肥厚性瘢痕组织形态与胶原降解关系的实验研究

本文观察了肥厚性瘢痕的组织形态，探讨了它们与胶原降解的关系，结果发现肥厚性瘢痕     

Case of diabetic scleredema: diagnostic value of magnetic resonance imaging

We report the case of a patient with a 20-year history of diabetes mellitus type 2 who developed sclerotic skin lesions on his neck and upper back. Physical and histological findings were compatible with diabetic scleredema. T(2)-weighted magnetic resonance imaging (MRI) revealed diffuse thickening of the dermis and subcutaneous tissue, with hyperintense signals. The MRI findings in the dermis corresponded with the accumulation of collagen tissue with deposition of glycosaminoglycans.     

甲基莲心碱对移植至裸鼠的增生性瘢痕胶原和酸性粘多糖合成的影响

目的 探讨甲基莲心碱(Neferine，Nef)对移植至裸鼠的人增生性瘢痕的作用。方法 建立人增生性瘢痕裸鼠动物模型，将瘢痕组织块植入18只裸鼠皮下，术后第24d组织成活后，随机分为3组：①Nef治疗组(A组)；②稀盐酸对照组(Nef溶媒0．03M HCl：NS＝1：9)(B组)；③空白对照组(C组)。A、B组每只每2d分别局部注射Nef l00μl(1．2mg／ml)和稀盐酸100μl。两周后，用光、电镜观察组织和细胞形态学变化，测量植入物的体积，用全自动图像分析仪测量组织中胶原和酸性粘多糖含量。结果 A组植入物体积缩小，与B、C组比较，差异有非常显著性意义(P＜0．001)；胶原排列较整齐；I型胶原减少，Ⅲ型胶原增加；胶原和酸性粘多糖含量显著降低(P＜0．001)。结论 Nef对移植至裸鼠的人增生性瘢痕有减小体积，改变Ⅰ、Ⅲ型胶原成分，降低胶原和酸性粘多糖含量的作用。表明Nef对移植至裸鼠的增生性瘢痕有一定的治疗作用。     

消瘀片治疗新型血小板聚集功能缺陷症的临床研究

本研究采用中药消瘀片治疗新型血小板聚集功能缺陷症７２例，并与维生素Ｃ、Ｐ加安络血组６５例作对照，进行临床症状和血小板聚集功能的疗效比较。结果：显效率：中药组为８７．５％，对照组为３５．４％；两组小血小板聚集缺陷恢复率分别为９１．８％与１１．８％；停药６个月后随访的复发率分别为３０．８％与８２．１％。两组间的差异均非常显著（Ｐ＜０．０１）。结果表明：消瘀片有调节止血功能和血小板聚集功能，值得临床推广     

67Ga-Binding substances in stomach,small intestine,pancreas, and muscle

Normal male rats were injected with either gallium citrate Ga 67 or sodium sulfate S 35. After 24 h, the stomach, small intestine, pancreas, and muscle were excised and homogenized. After the removal of the nuclear fraction, each of these homogenates was digested with protease. After digestion, the supernatants of the reaction mixtures were applied to a Sephadex-G-100 column. The radioactivity was eluted with buffer solution. The resultant eluates were analyzed for radioactivity and the levels of proteins, uronic acids, and sialic acids. In all four organs, sizable amounts of ⁶⁷Ga were bound to sulfated acid mucopolysaccharides with molecular masses of about 10,000 daltons and to sulfated acid mucopolysaccharides, a species whose molecular masses exceed 40,000 daltons. In the stomach, large amounts of ⁶⁷Ga were bound to sulfated acid mucopolysaccharides with molecular masses of about 10,000 daltons. from these results, it is obvious that the main ⁶⁷Ga-binding substances in these four organs are sulfated acid mucopolysaccharides, and that these acid mucopolysaccharides play the most important role in the concentration of ⁶⁷Ga in these organs.     

MUCOLIPIDOSIS II (I-CELL DISEASE) A Clinical and Biochemical Study

The diagnosis of mucolipidosis I1 (I-cell disease) was made in a patient with a Hurler like appearance but only borderline muco-polysacchariduria. The cultured fibroblasts of high doses of prednison, which resulted in a further increase in the enzyme activity. On the ultrastructural level there was the storage of polymorphous material in the liver and in the peripheral nerve. Vacuolated cells were present in the peripheral leucocytes and in the bone marrow. These findings indicate that the lysosmal enzyme depletion in the cells in the patient could be similar to that found in cultured cells. However, the clinical picture could also be caused by the excess activity of lysosomal enzymes in the extracellular fluids which are able to degrade the matrix substance of the connective tissues and could mimic a mucopolysaccharidosis.     

玉足海参酸性多糖的研究

干燥玉足海参体壁经碱法消化、钾盐和季铵盐分级,可获得两种含岩藻糖的酸性多糖HL-P和HL-S。两者经电泳、凝胶过滤及超离心分析均证明为纯一成分。理化分析表明,HL-S系聚岩藻糖硫酸酯,此类多糖在多种海参中存在;HL-P系一硫酸粘多糖,其组成中,氨基半乳糖:葡萄糖醛酸:岩藻糖:硫酸基的分子比值为1:0.94:0.84:3.60。本文报告了两种多糖的比旋度、特性粘度、沉降系数及分子量。生物活性测定表明,HL-P对牛凝血酶有较强抑制作用(体外),同时抑制多种小鼠移植瘤的生长。     

远红外线治疗仪联合多磺酸黏多糖乳膏在自体动静脉内瘘狭窄术后的应用

目的:观察远红外线治疗仪联合多磺酸黏多糖乳膏在自体动静脉内瘘狭窄术后的应用效果。方法:选取行自体动静脉内瘘狭窄术患者115例,简单随机法分为对照组57例和观察组58例。对照组术后内瘘处涂抹多磺酸黏多糖乳膏,而观察组在内瘘处涂抹多磺酸黏多糖乳膏基础上采用远红外线治疗仪进行红外线照射。比较两组术后并发症、内瘘及术后半年内血管通路通畅情况,比较两组炎症因子的差异。结果:观察组和对照组累计并发症分别为2例和8例。治疗前,两组炎症因子水平比较无显著差异（P>0.05）,治疗后,两组炎症因子水平均下降,观察组上述指标下降程度大于对照组（P<0.05）。治疗前,两组内瘘情况比较,差异无统计学意义（P>0.05）,治疗后,两组内瘘血管内径增粗、血流量升高,内瘘血管峰值流速下降,观察组内瘘血管内径、血流量及血管峰值流速改善程度大于对照组（P<0.05）。两组术后1、3、6个月时血管通路通畅率比较无显著差异（P>0.05）。结论:远红外线治疗仪联合多磺酸黏多糖乳膏可减少自体动静脉内瘘狭窄术后应激炎症反应,改善内瘘血管内径和血流量,降低并发症。     

A histochemical study of bone marrow hypoplasia in anorexia nervosa

Summary The bone marrow in patients with anorexia nervosa is commonly hypoplastic with transformation of marrow fat. The normal fat cells which appear clear and open in the marrow are surrounded by an amorphous, gelatinous material, thought to represent an increase in the ordinary acid mucopolysaccharide ground substance of the bone marrow. Since this lesion has a similar appearance grossly and microscopically to the lesion of serous fat atrophy found in cachectic patients, we have compared the histochemical properties of this amorphous material in a bone marrow from a patient with anorexia nervosa and from cachectic patients with epicardial serous fat atrophy and with the background substance in hypoplastic marrows. Both this fat-associated deposition in the bone marrow and serous fat atrophy were found to be predominantly a hyaluronic acid mucopolysaccharide. In contrast, the background substance contained a less acid mucopolysaccharide. The increase in bone marrow acid mucopolysaccharide in anorexia nervosa may represent a serous fat atrophy change rather than an increase in ground substance.     

多磺酸粘多糖乳膏预防奥沙利铂所致化学性静脉炎疗效观察

目的:探讨多磺酸粘多糖乳膏治疗化学性静脉炎的疗效。方法:将80例化学性静脉炎患者随机分为两组,治疗组应用多磺酸粘多糖乳膏外涂,对照组采用50%硫酸镁溶液湿敷,7 d后观察两组患者疗效并比较有效率。结果:治疗组有效率明显高于对照组,两组比较差异有统计学意义(P<0.05)。结论:使用多磺酸粘多糖乳膏预防奥沙利铂所致化学性静脉炎有效率高,方便实用,值得临床推广。