Anti-Uterine Fibroid Effect of Standardized Labisia Pumila Var. Alata Extracts In Vitro and in Human Uterine Fibroid Cancer Xenograft Model

Background: Uterine fibroids are a common type of solid tumor presenting in women of reproductive age. There are very few alternative treatment available from conventional treatment involving surgeries. Labisia pumila var. alata or locally known as ‘Kacip Fatimah’ was widely used as traditional medicine in Malaysia. This plant has been used to maintain a healthy female reproductive system. The present study aimed to evaluate anti fibroid potential of L. pumila extracts through in vitro apoptosis activity against uterine leiomyoma cells (SK-UT-1) and in uterine leiomyoma xenograft model. Evaluation of bioactive markers content were also carried out. Methods: Apoptotic induction of the extracts was determined by morphological examination of AO/PI dual staining assay by flourescent microscopy and flow cytometry analysis on Annexin V-FITC/PI stained cells. In vivo study was done in immune-compromised mouse xenograft model. HPLC analysis was employed to quantify marker compounds. Results: Morphological analysis showed L. pumila induced apoptosis in a dose dependent manner against SK-UT-1 cells. In vivo study indicated that L. pumila significantly suppressed the growth of uterine fibroid tumor. All tested extracts contain bioactive marker of gallic acid and cafeic acid. Conclusion: This work provide significant data of the potential of L. pumila in management of uterine fibroids.     

Parasitic leiomyoma in the trocar site after laparoscopic myomectomy: A case report

BACKGROUNDLaparoscopic myomectomy is increasingly used for resecting gynecological tumors. Leiomyomas require morcellation for retrieval from the peritoneal cavity. However, morcellated fragments may implant on the peritoneal cavity during retrieval. These fragments may receive a new blood supply from an adjacent structure and develop into parasitic leiomyomas. Parasitic leiomyomas can occur spontaneously or iatrogenically; however, trocar-site implantation is an iatrogenic complication of laparoscopic uterine surgery. We describe a parasitic leiomyoma in the trocar-site after laparoscopic myomectomy with power morcellation.CASE SUMMARYA 50-year-old woman presented with a palpable abdominal mass without significant medical history. The patient had no related symptoms, such as abdominal pain. Computed tomography findings revealed a well-defined contrast-enhancing mass measuring 2.2 cm, and located on the trocar site of the left abdominal wall. She had undergone laparoscopic removal of uterine fibroids with power morcellation six years ago. The differential diagnosis included endometriosis and neurogenic tumors, such as neurofibroma. The radiologic diagnosis was a desmoid tumor, and surgical excision of the mass on the abdominal wall was successfully performed. The patient recovered from the surgery without complications. Histopathological examination revealed that the specimen resected from the trocar site was a uterine leiomyoma.CONCLUSIONClinicians should consider the risks and benefits of laparoscopic vs laparotomic myomectomy for gynecological tumors. Considerable caution must be exercised for morcellation to avoid excessive tissue fragmentation.     

绦虫感染致急腹症一例

绦虫病是由猪带绦虫、牛带绦虫和亚洲带绦虫寄生于人体肠道所引起的食源性人兽共患寄生虫病。人类是唯一的最终宿主。该文报道一例绦虫感染致急腹症患者的诊治经过。该例28岁男性患者主要表现为腹痛,入院后行血常规、生化、影像学等检查,阑尾炎表现不典型,考虑其他原因的急腹症,经禁饮、禁食、灌肠、抗炎、补液等保守治疗后,患者恢复排便排...     

100例子宫平滑肌瘤的临床病理分析和超微结构观察

本文对100例子宫平滑肌瘤作了前瞻性研究。本组病人年龄25～55岁,87%为中年(35～50岁)妇女。对伴有肌瘤的内膜和卵巢的周期性组织学变化进行了比较。结果显示:≤40岁的内膜,同步95.7%,不同步4.3%;>40岁的内膜,同步79.7%,不同步13.6%,增生过长和息肉各2例占6.7%。不支持肌瘤常与内膜增生过长共存的观点。100例采用HE、组织化学技术和8例用电镜对比观察了肌瘤和正常肌层。结果显示,肌瘤细胞和肌层SMC的彤态学基本相似。电镜并显示SMC可产生胶原原纤维和胶原纤维。基于这个观察,我们支持肌瘤可能源自肌层SMC的观点。     

结节性肝寄生虫感染的声像图特征

目的　探讨结节性肝寄生虫感染性病变的二维及彩色多普勒特征及病理组织学基础。方法分析了经超声引导下穿刺活检病理证实的 11例患者 16个直径 3cm以下的结节性肝寄生虫病变的二维及彩色多普勒声像图特征 ,并与病理结果进行对照。结果　 11例患者共 16个结节 ,其中单发结节 7例 ,2个结节 3例 ,3个结节 1例 ;病变形态多不规则 ,5例呈较均匀低回声 ,6例呈混合性回声 ,低回声中混有点状高回声 ,肿块一般边界清楚 ,但无包膜或包膜不完整 ,周边无低回声暗环。CDFI显示病灶内血流不丰富 ,11例中仅 1例于病灶内引出低速动脉血流 (12cm /s)。 6例低回声为主的混合性回声结节的活检标本镜下为以嗜酸性白细胞为主的大量炎细胞浸润 ,3例伴组织凝固性坏死 ,2例于坏死组织中找到夏科 -莱登结晶。 5例低回声结节均为较均匀的组织凝固性坏死。结论　肝脏 <3cm结节性寄生虫感染一般表现为肝内的实性病变 ,以不均匀低回声或低回声多见 ,边界多不规则 ,无完整包膜 ,CDFI检查肿块内多无血流信号。上述特征有利于与肝恶性病变区别 ,鉴别诊断困难者超声引导下穿刺活检是确诊的必要手段     

超选择子宫动脉栓塞治疗症状性子宫肌瘤的临床研究

目的:研究超选择性子宫动脉栓塞治疗子宫肌瘤的临床效果。方法:采用Seldinger法穿刺并超选择双侧子宫动脉PVA颗粒加明胶海绵栓塞治疗18例子宫肌瘤患者。结果:经双侧子宫动脉栓塞后6个月随访结果表明月经周期和经量恢复正常,疼痛和贫血等明显改善,肌瘤和子宫体积明显缩小。结论:超选择性子宫动脉栓塞治疗子宫肌瘤是一种创伤小、临床和社会效果好、安全、有效的新方法。     

PVA与LPE子宫动脉栓塞治疗子宫肌瘤合并子宫腺肌病的疗效观察

目的 对比评价聚乙烯醇颗粒(PVA)与平阳霉素碘油乳剂(PLE)两种材料在行子宫动脉栓塞治疗子宫肌瘤合并子宫腺肌病的临床疗效.方法 对28例根据临床表现、超声及DSA造影诊断为子宫肌瘤合并子宫腺肌病的患者随机分为A、B两组,A组14人应用PVA行子宫动脉栓塞术(UAE);B组14人应用PLE行栓塞术.28例患者均应用微导管行超选择性子宫动脉插管并注射栓塞材料.术后随访6～30个月观察病人的痛经程度,月经量及子宫病灶的体积变化.结果 栓塞技术成功率100%.A组8例、B组10例术后3月痛经消失;A组6例、B组4例治疗后症状明显缓解.术后5个月痛经消失.A、B组患者术后6个月子宫体积平均缩小41.83%,43.59%.术后6个月A组、B组肌瘤体积平均缩小分别为51.63%,52.9%.结论 PVA与PLE两种材料在子宫动脉栓塞治疗子宫肌瘤合并子宫腺肌病的临床疗效对比观察中无明显差别,无统计学意义(P＞0.05).     

血管造影对小肠平滑肌肿瘤的临床应用价值

目的：分析小肠平滑肌肿瘤的血管造影的Ｘ线表现,探讨血管造对小肠平滑肌肿瘤的临床应用价值。材料和方法：应用选择性肠系膜上动脉造影,回顾性分析了１１例小肠平滑肌肿瘤的血管造影征象。结果：血管造影主要表现为：（１）肿瘤的供血动脉增组;（２）动脉期显示一团病理血管;（３）可有血管池;（４）肿瘤染色;（５）可出现静脉早显。结论：（１）血管造影对小肠平滑肌肿瘤的诊断价值优于钡剂造影和内镜检查,可作为本病的首冼     

B型超声普通探头引导经皮肝穿刺无水酒精注射治疗非寄生虫性肝囊肿

１９８９年４月至１９９７年１０月我院采用超声普通探头引导下肝穿刺抽吸囊液后注射无水酒精治疗非寄生虫性肝囊肿１０２例，共１４７个囊肿病灶，其中多发性肝囊肿７２例，单发性肝囊肿３０例，最大囊肿直径２０．０ｃｍ，平均每例治疗３～４次，一次抽液量最多为１３７０ｍｌ，累计抽液量最多为４２７０ｍｌ，治疗结果：６９个囊肿治愈（占４６．９％），７８个囊肿显著缩小（占５３．１％），共治疗５０６次，未发生严重并发症。本法具有适应症广，创伤小，副作用少，疗效好，经济负担少，患者易于接受等优点