全文获取类型
收费全文 | 350篇 |
免费 | 18篇 |
国内免费 | 9篇 |
专业分类
妇产科学 | 38篇 |
基础医学 | 59篇 |
口腔科学 | 3篇 |
临床医学 | 5篇 |
内科学 | 40篇 |
皮肤病学 | 1篇 |
神经病学 | 12篇 |
特种医学 | 16篇 |
外科学 | 20篇 |
综合类 | 36篇 |
预防医学 | 30篇 |
眼科学 | 37篇 |
药学 | 50篇 |
中国医学 | 27篇 |
肿瘤学 | 3篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 9篇 |
2021年 | 18篇 |
2020年 | 8篇 |
2019年 | 9篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 2篇 |
2015年 | 8篇 |
2014年 | 25篇 |
2013年 | 25篇 |
2012年 | 26篇 |
2011年 | 23篇 |
2010年 | 23篇 |
2009年 | 14篇 |
2008年 | 13篇 |
2007年 | 5篇 |
2006年 | 15篇 |
2005年 | 7篇 |
2004年 | 19篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 18篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 4篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1971年 | 1篇 |
排序方式: 共有377条查询结果,搜索用时 93 毫秒
151.
Accuracy of Preimplantation Diagnosis of Single-Gene Disorders by Polar Body Analysis of Oocytes 总被引:8,自引:0,他引:8
Rechitsky S Strom C Verlinsky O Amet T Ivakhnenko V Kukharenko V Kuliev A Verlinsky Y 《Journal of assisted reproduction and genetics》1999,16(4):192-198
Purpose:
A number of pitfalls in single-cell DNA analysis, including undetected DNA contamination, undetected allele drop out, and preferential amplification, may lead to misdiagnosis in preimplantation genetic diagnosis of single-gene disorders.
Methods:
Preimplantation genetic diagnosis was performed by sequential first and second polar body analysis of oocytes in 26 couples at risk for having children with various single-gene disorders. Mutant genes were amplified simultaneously with linked polymorphic markers, and only embryos resulting from the mutation-free oocytes predicted by polar body analysis with confirmation by polymorphic marker testing were transferred back to patients.
Results:
Overall 529 oocytes from 48 clinical cycles (26 patients) were tested, resulting in the transfer of 106 embryos in 44 clinical cycles. As many as 46 (9.6%) instances of allele dropout were observed, the majority (96%) of which were detected. Seventeen unaffected pregnancies were established, of which nine resulted in the birth of an unaffected child, and the rest are ongoing.
Conclusions:
A high accuracy of preimplantation genetic diagnosis of single-gene disorders is achieved by application of sequential analysis of the first and second polar body and multiplex polymerase chain reaction. 相似文献
152.
This survey of the atom packing in the high resolution X-ray crystal structures of 21 proteins indicates that the atom density around a given central atom is determined primarily by its covalently bonded neighbors and proximity to the surface of the protein. Long-range hydrophobic, hydrogen bonding, and electrostatic interactions are strictly of secondary importance. Both radial and angular atom densities were calculated about various central atoms of several residue types, averaged over all occurrences of the chosen residue type in all 21 proteins. Polar interactions, such as hydrogen bonding, involve on the average shorter distances than hydrophobic interactions do, and are more directional. Spatial segregation of polar and non-polar atoms is never complete in long-range interactions since the types of atoms are linked together covalently. 相似文献
153.
Gutiérrez-Mateo C Benet J Starke H Oliver-Bonet M Munné S Liehr T Navarro J 《Human reproduction (Oxford, England)》2005,20(12):3395-3401
BACKGROUND: Metaphase II (MII) chromosome complements are difficult to karyotype. The objective of this study was to investigate the efficiency and limitations of centromere-specific multiplex fluorescence in situ hybridization (cenM-FISH), a new 24 colour FISH technique using centromere-specific probes, to analyse the whole chromosome complement within human oocytes. METHODS: Oocytes were donated by 34 patients undergoing ovarian stimulation and IVF. The MII oocytes were analysed by means of cenM-FISH, while the confirmation of results was performed by FISH and/or by analysing the corresponding first polar bodies using comparative genomic hybridization (CGH). RESULTS: A total of 30 cells, corresponding to 16 oocytes and 14 first polar bodies, were successfully karyotyped by either cenM-FISH or CGH. The incidence of aneuploidy was 25%, and eight out of nine aneuploidy events were confirmed by CGH and FISH. CONCLUSIONS: We demonstrate here for the first time that the identification of any numerical abnormality in oocytes is feasible using cenM-FISH. Despite the fact that the fixation efficiency remains low, the present results confirm the advantage of analysing the whole set of chromosomes to make an accurate estimation of the aneuploidy rate in human oocytes. 相似文献
154.
目的应用全染色体涂抹探针(whole chromosome painting probe,WCP)对女性罗伯逊易位携带者进行卵母细胞第一极体的植入前遗传学诊断(preimplantation genetic diagnosis,PGD)。方法应用全染色体涂抹探针进行第一极体荧光原位杂交,对4例女方罗伯逊易位携带者进行了4个周期的PGD。患者染色体核型均为45,XX,der(13;14),(q10;q10)。所有周期取卵后6h内通过活检取出第一极体,采用WCP探针进行荧光原位杂交,受精后第3天选择染色体组成正常或平衡的胚胎进行宫腔内移植。结果4个周期共获卵61个,其中54个成熟可进行活检,活检成功率92.6%(50/54),固定成功率90.O%(45/50)。40个获得明确诊断,总体诊断率为74.1%(40/54)。卵胞浆内单精子注射后受精率64.8%(35/54),优质胚胎率为65.7%(23/35)。获得2例临床妊娠。其中1例于孕9周胚胎停止发育,绒毛染色体分析核型为45,X;另1例产前诊断证实核型为46,XX。2006年6月足月分娩一正常活女婴。结论全染色体涂抹探针可准确区分正常、平衡以及异常卵子,从而可有效应用于女性染色体易位携带者的PGD。 相似文献
155.
Gutiérrez-Mateo C Benet J Wells D Colls P Bermúdez MG Sánchez-García JF Egozcue J Navarro J Munné S 《Human reproduction (Oxford, England)》2004,19(12):2859-2868
BACKGROUND: The object of this study was to determine the mechanisms that produce aneuploidy in oocytes and establish which chromosomes are more prone to aneuploidy. METHODS: A total of 54 oocytes from 36 women were analysed. The whole chromosome complement of the first polar body (1PB) was analysed by comparative genomic hybridization (CGH), while the corresponding metaphase II (MII) oocyte was analysed by fluorescence in situ hybridization (FISH) to confirm the results. RESULTS: Matched CGH-FISH results were obtained in 42 1PB-MII doublets, of which 37 (88.1%) showed reciprocal results. The aneuploidy rate was 57.1%. Two-thirds of the aneuploidy events were chromatid abnormalities. Interestingly, the chromosomes more frequently involved in aneuploidy were chromosomes 1, 4 and 22 followed by chromosome 16. In general, small chromosomes (those equal to or smaller in size than chromosome 13) were more prone to aneuploidy (chi2-test, P=0.07); 25% of the aneuploid doublets would have been misdiagnosed as normal using FISH with probes for nine-chromosomes. CONCLUSIONS: The combination of two different techniques, CGH and FISH, for the study of 1PB and MII allowed the identification and confirmation of any numerical chromosome abnormality, as well as helping to determine the mechanisms involved in the genesis of maternal aneuploidy. 相似文献
156.
Gutiérrez-Mateo C Gadea L Benet J Wells D Munné S Navarro J 《Human reproduction (Oxford, England)》2005,20(5):1256-1260
In translocation carriers, the presence of aneuploidy for the chromosomes unrelated to the rearrangement may lead to an additional risk of abnormal pregnancy or implantation failure. Consequently, it may be important to analyse not only the chromosomes involved in the rearrangement but also the rest of chromosomes. We combined spectral karyotyping (SKY) and comparative genomic hybridization (CGH) to karyotype one unfertilized oocyte and its first polar body (1PB) from a Robertsonian translocation carrier t(13;14) aged 29 years who was undergoing IVF and preimplantation genetic diagnosis (PGD) for translocations and aneuploidy screening. Two out of four embryos were aneuploid, as a result of an adjacent segregation. The unfertilized oocyte had a normal/ balanced constitution of the chromosomes involved in the reorganization. However, this 1PB-metaphase II doublet was aneuploid for chromosome 12, the oocyte being hyperhaploid (24, X, +12) and its 1PB hypohaploid (22, X, -12). The application of CGH for the study of Robertsonian translocations of maternal origin will be useful to study imbalances of the chromosomes involved in the rearrangement, as well as alterations in the copy number of any other chromosome. The combination of PGD for translocations with aneuploidy screening could help to reduce the replacement of chromosomally abnormal embryos. 相似文献
157.
Magli MC Gianaroli L Ferraretti AP Toschi M Esposito F Fasolino MC 《Human reproduction (Oxford, England)》2004,19(5):1163-1169
BACKGROUND: The biopsy of both polar bodies and a blastomere from the same embryo was investigated as an approach aimed at increasing the quantity of DNA available for genetic analysis in preimplantation embryos. METHODS: In 113 cycles, preimplantation genetic diagnosis (PGD) was performed for aneuploidy: 19 cycles underwent polar body biopsy, 32 cycles had both polar body and blastomere biopsy done, and the remaining 62 cycles underwent blastomere biopsy. The chromosomal analysis was performed in a two-round fluorescence in situ hybridization (FISH) protocol with probes specific for the chromosomes X, Y, 13, 15, 16, 18, 21 and 22. RESULTS: The morphological evaluation of the analysed embryos demonstrated similar rates of development irrespective of the biopsy procedure. Accordingly, the implantation rate did not differ significantly in the three biopsy groups and was 15% after polar body biopsy, 26% after the combined biopsy procedures of polar bodies and blastomeres, and 25% after blastomere biopsy. CONCLUSIONS: The removal of a blastomere subsequent to polar body biopsy does not seem to have negative effects on embryo viability. This approach could be especially valuable for a combined diagnosis of aneuploidy and single-gene disorders in preimplantation embryos generated by couples at high reproductive risk. 相似文献
158.
《Journal of vascular and interventional radiology : JVIR》2022,33(6):695-701
PurposeTo assess the utility of the radius, exophytic/endophytic, nearness to collecting system or sinus, anterior/posterior, and location relative to polar lines (RENAL) nephrometry scoring system at predicting adverse events and outcomes in percutaneous microwave ablation (MWA) of renal tumors.Materials and MethodsA retrospective review of 116 patients who underwent MWA from 2004 to 2018 at 2 large university hospitals was conducted. Patient demographics and tumor characteristics were collected. The RENAL nephrometry scores were calculated, and procedure-related adverse events were stratified into minor and major (the Society of Interventional Radiology classification of class C or higher). Technical and oncologic outcomes were based on follow-up magnetic resonance imaging and computed tomography scans after ablation.ResultsThe mean RENAL score was 6.6 (range, 4–11), and the mean tumor size was 24 mm. Follow-up ranged between 16 and 161 weeks (median, 50 weeks; mean, 65 weeks). Oncologic control was achieved in 96% (n = 111) of patients. The major and minor adverse event rates were 8.6% (n = 10) and 17% (n = 19), respectively. The mean RENAL score for patients with recurrent and/or residual tumor (8.2 ± 2.7) was higher than that for patients without disease recurrence (6.5 ± 3.5, P = .05). However, in a multivariate analysis, the RENAL score was not found to be an independent predictor of oncologic outcomes (odds ratio, 1.548; P = .092).ConclusionsThe RENAL nephrometry score has minimal utility for predicting outcomes and adverse events in MWA of renal tumors. The inconsistent nature of RENAL nephrometry scoring in percutaneous ablation procedures underscores the need for an ablation-specific risk stratification system. 相似文献
159.
不同成熟时间对牛卵母细胞发育及去核效率的影响 总被引:6,自引:0,他引:6
本文研究了不同成熟时间对牛卵母细胞的体外成熟、体外受精及后期的体外培养的影响和不同成熟时间的极体出现率。结果表明,牛卵母细胞在成熟16h时极体出现率达69.3%、分裂率为54.7%、囊胚率仅为6.25%;而成熟24h时极体出现率达76.9%、分裂率为70.0%、囊胚率为27.7%,差异均显著(P<0.05)。成熟16h的卵母细胞刚刚排出第一极体,细胞核与极体非常近,此时去核率较高;而成熟24h的卵母细胞核已远离第一极体。 相似文献
160.