多囊卵巢综合征胰岛素抵抗与痰湿的关系

通过广泛查阅相关文献,并结合中医经典理论进行探讨,认为痰湿内盛是多囊卵巢综合征(PCOS)的主要病机,痰湿体质是本病胰岛素抵抗(IR)和代谢紊乱发生的重要病理基础,本病痰湿证患者胰岛素抵抗更加明显,中医药从痰论治多囊卵巢综合征所取得的疗效,也印证了本病"痰壅胞宫"之病机。     

由"病痰饮者,当以温药和之"论仲景治痰饮

"病痰饮者,当以温药和之",是张仲景提出的治疗痰饮病的大法.脾失健运,水精不能四布,是引起痰饮病的主要原因,总属阳虚阴盛,本虚标实之候.温通阳气为治本之法,另外,根据痰饮之部位不同又有发汗、利小便、攻逐水饮、扶正祛饮之法.     

基于数据挖掘探讨吴寿善治疗慢性乙型病毒性肝炎用药规律

目的：基于中医医疗与临床科研信息共享系统,运用数据挖掘技术探讨湖北中医大师吴寿善教授 中医药治疗慢性乙型病毒性肝炎的用药规律。方法：应用结构化电子病历采集767例吴寿善接诊慢性乙型病 毒性肝炎门诊患者的临床资料,通过数据集成、汇交、整合后,利用数据分类、关联、聚类规则方法对临床资料进 行分析。结果：①确定259个处方中药物的使用频次前五位药物分别是茯苓、茵陈、陈皮、丹参、连翘;②药物配 伍方面,关联度最高的配对分别为茯苓-甘草-白术、茯苓-丹参-白术、茵陈-茯苓-甘草等;③挖掘核心处方时, 发现蒿芩清胆汤、大安丸、半夏白术天麻汤等经典方加减使用频次最高。结论：通过数据挖掘方法,我们发现吴 寿善教授以中医药治疗慢性乙型病毒性肝炎在治则治法上,主要从毒痰瘀虚进行辨证施治。     

The atomic force microscope: a new tool for artificial organ research

The atomic force microscope (AFM) was developed by modifying the scanning tunneling microscope (STM). It has high resolution on the subnanometer scale (10⁻¹⁰ m), does not require troublesome preprocessing of the sample, and permits observation of living samples. With these attractive features, the AFM is expected to be a new research tool in the field of artificial organs in the near future. This review describes the history and mechanism of the AFM and some of our observations of biological samples.     

Two‐ and three‐dimensional multinuclear stray‐field imaging of rotating samples with magic‐angle spinning (STRAFI‐MAS): From bio to inorganic materials

Purpose:

To revisit and illustrate the potential of a simple and effective multidimensional stray‐field imaging technique with magic‐angle spinning, known as STRAFI‐MAS.

Materials and Methods:

STRAFI‐MAS images are acquired with a standard NMR magnet and a traditional magic‐angle sample spinning (MAS) probe. The stray‐field gradients are achieved by placing the MAS probe, along the z‐direction, at a distance from the center of the magnet. No pulsed‐field gradients are applied. The multidimensional spatial encoding is carried out by synchronizing the radiofrequency pulses with the sample MAS rotation.

Results:

Two‐dimensional (2D) and 3D multinuclear images of various phantoms, including a tibia bone and silicon carbide, are recorded. Images of inorganic solids containing quadrupolar nuclei, ²³Na and ²⁷Al, are also explored for the first time by STRAFI‐MAS.

Conclusion:

We have demonstrated that STRAFI‐MAS is a simple and user‐friendly technique for multidimensional imaging without the need of imaging equipment. With the current advancements in NMR and MRI methodologies, STRAFI‐MAS is expected to be further developed and improved. We anticipate that STRAFI‐MAS can spark a wide spectrum of interest, from material to bio science, where can benefit from high‐resolution images. J. Magn. Reson. Imaging 2010;32:418–423. © 2010 Wiley‐Liss, Inc.     

丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制研究

目的初步探析丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制。方法选取健康雌性豚鼠24只,按体质量分层随机分为4组：空白对照组、模型对照组、地塞米松组、丹龙定喘汤组,每组6只。采用卵蛋白致敏法建立豚鼠哮喘气道重塑模型,以丹龙定喘汤干预,并以地塞米松做对照药物,治疗1个月后分别检测气道重塑的程度及豚鼠肺组织中转化生长因子β1（TGF-β1）含量。结果 （1）模型对照组豚鼠肺支气管管壁和平滑肌层明显增厚,与空白对照组相比差异有统计学意义（P〈0.05）;丹龙定喘汤组气道壁厚度减轻,与模型对照组相比差异有统计学意义（P〈0.05）,与地塞米松组相比,差异无统计学意义（P〉0.05）。（2）模型对照组豚鼠肺组织中的TGF-β1含量明显增加,与空白对照组相比,差异有统计学意义（P〈0.05）;丹龙定喘汤组TGF-β1含量减少,与模型对照组相比差异有统计学意义（P〈0.05）,与地塞米松组相比差异无统计学意义（P〉0.05）。结论丹龙定喘汤对哮喘豚鼠气道重塑有一定抑制作用,其机制可能是通过下调哮喘豚鼠肺组织中的TGF-β1水平,抑制气道平滑肌细胞及细胞外基质的增殖而阻断气道重塑的进程。     

老年慢性阻塞性肺疾病急性期合并肺结核感染的治疗方法及其疗效探讨

目的：探讨老年慢性阻塞性肺疾病(chronic obstructive lung disease,COPD)急性期合并肺结核感染的治疗方法及其疗效。方法：选取2014年2月至2016年2月我院收治的老年慢性阻塞性肺疾病急性期合并肺结核感染的患者92例,根据治疗方式随机分为治疗组和对照组,对照组给予COPD对症支持治疗和抗肺结核治疗,治疗组在对照组基础上加用痰热清,比较两组患者治疗有效率、痰培养阴转率以及肝肾损害情况的差异。结果：治疗组患者治疗后痰培养阴转率(36例,85.71%)与对照组(41例,82.0%)的差异无统计学意义(P>0.05)。两组患者治疗后的症状积分均低于治疗前,且差异具有统计学差异(P<0.05),治疗组治疗后的症状积分(85.62±11.37)低于对照组(91.25±12.38),且差异具有统计学差异(P<0.05)。治疗组治疗后痊愈2例(4.76%),显效26例(61.90%),有效10例(23.81%),无效4例(9.52%);对照组治疗后痊愈1例(2.0%),显效17例(34.0%),有效22例(44.0%),无效10例(20.0%);治疗组治疗疗效明显优于对照组,且差异具有统计学意义(P<0.05)。治疗组治疗后发生肝肾损害14例,对照组11例,两组患者治疗后肝肾损害发生率的差异无统计学意义(P>0.05)。结论：在不影响痰培养阴转率和不加重肝肾负担的情况下,老年COPD急性期合并肺结核感染患者接受一般性治疗的同时进行中医治疗,其疗效更为显著。     

Determination of metolcarb residues by a biotin–streptavidin-amplified enzyme-linked immunosorbent assay in vegetables and edible fungus

An effective biotin–streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) to rapidly detect metolcarb (MTMC) residues is reported. Nonspecific adsorption was minimized by using 0.5% skimmed milk powder as a blocking buffer and 0.5% bovine serum albumin/phosphate-buffered saline (PBS) as a buffer for streptavidin–horse radish peroxidase conjugates. The established method is four times sensitive than direct competitive enzyme-linked immunosorbent assay, with the IC₅₀ of 10.0?ng?mL^?1. The samples were prepared from mustard, cucumber and mushroom with simple extraction and dilution methods, including use of PBS without concentration or cleanup steps. The samples prepared from spinach and shiitake were quantified after 2-fold methanol extraction and 20-fold dilution with 2.0% fish glutin/PBS. Good accuracy and precision were obtained with mean recoveries between 80.0% and 95.6%, and mean coefficients of variation below 12.1%. A good correlation (R^2?=?0.9931) between the BA-ELISA and high-performance liquid chromatography was observed for MTMC analysis in different samples. This method could be potentially useful for high-throughput food inspection.     

四种中药注射液对常用生化检验项目的分析干扰筛选

目的 探讨丹红注射液、丹参酮Ⅱa磺酸钠注射液、灯盏花素注射液、喜炎平注射液对常用生化检验项目是否存在体外分析干扰.方法 收集当日我院体检中心3口内未服用任何药物的健康体检者混合血清做基础样本,取基础样本20倍稀释四种药物原液作为实验样本.取基础样本20倍稀释注射用水做对照样本,分别测定丙氨酸氨基转移酶(AST)、天门冬氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、尿素(UREA)、肌酐(CREA)、尿酸(UA)、总胆固醇(TCHO)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)a-羟丁酸脱氢酶(a-HBDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB),通过计算得出4种药物对各检验项目是否存在干扰可能.结果 四种药物中丹红注射液对生化项目UREA、CREA、UA、TG、TCHO、HDL-C、a-HBDH、LDL-C、CK、CK-MB存在分析干扰可能,丹参酮Ⅱa磺酸钠注射液对实验中的生化项目不存在分析干扰可能,喜炎平注射液对生化项目LDH存在分析干扰可能,灯盏花素注射液对生化检验项目CREA、UA、TG、TCHO、HDL-C、LDL-C、LDH存在分析干扰可能.结论 四种中药注射液中丹红注射液、喜炎平注射液和灯盏花素注射液对部分生化检验项目存在干扰可能.     

Movement preparation and working memory: a behavioural dissociation

We can cross temporal sensorimotor contingencies by remembering sensory events or by anticipating motor responses. Here we tested the hypothesis that sensory and motor representations can be accessed according to different temporal dynamics. We predicted that the manipulation of movement representations would lead to a performance independent from the length of a delay interposed between sensory instructions and behavioural responses. Conversely, we expected a delay-dependent performance whenever temporary storage of sensory information was necessary to solve the task. We have measured reaction times and error rate in subjects performing a delayed non-matching to sample task. Task contingencies rather than explicit instructions ensured that either sensory or motor representations were used to cross the delay period on each trial. We tested our hypothesis by manipulating the length of the delays between stimulus presentation and behavioural response. We found that carrying sensory material over temporal gaps affects performance as a non-linear function of time, whereas movement representations remain robust over a wide range of delays. This novel behavioural paradigm might prove effective in dissociating the neural bases of preparatory and mnestic processes in normal human subjects, as well as their disorders in neurological patients.