一株黑色素高产细菌的发酵条件研究

采用正交试验和单因素试验研究了黑色素高产细菌BFHM2002获得高产所需的发酵条件.结果表明其最适培养基为(%,w/v):蛋白胨2、甘露醇0.4、碳酸钙0.2、氯化钠0.5、L-酪氨酸0.1,pH6.0.在优化条件下,产量可达3.25mg/ml.     

参藿口服液药理作用的初步研究

目的 :利用小鼠研究参藿口服液的初步药理作用。方法 :游泳、避暗、自主活动度和睡眠实验。结果 :参藿口服浓缩液 5～10mL·kg^-1连续口服给药 7d ,能明显延长小鼠负重游泳时间 ,增加小鼠耐缺氧生存时间 ,对剥夺睡眠小鼠所引起的学习记忆障碍也有一定的改善作用 ;参藿口服浓缩液 5～2 0mL·kg^-1连续口服给药 10d ,可明显增加正常小鼠的自主活动度 ,并能对抗安定所致的镇静作用 ,缩短戊巴比妥钠的睡眠时间。结论 :参藿口服液对小鼠具良好的抗疲劳、抗应激、增强学习记忆等药理作用。     

甲基莲心碱的药理作用

甲基莲心碱是一种双苄基异喹啉类生物碱。该文就甲基莲心碱的扩血管、降压 ,抗心律失常 ,抗血小板聚集 ,抗血栓形成 ,抗氧化 ,抗有机磷农药中毒 ,抗瘢痕形成以及化疗增敏等药理作用作一综述     

3种蟑螂密度监测方法与效果分析对比试验

目的验证3种蟑螂密度监测方法之间的实效性、可比性,为蟑螂密度调查提供科学依据.方法采用目前常用的3种蟑螂密度监测方法,在同一现场同步进行对比试验.结果目测法、粘捕法、药激法蟑螂密度检出率分别为19.32%,11.26%,5.86%.3种监测方法结果比较,x2=37.72,P<0.001,差异有显著性意义,其中目测法检出率最高.结论应当结合实际,采用因时、因地制宜的科学监测方法,提高灭蟑效果.     

131I标记抗CD20单克隆抗体在荷瘤裸鼠体内动态分布的实验研究

目的:探讨131I-anti-CD20 McAb经瘤内注射后在荷人Burkitts淋巴瘤细胞系Raji细胞移植瘤裸鼠体内的动态分布。方法:131I标记物的标记采用IODO-GEN碘化标记;注射标记物后第1、3、7、15天将荷瘤裸鼠活杀,定标器测量并计算瘤组织、血液等12种器官或组织的瘤/非瘤(T/NT)比值以及%ID/g值(每克组织摄入注入量的百分数)。结果:131I-anti-CD20 McAb瘤内注射组血液及其他组织器官T/NT比值在给药后第1、3和7天均显著高于腹腔注射组和131I-IgG瘤内注射组(P<0.05);该组肿瘤%ID/g值在给药后第1、3和7天分别为后两组的1.4~17倍和1.7~3.7倍;各非瘤组织和器官的%ID/g值低于后两组。结论:131I-anti-CD20 McAb经瘤内途径给药可以使肿瘤获得最高的放射性药物摄取率和最高的瘤/非瘤组织放射性药物摄取比,为下一步运用该途径进行放射免疫治疗提供了实验依据。     

我国中医药胃肠动力基础实验方法研究进展

中药在胃肠动力动物基础实验研究,目前主要采用胃排空小肠推进率测定法、胃肠道腔外电桥式应力传感器记录法、胃肠激素测定(胃泌素、胃动素、生长抑素等)、免疫组织化学方法、离体体胃肠平滑肌肌条动力测定、胃肠内压描记法.采用最佳的实验研究方法,对中药的研究和认知具有重要的临床意义.     

Bluetongue Virus Infection of Goats: Re-Emerged European Serotype 8 vs. Two Atypical Serotypes

In recent years, numerous atypical Bluetongue virus (BTV) strains have been discovered all around the world. Atypical BTV strains are phylogenetically distinct from the classical BTV serotypes 1–24 and differ in terms of several biological features. For the first time, the atypical strains BTV-25-GER2018 and BTV-33-MNG3/2016 as well as the re-emerged classical strain BTV-8-GER2018 were evaluated comparatively in a pathogenesis study in goats—the natural host of atypical BTV. A substantial number of in-contact animals were included in this study to detect potential contact transmissions of the virus. After infection, EDTA blood, ocular, nasal and oral swab samples as well as serum were collected regularly and were used for virological and serological analyses, respectively. Our study showed differences in the immunological reaction between the two atypical BTV strains (no group-specific antibody detection) and the classical BTV strain BTV-8-GER2018 (group-specific antibody detection). Furthermore, we observed an increase in the total WBC count (neutrophils and lymphocytes) in goats infected with the atypical BTV strains. No horizontal transmission was seen for all three strains. Our study suggests that the atypical BTVs used in the trial differ from classical BTVs in their immunopathogenesis. However, no evidence of direct contact transmission was found.     

马勃的化学成分研究进展及其在创面修复中的应用

马勃Lasiosphaera Calvatia属于真菌类中药,具有消肿、止血、解毒等功效,常以粉末形式外治创伤出血.古籍记载及现代临床发现马勃具有治疗慢性难愈性创面的功效,为深入研究马勃治疗慢性难愈性创面的活性物质基础和药理作用及机制,作者对近年来国内外有关脱皮马勃Lasiosphaera fenzlii Reich....     

医疗失效模式与效应分析在药物基因实验质量管理的应用与实践

目的 通过应用医疗失效模式与效应分析(healthfailure mode and effect analysis,HFMEA),预防药物基因实验的风险事件,提高药物基因实验的操作质量。方法 药学实验室成立失效模式与影响分析(failuremodeandeffectanalysis,FMEA)活动小组,采用头脑风暴法,借助HFMEA模式,识别及分析药物基因实验过程前、中、后可能存在的操作、仪器及环境对药物基因实验质控造成的风险事件,同时制定相对应的解决方案。结果 开展HFMEA活动后,预防与补救了药物基因实验前、中、后的风险事件产生,风险系数值由总分值1 375分降至62.36分,降幅为95.47%(P<0.01);活动小组成员在品管手法、解决问题能力、沟通配合、积极性等方面得到了显著提高。结论 HFMEA活动有助于降低药物基因实验产生风险事件的频次,有效提升实验室的质量管理。     

Study on the Material Properties of Microconcrete by Dynamic Model Test

As an important water conveying structure, the seismic safety of the hydraulic aqueduct has attracted considerable interest. Different from the general bridge structure, the seismic analysis of the aqueduct structure needs to consider its fluid–structure interaction. The existing numerical simulation methods cannot truly reflect the fluid–solid coupling mechanism. Therefore, scholars began to use shaking table tests to study the fluid–structure interaction mechanism. However, the research is immature, and it is mostly focused on the seismic response analysis, and there are few studies on the model test similarity ratio and model material properties. Based on this, in this paper, according to the requirements of the test similarity ratio, the orthogonal experiment was used to explore the influence of barite sand content, water–cement ratio, fine sand ratio, and lime ratio on the mechanical properties of microconcrete. The performance indicators of microconcrete under different mix ratios vary widely, with a minimum variation of 19% and a maximum of 102%. Barite sand has the most significant control effect on the density, and the water–cement ratio has the most significant control effect on the compressive strength and elastic modulus. The density variation range is 2.37–2.81 g/cm³, the cube compressive strength variation range is 18.37–36.94 MPa, and the elastic modulus variation range is 2.11 × 10⁴–3.28 × 10⁴ MPa. This study will provide certain evidence for the similarity ratio design and material selection of the scaled model test of the fluid–solid coupling structure.