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Nandita M deSouza Clare M Tempany 《International journal of cancer. Journal international du cancer》2019,144(3):422-430
Recognition of <3 metastases in <2 organs, particularly in cancers with a known predisposition to oligometastatic disease (OMD) (colorectal, prostate, renal, sarcoma and lung), offers the opportunity to focally treat the lesions identified and confers a survival advantage. The reliability with which OMD is identified depends on the sensitivity of the imaging technique used for detection and may be predicted from phenotypic and genetic factors of the primary tumour, which determine metastatic risk. Whole-body or organ-specific imaging to identify oligometastases requires optimization to achieve maximal sensitivity. Metastatic lesions at multiple locations may require a variety of imaging modalities for best visualisation because the optimal image contrast is determined by tumour biology. Newer imaging techniques used for this purpose require validation. Additionally, rationalisation of imaging strategies is needed, particularly with regard to timing of imaging and follow-up studies. This article reviews the current evidence for the use of imaging for recognising OMD and proposes a risk-based roadmap for identifying patients with true OMD, or at risk of metastatic disease likely to be OM. 相似文献
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目的:探讨增殖性肾小球肾炎大鼠动物模型中系膜细胞α-平滑肌肌动蛋白(α-SMA)表达与细胞表型的关系。方法:应用单克隆抗体1-22-3(MoAb1-22-3)及Habu蛇毒素分别诱导大鼠增殖性肾小球肾炎动物模型。用免疫组化技术并结合计算机图像分析,检测两个不同诱因引起模型的各个阶段的系膜细胞内α-平滑肌肌动蛋白,SMeb的表达程度,系膜细胞增殖和细胞外基质(ECM)分泌的情况。结果:在MoAb1-22-3模型,随着系膜细胞增殖程度的加理,ECM积聚增多,α-SMA的表达程度也增加,三者平行变化并具有高度相关性,而Habu模型则系膜细胞增殖和ECM增加的同时α-SMA,SMemb表达不增加。结论:α-SMA不是系膜细胞被激活并处于增殖/分泌表型唯一标志。 相似文献
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转化人关节软骨细胞的Ⅱ型胶原表型诱导 总被引:1,自引:0,他引:1
目的 用离心管聚集体培养(Aggregate culture)诱导转化人关节细胞的Ⅱ型胶原。方法 将体外长期培养的第30,40,50代转化软骨细胞消化后进行离心管培养,比较细胞在单层培养,离心管聚集体培养,以及在普通培养基,RAI诱导培养基下[2型骨形态发生蛋白(BMP-2)+抗坏血酸盐(Ascorbate) 胰岛素(insulin)],Ⅰ,Ⅱ,Ⅲ型胶原的表达和胞外基质发生情况。结果 在单层培养条件下,转化软骨细胞只表达Ⅰ型胶原,而在BAI诱导培养基及离心管聚集体培养下转化软有细胞表达的Ⅱ型胶原和大量胞外基质。结论 离心管聚集体培养是诱导转化软骨细胞Ⅱ型胶原的有效方式。 相似文献
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采集23名产妇的脐带血和8名正常成人的外周血制备LAK细胞,随机分为4组,在培养第3,5,7,14天,分别利用MTT法和^125I-UdR释放法对4组LAK细胞的增殖力和杀伤活性进行了测定,同时用细胞毒方法测定培养7天的LAK细胞的免疫表型。结果发现,在不同培养时间,脐血来源的LAK细胞增殖力显著高于成人血LAK细胞(P〈0.05或P〈0.01),成人血清组,脐血血清组,红细胞组LAK细胞增殖力有 相似文献
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Gabriele Masi Giulio Perugi Cristina Toni Stefania Millepiedi Maria Mucci Nicoletta Bertini Hagop S Akiskal 《Neuropsychopharmacology》2006,59(7):603-610
BACKGROUND: Recent research has addressed the issue of subtyping juvenile bipolar disorder (JBD). Accordingly, we set out to find out, in a naturalistic sample of bipolar children and adolescents with mania and mixed mania, whether the most useful subtyping should be based on clinical features (elated vs. irritable) or course (episodic vs. chronic). METHODS: We studied 136 patients, 81 male patients (59.6%) and 55 female patients (40.4%), mean age 13.5 +/- 2.9 years, meeting the DSM-IV diagnosis of bipolar disorder, assessed by a structured clinical interview (Schedule for Affective Disorders and Schizophrenia for School-Age Children-Present and Lifetime Version [K-SADS-PL]). RESULTS: Regarding course, 77 patients (56.6%) had an episodic course and 59 patients (43.4%) had a chronic course. Patients with chronic course were significantly younger, had an earlier onset of JBD, and presented a more frequent comorbidity with disruptive behavior disorders. According to the prevalent mood disturbance, 75 patients (55.1%) showed an elated and 61 patients (44.9%) showed an irritable mood. Elated mood was more frequent in patients with episodic course, whereas irritable mood was more frequent in the patients with chronic course. CONCLUSIONS: These findings suggest that chronic versus episodic course may be a putative differential feature. Further validation of such a distinction would require prospective studies, temperament evaluation, gender and neurobiologic approaches, and differential psychopharmacologic assignment and response. 相似文献
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胰岛素对大鼠血管平滑肌细胞表型转化的影响 总被引:2,自引:0,他引:2
目的:探讨胰岛素对体外培养的血管平滑肌细胞(vascular smooth muscle cell,VSMC)表型转化的影响.方法:用酶消化法分离大鼠VSMC,采用免疫组织化学法检测胰岛素作用后VSMC肌动蛋白(α-SM actin),RT-PCR 检测胰岛素作用后VSMC中bFGF、TGF-β、PDGF、matrix Gla和OPN各目的基因mRNA相对表达水平.结果:胰岛素组VSMC的3H-TdR掺入值比对照组升高47%(P<0.01), VSMC α- SM actin免疫组化结果显示对照组的α-SM actin比胰岛素组染色深.而matrix Gla和OPN在培养的VSMC中mRNA的表达量,胰岛素组明显高于对照组(P<0.05),同时bFGF、TGF-β、PDGF的表达胰岛素组也明显高于对照组(P<0.05).并可明显见到细胞骨架F-actin、G-actin重新分布.结论:在合成表型的matrix Gla和OPN表达量明显高于收缩表型,而合成表型的α- SM actin表达量明显低于收缩表型.提示胰岛素对VSMC的表型转化起了一定作用.胰岛素在促进了VSMC增殖的同时,伴有VSMC由收缩型转变为合成型及细胞骨架F-actin、G-actin重新分布. 相似文献
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Berend H. F. Sombekke Willemina M. Molenaar Anthonie J. van Essen Coen J. F. Schoots 《Fetal and pediatric pathology》1994,14(2):277-285
Congenital muscular dystrophy (CMD) comprises a heterogeneous group of muscle disorders. We report on two stillborn sibs with early lethal CMD and a prematurely born boy who died within minutes after birth. The pregnancies were complicated by polyhydramnios. All presented with arthrogryposis multiplex congenita, severe muscle wasting, lung hypoplasia, and hydrops. The muscle biopsies showed fibrosis, variation in fiber size, and extensive fat replacement compatible with muscular dystrophy. Fatal CMD seems to be distinct from CMD with survival after birth and is probably autosomal recessively inherited. 相似文献
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A monoclonal antibody has been raised against a surface membrane antigen present on leukemic myeloblasts. In 52 consecutive patients with acute myeloid leukemia treated in a standardized fashion with intensive chemotherapy the immunologic subclass with respect to this antigen was correlated to the clinical outcome. We found the expression of this antigen to predict a poor prognosis, when measured as survival of CR-patients and as survival after 1st relapse. 相似文献