱)点燃新生鼠痫性发作模型及戊四氮点燃模型的行为学特征及组织学改变,为实验模型的选择奠定基础.方法 选择SD新生大鼠为研究对象,制作两种不同的新生鼠癫痫模型,观察新生鼠的行为学表现、不同时点Nissl染色等组织学改变.结果 两种模型的行为学特征有所不同,匹鲁卡品点燃模型中未见神经元丢失及苔藓出芽现象,戊四氮癫痫持续状态模型中无神经元丢失,但明显出现苔藓纤维出芽现象.结论 腹腔注射匹鲁卡品小鼠模型是一种理想的颞叶癫痫动物模型,苔藓纤维出芽可作为判断癫痫持续状态模型是否成功的形态学标准.     

缺血后处理对缺血／再灌注胃黏膜自由基损伤的影响

目的研究缺血后处理（ischemic post—conditioning,I—postC）对大鼠胃缺血／再灌注（gastric ischemia／reperfusion,GI／R）损伤的影响。方法健康SD大鼠18只,随机分为3组,即对照组、缺血／再灌注组、缺血后处理组,分别检测大鼠胃黏膜损伤指数（GMDI）及胃黏膜组织抑制羟自由基能力、黄嘌呤氧化酶（XOD）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量。结果缺血／再灌注可造成胃黏膜明显损伤;缺血后处理明显减轻GI／R损伤,并使胃黏膜组织抑制羟自由基能力、SOD含量增加,XOD、MDA明显降低。结论缺血后处理减轻大鼠胃缺血／再灌注损伤,其保护机制与减轻缺血／再灌注胃黏膜自由基损伤有关。     

三种桩核冠修复在牙体缺损治疗的应用比较

目的:比较铸造合金桩核、钛及钛合金成品根管桩和弹性模量与牙本质相近的纤维桩制作桩核,3种方法修复残冠残根的临床效果。方法:牙体缺损患者共1368例,前牙牙体缺损776例。后牙牙体缺损592例。1368例患者分为3组,A组838例(铸造桩核),B组428例(钛及钛合金成品根管桩),C组102例(玻璃纤维桩),对3组的治疗效果进行比较。结果:随访0.5～3年,A组成功789例,失败49例;B组成功423例,失败5例;C组成功102例,无失败病例。结论:弹性模量与牙本质相近的纤维桩可减少根折的发生,修复疗效最好。钛及钛合金根管桩具有优良的生物相容性、耐腐蚀性,弹性模量相对较小,抗折能力强;有相对的优越性。铸造桩核的缺点是腐蚀、过敏使牙龈出现金属线牙龈变色,易出现根折。     

Phrenic nerve diabetic neuropathy in rats: unmyelinated fibers morphometry

We have demonstrated that phrenic nerves' large myelinated fibers in streptozotocin (STZ)-induced diabetic rats show axonal atrophy, which is reversed by insulin treatment. However, studies on structural abnormalities of the small myelinated and the unmyelinated fibers in the STZ-model of neuropathy are limited. Also, structural changes in the endoneural vasculature are not clearly described in this model and require detailed study. We have undertaken morphometric studies of the phrenic nerve in insulin-treated and untreated STZ-diabetic rats and non-diabetic control animals over a 12-week period. The presence of neuropathy was assessed by means of transmission electron microscopy, and morphometry of the unmyelinated fibers was performed. The most striking finding was the morphological evidence of small myelinated fiber neuropathy due to the STZ injection, which was not protected or reversed by conventional insulin treatment. This neuropathy was clearly associated with severe damage of the endoneural vessels present on both STZ groups, besides the insulin treatment. The STZ-diabetes model is widely used to investigate experimental diabetic neuropathies, but few studies have performed a detailed assessment of either unmyelinated fibers or capillary morphology in this animal model. The present study adds useful information for further investigations on the ultrastructural basis of nerve function in diabetes.