D16S539等5个短串联重复序列基因座遗传多态性分析

目的　探讨河南汉族群体 D16S53 9、D7S82 0、D13 S3 17、D8S1179和 D2 1S11等 5个基因座基因频率 ,获得群体遗传学数据。方法　随机抽取 2 0 9名河南汉族群体无血缘关系个体的静脉血 ,柠檬酸抗凝剂抗凝 ,酚 -氯仿法提取 DNA ,应用 PCR技术扩增上述 5个 STR基因座 ,聚丙烯酰胺凝胶垂直板电泳分型。结果　 2 0 9名个体中 D16S53 9、D7S82 0、D13 S3 17、D8S1179和 D2 1S11等 5个基因座分别检出 7、8、8、10、15个等位基因 ,基因型分布符合 Hardy-Weinberg平衡。 5个基因座的杂合度分别为 0 .790 7、0 .80 56、0 .7914、0 .82 3 9、0 .80 46,多态信息含量分别为 0 .7712、0 .780 1、0 .7694、0 .80 0 9、0 .7816,个人识别能力在 0 .9416、0 .93 67、0 .9511、0 .9546、0 .93 0 8,非父排除概率分别为 0 .610 6、0 .6179、0 .614 5、0 .70 2 2、0 .6712。结论　 5个 STR基因座具有高度多态性遗传标记特征 ,在群体遗传学研究和法医学应用中具有较高的价值     

乙型肝炎患者血清中分泌型IgA水平与HBV DNA含量的关系

湖北省鹤峰县人民医院检验科,鹤峰 445800目的 研究乙型肝炎患者血清中分泌型IgA(SIgA)水平与乙型肝炎病毒(HBV)DNA含量的关系,为临床提供一个新的评价HBV复制状态及肝细胞损伤的指标。方法 100份经ELISA检测并确诊为乙型肝炎的患者血清用荧光定量PCR检测HBV DNA的拷贝数,用ELISA并经酶标仪定量其SIgA的量。结果 SIgA的含量与HBVDNA的拷贝数的对数呈正相关(r=0.69,P<0.01),在HBsAg( )/HBeAg( )/HBcAb( )组和HBsAg( )/HBeAb( )/HBcAb( )组中SIgA含量差异无显著性意义(P>0.05),而HBV DNA拷贝数前者明显高于后者(P<0.01)。结论 乙型肝炎患者血清SIgA的含量在评价HBV的传染性及肝细胞损伤程度方面比乙型肝炎血清学标志物(HBV-M)更灵敏、准确。     

Expression of myosin heavy chain isoforms in Duchenne muscular dystrophy patients and carriers

The expression of MHC isoforms in the skeletal muscles of nine patients with Duchenne muscular dystrophy (DMD) (from 2.5 to 15 yr of age) and three DMD carriers was studied using different specific anti-MHC MAbs. We also analyzed muscle fiber size and fiber reactivity with acridine orange and/or with a surface antigen marker. One-quarter of all fibers of DMD patients, or less with age, were of normal size and contained only adult slow MHC. Half of the muscle fibers contained adult and developmental MHCs. Only half of these fibers were representative of an active regenerative process. MHC co-expression also altered the proportion of normal fast or slow fibers. Adult fast MHCs were expressed as unique MHC only in small and very small fibers in the oldest DMD patients. In DMD carrier muscles, the greatest alterations in MHC expression were observed in patients with the most reduced dystrophin expression. However, MHC changes in dystrophin-positive fibers were similar to those observed in dystrophin-free fibers. In conclusion, disruptions or delays in the switching of all genes coding for adult fast and slow MHC and developmental MHC coincided with dystrophin deletion and with perturbations in its expression.     

抗结核药物不良反应293例临床分析

[背景]观察抗结核药物的不良反应.[病例报告]对293例应用抗结核药物后出现不良反应的患者资料进行分析,抗结核药物常见不良反应的有肝脏损害、胃肠道反应、听力障碍及关节痛等.[讨论]应依据患者的具体情况,选择不同的抗结核药物进行联合应用.     

Up-regulation of urinary UPIII mRNA levels in vesicoureteral reflux patients: Potential application as a screening test for vesicoureteral reflux

OBJECTIVES: Vesicoureteral reflux (VUR) is the most common congenital urinary tract anomaly. This disease can pose a major threat to the kidneys as twenty percent of patients with endstage renal disease are reported to have VUR. Although genetic studies for uroplakin III (UPIII) have been reported recently, no study has focused on UPIII gene expression in VUR patients. We describe here the up-regulation of UPIII mRNA in exfoliated urinary cells from primary VUR patients. METHODS: A real-time RT-PCR for UPIII mRNA was performed on exfoliated urothelial cells from 18 primary VUR and 38 control samples. UPIII mRNA copies were calculated for each sample. The statistical differences were assessed by the Mann-Whitney U test. Receiver operator characteristic curves were constructed for analysis of the diagnostic values. RESULTS: UPIII mRNA was found to be up-regulated to a greater extent in VUR than in control exfoliated urinary cells (mean +/- SE: 497.0 +/- 178.5 copies vs. 69.0 +/- 10.0 copies, respectively, P < 0.001). In evaluating the measurement of urinary UPIII mRNA as a screening test for VUR, the sensitivity was 77.8% and the specificity was 76.3% by the best diagnostic cutoff point. CONCLUSIONS: This is the first report demonstrating up-regulation of UPIII in mRNA levels in VUR patients. We submit that the quantitative measurement of urinary UPIII mRNA has a potential of developing into the first non-invasive screening test for VUR.     

间接免疫荧光法检测婴幼儿急性下呼吸道合胞病毒感染的特异性IgM

本文对1984年1～3月和1985年3～8月共107例急性下呼吸道感染的住院患儿,采用间接免疫荧光法检测其急性期血清抗RSV特异性IgM抗体,并与病毒分离和/或中和试验比较,敏感性为82.1％,特异性为71.8％。RSV感染患儿发病后3天内大多数病例即可从血清中检测出RSV-IgM,因此该法具有早期诊断价值。     

The interactive role of mucosal T lymphocytes in intestinal growth, development and enteropathy

Abstract Over the past 15–20 years, research has progressively focused on the mucosal T cell as the central factor in the initiation of physiological or pathological changes, first in the growth and maturation of the early (postnatal) intestine, and second in adult-type enteropathies resulting from sensitivity to either food or pathogen-derived antigens. T cell-mediated events may be measured, for example, in terms of specific immunopathologic patterns of change and injury, such as type 1 (lymphocyte infiltration), type 2 (crypt hyperplasia) and type 3 (flat-destructive), which can be recognized and quantitated microscopically; by determination of lymphocyte reactivity through secretion of interleukin-2 receptors (IL-2R) into plasma or expression by mucosal lymphocytes; by quantitation of lymphocyte subsets emigrating into inflamed tissues by immunoperoxidase-labelled monoclonal antibodies; or by the determination of T cell receptor polymorphisms. Alterations in intestinal growth, structure and function at weaning are likely to be T cell-mediated as they are analogous to the same type 1/2 lesions that reflect modulation of adult mucosal architecture in food and parasite-induced hypersensitivity reactions. Enteropathies associated with HIV infection and T cell deficiency display a milder degree of villous flattening and impaired crypt hyperplasia than that typical of gluten-sensitivity, suggesting a reversion to lesser degrees of mucosal pathology (type 1/2). Clearly more information will accrue; meanwhile the remarks in this brief survey should provide a firm basis whereby clinician and scientist can meet, and together recognize and further dissect the modulatory effect of T lymphocytes on mucosal structure and function.