花刺参黏多糖对胎牛血清诱导的血管平滑肌细胞增殖和凋亡的影响

目的观察花刺参黏多糖对10%胎牛血清(FBS)诱导的大鼠血管平滑肌细胞增殖和凋亡的影响,以探讨花刺参黏多糖抗动脉粥样硬化、预防经皮腔内冠状动脉成形术后再狭窄的可能机制。方法组织块贴片法体外原代培养大鼠血管平滑肌细胞,细胞增殖采用MTT、流式细胞周期分析;流式细胞术、TUNEL实验方法观察细胞凋亡。结果10?S刺激可促进血管平滑肌细胞增殖、抑制其凋亡,而花刺参黏多糖(5~20g.L-1)有浓度依赖性地逆转作用,MTT吸光度(A值)由0.484±0.034减少到0.369±0.019(P<0.01),流式细胞分析示G0/G1期细胞比例由54.7±2.7%增加到75.8±1.8%(P<0.01);流式细胞分析示细胞凋亡率由2.84±1.44%增加到13.51±2.75%(P<0.01);TUNEL结果显示凋亡细胞比例由2.90±1.05%增加为8.60±1.13%(P<0.01)。结论花刺参黏多糖可抑制10?S诱导的血管平滑肌细胞增殖,促进其细胞凋亡,这可能对延缓动脉粥样硬化发生发展以及预防经皮腔内冠状动脉成形术后再狭窄发生起到一定作用。     

赤软骨黏多糖对家兔血脂水平和凝血时间的影响

目的研究赤软骨黏多糖的降血脂和抗凝血活性。方法观察赤软骨黏多糖对高脂家兔血脂的调节作用和正常家兔全血凝血时间的影响。结果赤软骨黏多糖能显著降低高脂家兔血清总胆固醇(TC)、血清甘油三酯(TG)和血清低密度脂蛋白(LDL-C)含量,提高血清高密度脂蛋白胆固醇(HDL-C)含量,对正常血脂家兔的胆固醇无明显影响,能显著延长家兔全血的凝血时间。结论赤软骨黏多糖具有较强的降血脂和抗凝血活性。     

多磺酸基粘多糖乳膏治疗儿童冻疮疗效观察

目的 探讨多磺酸基粘多糖乳膏治疗儿童冻疮的临床疗效及安全性.方法 60例儿童冻疮患者随机分为对照组和治疗组,对照组给予复方醋酸地塞米松乳膏,治疗组给予多磺酸基粘多糖乳膏,治疗2周后比较临床疗效.结果 治疗组有效率显著高于对照组（93.33％和S71.67％）,差异有统计学意义（P＜0.05）.对照组和治疗组患者临床症状及体征评分均较治疗前显著下降,差异有统计学意史（P＜0.05）;且治疗组下降更为明显,差异有统计学意义（P＜0.05）.结论 多磺酸基粘多糖乳膏可显著改善患儿的临床症状及体征,治疗儿童冻疮安全有效.     

刺参粘多糖及考的松对小鼠移植瘤S180的作用

刺参粘多糖(SJAMP)同考的松合用对于小鼠S180实体瘤的生长有明显的抑制作用,该作用与肝素合用考的松的作用相似。由于肝素合用考的松具有抑制肿瘤血管生成的作用,而SJAMP在化学结构和理化特性方面同肝素相似。所以本实验结果提示,SJAMP同考的松合用可能具有抑制肿瘤血管生成的作用。     

多磺酸黏多糖乳膏治疗PICC所致静脉炎的笛床疗效观察

目的探讨多磺酸黏多糖乳膏治疗PICC并发静脉炎的效果。方法将并发静脉炎患者分为实验组和对照组。实验组用多磺酸黏多糖乳膏治疗,对照组利用传统方法进行治疗,对两组患者的临床症状、消失时间、PICC管留置时间进行比较。结果试验组患者临床消失时间短,留置时间明显长于对照组,患者整体满意度高。结论多磺酸黏多糖乳膏治疗PICC并发静脉炎效果肯定,值得临床推广应用。     

骨组织成分与Masson三色染色反应的关系分析

为研究骨组织主要成分对其Masson三色染色结果的影响,取大鼠股骨上端及尾部中段标本,经Bouin固定液固定,甲酸脱钙后,石蜡切片,对照片染Masson三色及Safranin-0染色,实验片经过0．5％透明质酸酶消化,2mol.L^-1氯化钙溶液抽提粘多糖后,染色同对照片,另取人脐带标本,经Bouin液固定,石蜡切片,实验片经透明质酸酶消化后,做Safranini-o染色,对照片不经透明质酸消化,其余处理及染色同实验片,结果示Safranin-o对组织中粘多糖染鲜红色,经透明质酸酶消化及氯化钙溶液抽提,其着染减退或消失。Masson三色染色,软骨胶原呈绿色,真皮,骨及钙化软骨中胶原呈红－绿相间,骨质越成熟,红染越多,经透明质酸酶消化及氯化钙溶液处理染色无改变,表明骨组织中胶原的成熟程度影响Masson三色的染色反应,胶原越成熟红染越多,胶原类型,粘多糖及钙化成分不影响骨组织对Masson三色的染色反应。     

Chondroitinase: A promising therapeutic enzyme

Abstract

Even after 20 years of granting orphan status for chondroitinase by US FDA, there is no visible outcome in terms of clinical use. The reasons are many. One of them could be lack of awareness regarding the biological application of the enzyme. The biological activity of chondroitinase is due to its ability to act on chondroitin sulfate proteoglycans (CSPGs). CSPGs are needed for normal functioning of the body. An increase or decrease in the level of CSPGs results in various pathological conditions. Chondroitinase is useful in conditions where there is an increase in the level of CSPGs, namely spinal cord injury, vitreous attachment and cancer. Over the last decade, various animal studies showed that chondroitinase could be a good drug candidate. Research focusing on developing a suitable carrier system for delivering chondroitinase needs to be carried out so that pharmacological activity observed in vitro and preclinical studies could be translated to clinical use. Further studies on distribution of chondroitinase as well need to be focused so that chondroitinase with desired attributes could be discovered. The present review article discusses about various biological applications of chondroitinase, drug delivery systems to deliver the enzyme and distribution of chondroitinase among microbes.     

热敷加喜疗妥乳膏治疗肌内注射后臀部硬结的效果观察

目的观察热敷加喜疗妥乳膏治疗肌内注射后硬结的效果。方法将150例肌内注射后臀部发生硬结的患者随机分为实验组与对照组各75例。对照组采用水温50-60℃浸透的纱布热敷肌内注射硬结,每次20 min,3次/d;实验组采用热敷肌内注射硬结部位20 min,再用喜疗妥乳膏在硬结部位外涂3-5 min,3次/d。观察两组臀部肌内注射后硬结直径的大小,并记录肌内硬结软化程度的变化。结果实验组有效75例,治疗有效率为100.0%;对照组有效63例,治疗有效率为84.0%,两组治疗效果比较,χ2=15.81,P〈0.01,差异有统计学意义。结论热敷加喜疗妥乳膏能有效地消除肌内注射后引起的臀部硬结,具有操作简单、方便、节省护理时间等优点,值得临床推广应用。     

Recessively inherited,late onset spondylar dysplasia and peripheral corneal opacity with anomalies in urinary mucopolysaccharides: A possible error of chondroitin-6-sulfate synthesis

Two male and two female sibs with an unusual form of spondyloepiphyseal dysplasia were reported. The main clinical features were low stature, moderate shortness of trunk and neck, abnormal span: height ratio, low-normal UBS: LBS ratio, and peripheral corneal punctate opacities only seen by the slitlamp. Normal mental status was present. Typical metachromatic granules were not seen either in bone-marrow cells or in peripheral blood cells. The X-ray picture showed spondylar and pelvic dysplasias. Qualitative rather than quantitative anomalies were shown in the urinary mucopolysaccharides, mostly involving chondroitin-6-sulfate. The genetic data are consistent with autosomal recessive inheritance.