The gerbil is a rodent considered a good model for studies of prostatic morphophysiology under different experimental conditions. Studies involving castration and steroidal blockers of aged gerbils showed that the glandular epithelium persists after long-term therapy, preventing the organ atrophy. Thus, the objective of this study was to evaluate the phenotypic characteristics and behavior of prostatic epithelial cells that remained after different periods of hormone ablation in aged gerbils. The identification of elements that influenced the survival of this cell type was performed by morphometric, nuclear phenotypes, ultrastructural and immune histochemical analysis. The most significant responses to treatment, by analyzing morphometric features, were observed during the first three time points (day 1, day 3, and day 7), after which there appeared to be an adjustment of the gland to the hormone ablation. All treatments led to changes in the state of chromatin condensation, DNA methylation pattern and phenotypic changes indicated cell senescence. Additionally, an increase in the basal cells seemed to guarantee self-renewal properties to the epithelium. These data indicate that changes occur at many levels, including gene expression and nuclear architecture in the epithelial cells, when aging and steroidal blockade are associated. These aspects are important when considering castration-resistant prostate cancer, a malignant tumor posing difficult therapeutic intervention. 相似文献
The regulation of glucose, lipid metabolism and immunoreactivities of insulin and glucagon peptides by delta-9-tetrahydrocannabinol (Δ9-THC) in diabetes were examined in an experimental rat model. Male Sprague-Dawley rats were divided into four groups: (1) control, (2) Δ9-THC treated, (3) diabetic, and (4) diabetic + Δ9-THC. The type 2 diabetic rat model was established by intraperitoneal (i.p.) injection of nicotinamide (85 mg/kg body weight) followed after 15 min by i.p. injection of streptozotocin (STZ) at 65 mg/kg of body weight. Δ9-THC and Δ9-THC treated diabetic groups received 3 mg/kg/day of Δ9-THC for 7 days. The immunolocalization of insulin and glucagon peptides was investigated in the pancreas using a streptavidin–biotin–peroxidase technique. High density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL), very low density lipoprotein cholesterol (VLDL), triglycerides (TG), total cholesterol (TC) and total protein (TP) levels were measured in serum. Total islet area percent of insulin immunoreactive cells slightly changed in diabetic + Δ9-THC rats compared to diabetic animals. However, the area percent of glucagon immunoreactive cells showed a decrease in diabetic + Δ9-THC rats compared to that of diabetic animals alone. Serum TC, HDL and LDL levels of diabetes + Δ9-THC group showed a decrease compared to the diabetic group. These results indicate that Δ9-THC may serve a protective role against hyperlipidemia and hyperglycemia in diabetic rats. 相似文献
The interruption of supraspinal input to the spinal cord leads to motor dysfunction and the development of spasticity. Clinical studies have shown that Baclofen (a GABAB agonist), while effective in modulating spasticity is associated with side-effects and the development of tolerance. The aim of the present study was to assess if discontinued Baclofen treatment and its repeated application leads antispasticity effects, and whether such changes affect neuronal nitric oxide synthase (nNOS) in the brainstem, nNOS and parvalbumin (PV) in lumbar α-motoneurons and glial fibrillary acidic protein in the ventral horn of the spinal cord. Adult male Wistar rats were exposed to Th9 spinal cord transection. Baclofen (30 mg/b.w.) diluted in drinking water, was administered for 6 days, starting at week 1 after injury and then repeated till week 4 after injury. The behavior of the animals was tested (tail-flick test, BBB locomotor score) from 1 to 8 weeks. Our results clearly indicate the role of nitric oxide, produced by nNOS in the initiation and the maintenance of spasticity states 1, 6 and 8 weeks after spinal trauma. A considerable decrease of nNOS staining after Baclofen treatment correlates with improvement of motor dysfunction. The findings also show that parvalbumin and astrocytes participate in the regulation of ion concentrations in the sub-acute phase after the injury. 相似文献
A fused aromatic furan‐substituted diketopyrrolopyrrole and novel diphenylfumaronitrile conjugated building blocks are used for the synthesis of an alternating copolymer ( DPFN‐DPPF ) via Suzuki polycondensation. In this paper, the first attempt to use the diphenylfumaronitrile building block for the synthesis of conjugated polymer is described. The number‐average and weight‐average molecular weights calculated for DPFN‐DPPF are 20 661 and 66 346 g mol?1, respectively. The optical bandgap calculated for DPFN‐DPPF is 1.53 eV whereas the highest occupied molecular orbital (HOMO) value calculated by photoelectron spectroscopy in air (PESA) is 5.50 eV. The calculated HOMO value is lower, which is suitable for stable organic electronic devices. DPFN‐DPPF polymer is used as an active layer in bottom‐contact bottom‐gate organic thin‐film transistor devices and the thin film exhibits a hole mobility of 0.20 cm2 V?1 s?1 in air.
The binding of tumour necrosis factor α (TNFα) to cell surface receptors engages multiple signal transduction pathways, including three groups of mitogen-activated protein (MAP) kinases: extracellular-signal-regulated kinases (ERKs); the cJun NH2-terminal kinases (JNKs); and the p38 MAP kinases. These MAP kinase signalling pathways induce a secondary response by increasing the expression of several inflammatory cytokines (including TNFα) that contribute to the biological activity of TNFα. MAP kinases therefore function both upstream and down-stream of signalling by TNFα receptors. Here we review mechanisms that mediate these actions of MAP kinases during the response to TNFα. 相似文献
目的:探讨重症肺炎患者肺泡灌洗液(BALF)中微小核糖核酸-127-5p(miRNA-127-5p)的表达水平及临床价值。方法:选取我院2017年1月至2019年5月重症肺炎患者102例进行分析。所有患者第1天行纤维支气管镜检查;在病程第4、7天经纤维支气管进行支气管肺泡灌洗(BAL);选择右肺中叶或左上叶舌段,注入0.9%氯化钠液之后,使用合适的负压(100 mm Hg,1 mm Hg=0.133 k Pa)吸取BALF;应用聚合酶链式反应(PCR)法检测miR-127-5p表达量;采用酶联免疫吸附实验(ELISA)法对BALF中IL-6、IL-8及IL-10水平进行测定。结果:动态监测两组患者BALF中的miRNA-127-5p表达量以及IL-6、IL-8及IL-10水平;治疗第1天,两组的miRNA-127-5p表达量以及IL-6、IL-8及IL-10水平,差异无统计学意义(P>0.05);治疗第4天,预后良好组的miRNA-127-5p表达量显著高于预后不良组,差异有统计学意义(P<0.05);治疗第4天,预后良好组的IL-8,IL-10水平显著低于预后不良组,差异有统计学意义(P<0.05);治疗第7天,预后良好组的miRNA-127-5p表达量显著高于预后不良组,差异有统计学意义(P<0.05);治疗第7天,预后良好组的IL-8,IL-10水平显著低于预后不良组,差异有统计学意义(P<0.05);经治疗,两组的miRNA-127-5p表达量逐渐升高,IL-8,IL-10水平逐渐降低。miRNA-127-5p表达量在预测重症肺炎患者预后情况的AUC曲线下面积为(敏感度=88.6%,特异性=70.9%)。结论:肺泡灌洗液中miRNA-127-5p表达量有望成为一种判断重症肺炎患者的预后的新型生物标志物。 相似文献