妊高征母胎界面血管紧张素Ⅱ水平测定及意义

目的 检测血管紧张素(ANG)Ⅱ在妊高征(PIH)及正常孕妇母血、脐带血以及胎盘母面和子面的水平,探讨PIH发病因素及胎儿宫内生长迟缓(IUGR)的发生机制.方法 选取PIH和正常孕妇各30例,以ELISA法检测母血、脐带血、胎盘母面及子面组织中ANGⅡ的含量,对两样本进行t检验和Pearson相关分析.结果 妊高征和正常孕母外周血ANGⅡ含量分别为(46.44±8.48)pg/ml、(32.43±5.87)pg/ml,两者差异显著(P＜0.001);妊高征和正常孕母脐带血ANGⅡ含量分别为(68.83±8.68)pg/ml、(72.47±8.51)pg/ml,两者无统计学差异(P＞0.05);妊高征和正常孕母胎盘母面ANGⅡ含量分别为(8.51±4.01)pg/ml、(7.76±3.47)pg/ml,两者无统计学差异(P＞0.05);妊高征和正常胎盘子面ANGⅡ含量分别为(11.82±3.92)pg/ml、(9.64±2.63)pg/ml,两者差别显著(P＜0.05);妊高征脐带血ANGⅡ水平与母亲外周血ANGⅡ水平相关系数为0.7379,P＜0.05.结论 妊高征患者外周血及胎盘子面ANGⅡ水平升高,其脐带血中的ANGⅡ水平与母血的ANGⅡ水平正相关.     

Stillbirth after COVID-19 in Unvaccinated Mothers Can Result from SARS-CoV-2 Placentitis,Placental Insufficiency,and Hypoxic Ischemic Fetal Demise,Not Direct Fetal Infection: Potential Role of Maternal Vaccination in Pregnancy

Stillbirth is a recently recognized complication of COVID-19 in pregnant women. Other congenitally transmitted infections from viruses, bacteria and parasites can cause stillbirth by infecting fetal organs following transplacental transmission of the agent from the maternal bloodstream. However, recent research on pregnant women with COVID-19 having stillbirths indicates that there is another mechanism of stillbirth that can occur in placentas infected with SARS-CoV-2. In these cases, viral infection of the placenta results in SARS-CoV-2 placentitis, a combination of concurrent destructive findings that include increased fibrin deposition which typically reaches the level of massive perivillous fibrin deposition, chronic histiocytic intervillositis and trophoblast necrosis. These three pathological lesions, in some cases together with placental hemorrhage, thrombohematomas and villitis, result in severe and diffuse placental parenchymal destruction. This pathology can involve greater than one-half of the placental volume, averaging 77% in the largest study of 68 cases, effectively rendering the placenta incapable of performing its function of oxygenating the fetus. This destructive placental process can lead to stillbirth and neonatal death via malperfusion and placental insufficiency which is independent of fetal infection. Fetal autopsies show no evidence that direct infection of fetal organs is contributory. Because all mothers examined have been unvaccinated, maternal vaccination may prevent viremia and consequent placental infection.     

Twin anemia‐polycythemia sequence with suspected cardiac dysfunction

We present a case of twin anemia‐polycythemia sequence diagnosed postnatally with suspected chronic stress on the heart in the donor twin.     

Dendritic polyglycerol nanoparticles show charge dependent bio-distribution in early human placental explants and reduce hCG secretion

A thorough understanding of nanoparticle bio-distribution at the feto-maternal interface will be a prerequisite for their diagnostic or therapeutic application in women of childbearing age and for teratologic risk assessment. Therefore, the tissue interaction of biocompatible dendritic polyglycerol nanoparticles (dPG-NPs) with first- trimester human placental explants were analyzed and compared to less sophisticated trophoblast-cell based models. First-trimester human placental explants, BeWo cells and primary trophoblast cells from human term placenta were exposed to fluorescence labeled, ～5?nm dPG-NPs, with differently charged surfaces, at concentrations of 1 µM and 10?nM, for 6 and 24?h. Accumulation of dPGs was visualized by fluorescence microscopy. To assess the impact of dPG-NP on trophoblast integrity and endocrine function, LDH, and hCG releases were measured. A dose- and charge-dependent accumulation of dPG-NPs was observed at the early placental barrier and in cell lines, with positive dPG-NP-surface causing deposits even in the mesenchymal core of the placental villi. No signs of plasma membrane damage could be detected. After 24?h we observed a significant reduction of hCG secretion in placental explants, without significant changes in trophoblast apoptosis, at low concentrations of charged dPG-NPs. In conclusion, dPG-NP’s surface charge substantially influences their bio-distribution at the feto-maternal interface, with positive charge facilitating trans-trophoblast passage, and in contrast to more artificial models, the first-trimester placental explant culture model reveals potentially hazardous influences of charged dPG-NPs on early placental physiology.     

前置胎盘剖宫产产后出血行欣母沛与宫腔填纱联合治疗的效果分析

目的探讨前置胎盘剖宫产产后出血行欣母沛与宫腔填纱联合治疗的效果,以便为前置胎盘剖宫产产后出血的临床治疗提供理论与实践依据。方法选取2016年1月～2018年1月本院妇产科收治的前置胎盘剖宫产产后出血患者合计93例进行回顾性分析,在经本院医学伦理委员会批准的基础上按随机数字表法(RNTM)将其分成三组,联合组采用欣母沛与宫腔填纱联合治疗,对照组单纯采用欣母沛治疗,实验组单纯采用宫腔填纱治疗,比较三组患者术中、术后出血量,血压、体温等指标的变化情况。结果联合组术中出血量与对照组和实验组相比均有统计学差异(P0.05),对照组与实验组相比无明显差异(P0.05);联合组术后2 h、24 h出血量与实验组、对照组相比有统计学差异(P0.05),实验组与对照组相比有差异(P0.05)。三组患者治疗后血压、体温比较,联合组优于实验组及对照组,有统计学差异(P0.05),三组治疗前无明显差异(P0.05)。对照组与实验组并发症发生率比较无显著差异(P0.05),联合组的并发症发生率均低于实验组、对照组,差异显著(P0.05)。结论在前置胎盘剖宫产产后出血患者的临床治疗中,欣母沛与宫腔填纱联合与单纯的予以欣母沛或宫腔填纱相比临床疗效更佳,有良好的临床应用价值。