基于网络药理学研究左金丸治疗胃食管反流病分子机制

目的:基于网络药理学分析左金丸治疗胃食管反流病的作用机制。方法:通过TCMSP检索左金丸的有效成分及蛋白靶点,在Uniprot数据库中将蛋白靶点名称转化为基因名,运用Cytoscape 3.7.1绘制左金丸有效成分-靶点-药物网络并进行拓扑参数分析,通过在线人类孟德尔遗传数据库（OMIM）、GeneCards、DRUG...     

核桃楸皮的化学成分

目的对中药核桃楸(Juglans MandshuricaMaxim.)皮的化学成分进行了分离、鉴定。方法核桃楸皮的水提取物用硅胶色谱法、聚酰胺柱色谱、凝胶柱色谱、反相ODS柱色谱和制备HPLC方法进行分离纯化;根据波谱数据解析、理化常数分析及文献对照进行结构鉴定。结果从核桃楸的水提取物中分离鉴定了6个化合物,分别是8羟基蒽醌1羧酸(1)、山柰酚(2)、杨梅苷(3)、槲皮苷(4)、4,5,8三羟基α萘酮5OβD吡喃葡萄糖苷(5)、4,5,8三羟基α萘酮5OβD(6′O没食子酰基)葡萄糖苷(6)。结论化合物1为新天然产物,化合物1~4为首次从核桃楸中分离得到。     

Analgesic properties of a hydroalcoholic extract obtained from Alternanthera brasiliana

The present study describes the analgesic effects of the hydroalcoholic extract (HE) obtained from the aerial parts of Alternanthera brasiliana in two models of pain in mice. Such an extract, given intraperitoneally or orally produced significant and long-lasting (0.5–4 h) antinociception when evaluated against acetic acid-induced abdominal constrictions. In the formalin test, the HE inhibited both the first and second phases of formalin-induced pain. Furthermore, the HE was more potent than some standard drugs, such as aspirin, indomethacin and dipyrone, when evaluated against acetic acid-induced abdominal constrictions. These results suggest a strong analgesic effect for HE, possibly related to the presence of sterols, terpenes and phenolic compounds, confirming the popular use of A. brasiliana against dolorous processes. © 1998 John Wiley & Sons, Ltd.     

A review of the plants of the genus Phyllanthus: Their chemistry,pharmacology, and therapeutic potential

The plants of the genus Phyllanthus (Euphorbiaceae) are widely distributed in most tropical and subtropical countries, and have long been used in folk medicine to treat kidney and urinary bladder disturbances, intestinal infections, diabetes, and hepatitis B. In recent years, the interest in the plants has increased considerably. Substantial progress on their chemistal and pharmacological properties, as well as a few clinical studies of some Phyllanthus species have been made. This review discusses the current knowledge of their chemistry, the in vitro and in vivo pharmacological, biochemical, and clinical studies carried out on the extracts, and the main active constituents isolated from different species of plants of the genus Phyllanthus. These studies carried out with the extracts and purified compounds from these plants support most of their reported uses in folk medicine as an antiviral, in the treatment of genitourinary disorders, and as antinociceptive agents. However, well-controlled, double-binding clinical trials are lacking. Several compounds including alkaloids, flavonoids, lignans, phenols, and terpenes were isolated from these plants and some of them interact with most key enzymes. Together this data strongly supports the view that the plants belonging to the genus Phyllanthus have potential beneficial therapeutic actions in the management of hepatitis B, nefrolitiase, and in painful disorders. © 1998 John Wiley & Sons, Inc., Med Res Rev, 18, No. 4, 225–258, 1998.     

石榴的药理作用与安全性研究进展

石榴为石榴科石榴属植物,原产于伊朗和印度北部间的区域,栽培历史悠久。因其花果美丽,果实鲜美可口,深受人们喜爱。石榴营养丰富,且具有广泛的药用价值。本文介绍了石榴各部位所含的主要活性成分及其抗氧化、抗微生物、抗癌、抗紫外辐射以及改善代谢综合症等药理活性的研究进展,概述了石榴活性成分间的协同作用以及石榴提取物的安全性,对加强石榴相关药理活性和质量标准的研究,及开发石榴相关产品方面具有重要意义,对石榴资源的综合开发利用也具有重要价值。     

藿香正气水气相色谱指纹图谱特性研究

目的:建立藿香正气水的GC指纹图谱。方法:采用水蒸气蒸馏和正己烷萃取藿香正气水,以正十七烷为参照物经毛细管气相色谱柱(含FID检测器)进行了分析。结果:对10批次藿香正气水供试品进行检测,建立了该药品的GC指纹图谱并标示了16个共有指纹峰。各共有峰相对保留时间变化的RSD均在0.5%以内。结论:该方法准确、重现性好,为进一步质量标准化研究和控制提供依据。     

大叶冬青的三萜皂苷类成分研究

 目的 对中药苦丁茶主要品种大叶冬青叶的三萜皂苷类成分进行研究。方法 采用硅胶、葡聚糖凝胶LH-20和反相ODS柱色谱分离大叶冬青叶的水提物,通过光谱分析结合理化性质鉴定化合物的结构。结果 从大叶冬青的水提液中分离得到11个化合物,分别鉴定为α-苦丁内酯3-O-β-D-吡喃葡萄糖(1→3)[α-L-吡喃鼠李糖(1→2)]-α-L-吡喃阿拉伯糖苷(Ⅰ)、α-苦丁内酯3-O-β-D吡喃葡萄糖 (1→2)-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖苷 (Ⅱ)、3-O-β-D-吡喃葡萄糖 (1→2)-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖斯阿树脂酸28-α-L-吡喃鼠李糖 (1→2)-β-D-吡喃葡萄糖酯苷(Ⅲ)、3-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖坡模醇酸28-α-L-吡喃鼠李糖 (1→2)-β-D-吡喃葡萄糖酯苷 (Ⅳ)、β-苦丁内酯3-O-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖苷(Ⅴ)、β-苦丁内酯3-O-β-D-吡喃葡萄糖 (1→2)-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖苷(Ⅵ)、γ-苦丁内酯3-O-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖苷(Ⅶ)、3-O-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖齐墩果酸28-α-L-吡喃鼠李糖 (1→2)-β-D-吡喃葡萄糖酯苷(Ⅷ)、3-O-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖斯阿树脂酸28-α-L-吡喃鼠李糖 (1→2)-β-D-吡喃葡萄糖酯苷(Ⅸ)、3-O-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖-(1→2)]-α-L-吡喃阿拉伯糖坡模醇酸28-α-L-吡喃鼠李糖 (1→2)- β-D-吡喃葡萄糖酯苷(Ⅹ)及δ-苦丁内酯3-O-β-D-吡喃葡萄糖 (1→2)-β-D-吡喃葡萄糖 (1→3)-[α-L-吡喃鼠李糖 (1→2)]-α-L-吡喃阿拉伯糖苷(Ⅺ)。结论 化合物Ⅰ～Ⅳ、Ⅵ、Ⅶ及Ⅺ为首次从该植物中分离得到。     

阿魏的化学成分研究

目的:研究准格尔阿魏Ferula songorica Pall.ex Spren in Roem.et Schult的化学成分.方法:利用各种色谱方法进行分离纯化,根据波谱解析、理化常数分析及文献对照进行结构鉴定.结果:从阿魏根茎95%乙醇提取物中分离得到3个化合物,分别为2,4-二羟基-α-氧代-苯乙酸(1),3,3',4,4'-联苯四甲酸(2),2,4-二羟基苯甲酮(3).结论:化合物2是新天然产物,化合物1为首次从该植物中分离得到.     

Long term exposure effect of a unique metabolic nutrition system containing a diverse group of phytochemicals on serum chemistry and genomic and non-genomic changes in the liver of female B6C3F1 mice

The nutritional value and therapeutic benefits coupled with presumed safety have heightened interest in the use of custom designed dietary supplements. Their use has increased substantially since the passage of the 1994 Dietary Supplement Health Education Act. However, few well-controlled studies have been conducted to assess the safety and potential adverse effects of dietary supplements. MNSO (MNS Orange-AdvoCare) is a unique combination of vitamins, minerals, omega-3 fatty acids and herbal extracts designed to provide a strong foundation of nutritional support, enhance thermogenesis, heighten energy levels and improve immune status. This investigation was designed to explore the safety and toxic effects, if any, of 12 months of continuous exposure to the ephedra and caffeine containing MNSO on serum chemistry and histopathology of seven vital target organs in female B6C3F1 mice. MNSO is enriched with extracts of citrus, Ephedra, Ginkgo, green tea and Ocimum. In this study, mice were fed control (-MNSO) or MNSO (1x-10x, 1x = daily human dose) diets. Blood was collected from all groups including the control every 4 months for serum chemistry analysis (enzyme, lipid, carbohydrate, electrolyte profiles), and liver tissue was collected for tissue biochemistry and histopathology. Multiple biochemical parameters included: (i) determination of oxidative stress via lipid peroxidation and (ii) assessment of genomic integrity via DNA fragmentation. In addition, food consumption and body weight changes were also monitored biweekly. The data showed that 12 months ingestion of 10x-MNSO did not significantly influence organ histopathology, alter the normal serum chemistry profile or any of the crucial tissue biochemistry. MNSO-exposed animals were more active, consumed more food and were relatively leaner compared with the controls. This study indicates that a caffeine and ephedra containing metabolic nutrition system is non-toxic and non-hepatotoxic in mice at up to 10x the human consumption dose of ephedra.     

掌叶苹婆化学成分的研究

目的:研究掌叶苹婆Sterculia foetida的化学成分.方法:通过各种柱色谱进行分离纯化,根据理化性质和波谱数据进行结构鉴定.结果:分离并鉴定了8个化合物:5,7,8-三羟基-4'-甲氧基黄酮-8-O-β-D-葡萄糖苷(1),5,7,8-三羟基-4'-甲氧基黄酮-7-O-β-D-葡萄糖苷(2),槲皮素-3-O-β-D-葡萄糖苷(3),芹菜素-6,8-二葡萄糖碳苷(4),葛根素(5),5,7,8,3'-四羟基-4'-甲氧基黄酮(6),5,7,8.三羟基-3',4'-二甲氧基黄酮(7),5,7,8-三羟基4'-甲氧基黄酮(8).结论:化合物1,2,4-8为首次从掌叶苹婆中分离得到.