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991.
Hitzeroth A Niehaus DJ Koen L Botes WC Deleuze JF Warnich L 《Progress in neuro-psychopharmacology & biological psychiatry》2007,31(3):664-672
Reactive oxygen species (ROS)-mediated damage has been hypothesized to play a role in the development and poor outcome of schizophrenia, as well as the development of neuroleptic-induced abnormal involuntary movements. Recently, the functional polymorphism (Ala-9Val) in the manganese superoxide dismutase (MnSOD) gene (part of the antioxidant defense mechanism) was found to be associated with schizophrenia in a Turkish population. This study was aimed at replicating this finding in a Xhosa population. In addition, the role of Ala-9Val in abnormal involuntary movement and tardive dyskinesia development in the Xhosa population was also investigated. The schizophrenic patient group (n=286) and a healthy control group (n=243) were genotyped for the Ala-9Val polymorphism using heteroduplex-single stranded conformational polymorphism (HEX-SSCP) analysis. No significant difference in genotype or allele frequency could be observed between the schizophrenia and control group (P=0.294 and P=0.528 respectively). In addition no association could be found between the polymorphism and symptom severity (SANS and SAPS). The Xhosa schizophrenia patient group with abnormal involuntary movements (n=54) and a subgroup with tardive dyskinesia (n=30) was found to significantly differ in Ala-9Val genotype frequency (P=0.008 and P=0.011 respectively) compared to the Xhosa schizophrenia patient group without abnormal involuntary movements (n=204). However, no significant difference was found for the allele frequencies (P=0.955 and P=0.161). Further, using ANCOVA no association was found between AIMS score and genotype in the group with abnormal involuntary movements (P=0.1234). However, in the patient group with tardive dyskinesia an association was observed between genotype and AIMS score (P=0.0365). These results do not support a major role of the MnSOD Ala-9Val polymorphism in the development of schizophrenia or symptom severity in the Xhosa population. Yet it seems to be involved in the development of abnormal involuntary movements and tardive dyskinesia and may even modulate the severity of tardive dyskinesia. 相似文献
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In the present study, flexible ligand docking and multiple scoring were used to study the binding of long-chain fatty acid
(LCFAs) to the peroxisome proliferator-activated receptor α (PPARα). The calculations indicated that LCFAs bind PPARα in nanomolar
affinities, which is in agreement with the intracellular concentrations of LCFAs. Calculated binding affinities were linearly
related with the chain length up to 20 carbon atoms. The best correlation between the rank order of experimentally detected
binding affinities and the predicted scores was found with the internal coordinate mechanics (ICM) binding energy method.
This study contributes molecular insight into the binding process, which is of pivotal importance for designing new ligands
interfering with lipid and glucose homeostasis. 相似文献
996.
Dong-Yan Gao Li-Mei Han Li-Hong Zhang Xiao-Ling Fang Jian-Xin Wang 《Archives of pharmacal research》2009,32(5):773-779
Salvianolic acid B (SalB) is an active component isolated from Chinese herbal medicine Salvia miltiorrhiza. The aim of this study was to investigate the extent of absolute oral bioavailability (F) of SalB in beagle dogs and the
effect on blood viscosity after intravenous and oral administration of Salvianolic acids (SAs). A gradient elution HPLC method
was developed and validated to determine the concentration of SalB and its three possible metabolites in plasma. After SAs
(180 mg/kg, p.o.; 9 mg/kg, i.v.) were given, the AUCs of SalB were 1680 ± 670 and 7840 ± 1140 ng/mL·h, respectively. The F
of SalB in dogs was calculated to be only 1.07 ± 0.43%. The blood viscosity was remarkably decreased after a single intravenous
injection of SAs (9 mg/kg). However, no significant change of blood viscosity was observed after a single oral administration
of SAs (180 mg/kg). The results suggested that the F of SalB was extremely low and single oral administrated SAs had no effect
on ameliorating blood viscosity in beagle dogs. 相似文献
997.
Konrad Kleszczyński Andrzej C. Sk?adanowski 《Toxicology and applied pharmacology》2009,234(3):300-305
Perfluorinated (aliphatic) acids (PFAs) and congeners have many applications in various industrial fields and household for decades. Years later they have been detected in wildlife and this has spurred interest in environmental occurrence as well as influencing living organisms. PFAs were established as peroxisome proliferators and hepatocarcinogens. Amphipatic structure suggests that they may alter cell membrane potential (mbΔΨ) and/or induce changes in cytosolic pH (pHi). The aim of this study was to examine the correlation between changes of above parameters and PFAs structure (CF6-CF12) in human colon carcinoma HCT116 cells.mbΔΨ and pHi were measured by flow cytometry using fluorescence polarization of the plasma membrane probe 3,3′-dipentyloxacarbocyanine (DiOC5(3)) and fluorescein diacetate (FDA), respectively. Dose- and time-dependent manner analysis revealed relatively fast depolarization of plasma membrane and acidification of cytosol both positively correlated with fluorocarbon chain length. mbΔΨ depletion after 4 h of incubation reached 8.01% and 30.08% for 50 μM PFOA and 50 μM PFDoDA, respectively. Prolonged treatment (72 h) led to dramatic dissipation of membrane potential up to 21.65% and 51.29% and strong acidification to pHi level at 6.92 and 6.03 at the presence of above compounds, respectively.The data demonstrate that PFAs can alter plasma membrane protonotrophy with the mode dependent on the compound hydrophobicity. 相似文献
998.
Dr Vicki Strugala Jeanine Avis Ian G. Jolliffe Lesley M. Johnstone Peter W. Dettmar 《The Journal of pharmacy and pharmacology》2009,61(8):1021-1028
Objectives During a reflux event the oesophagus is exposed to a heterogeneous mixture of gastric juice components. The role of non‐acid components of the refluxate in causing damage to the oesophagus is now well established but no therapeutic option exists to address this. Methods The role of Gaviscon Advance (GA), a raft‐forming alginate suspension, in protecting the oesophagus from damage by pepsin and bile acids (aggressors) was investigated using a series of in‐vitro models. Key findings GA was able to dose‐dependently inhibit pepsin activity over and above the neutralisation effect of the formulation. This was evident against both protein and collagen substrates using two distinct colorimetric assays. GA was able to retard the diffusion of pepsin and multiple bile acids using a Franz cell model. Using the raft‐forming mode of action GA was able to remove both pepsin and multiple bile acids from a simulated reflux event. There was capacity in the GA raft to accommodate aggressors from multiple reflux events. Conclusions GA can specifically remove both pepsin and bile acids from the refluxate, limit their diffusion and affect enzymatic activity of pepsin. There is a role for GA to reduce the damaging potential of the refluxate and thus protect the oesophagus. 相似文献
999.
The periodical occurrence of harmful algal blooms (HABs) in freshwater lakes requires the determination of potential cyanobacterial toxins, especially microcystins (MCs). On demand of an adequate risk assessment, the high diversity of these hepatotoxic cyclic heptapeptides implicates the need of an unambiguous detection of their specific structural variants. Therefore, LC-MS and LC-MS/MS methods are the approaches of choice for determination of MCs. In contrast, even tandem mass spectromic fragmentation patterns are not even sufficient in any kind of structural determination requirements, whereas NMR methods require very high amounts of MCs. In this study, we present a novel method for chromatographic separation of desmethylated microcystins (dm-MCs). Based on the isolation of the specific structural variants using semi-preparative HPLC, a method was developed for the structure elucidation of cyclic peptides with special appliance for the determination of dm-MCs via analysis of the specific amino acid composition after peptide hydrolysis followed by stereospecific detection of the amino acids and resulting keto acids. On the basis of this method it is demonstrated that dm-MC-RR with the structure [Dha7]MC-RR represented the major compound in the microcystin pattern of Microcystis aeruginosa bloom events in 2005 and 2006 in Lake Senftenberg, Germany. 相似文献
1000.
Blanco S Sanz ME López JC Alonso JL 《Proceedings of the National Academy of Sciences of the United States of America》2007,104(51):20183-20188
We explored the conformational landscape of the proteinogenic amino acid serine [CH(2)OH CH(NH(2)) COOH] in the gas phase. Solid serine was vaporized by laser ablation, expanded in a supersonic jet, and characterized by Fourier transform microwave spectroscopy. In the isolation conditions of the jet there have been discovered up to seven different neutral (non-zwitterionic) structures of serine, which are conclusively identified by the comparison between the experimental values of the rotational and quadrupole coupling constants with those predicted by ab initio calculations. These seven forms can serve as a basis to represent the shape of serine in the gas phase. From the postexpansion abundances we derived the conformational stability trend, which is controlled by the subtle network of intramolecular hydrogen bonds formed between the polar groups in the amino acid backbone and the hydroxy side chain. It is proposed that conformational cooling perturbs the equilibrium conformational distribution; thus, some of the lower-energy forms are "missing" in the supersonic expansion. 相似文献