妊娠期糖尿病患者血清miR-149水平与胰岛素抵抗的关系

【摘要】 目的 探讨妊娠期糖尿病（GDM）患者血清miR-149水平与胰岛素抵抗的关系。 方法 选取2017年1月~2018年8月陕西省人民医院收治的63例妊娠期糖尿病患者设为观察组,选择本院同期产检健康妊娠产妇63例设为对照组,用自动糖化血红蛋白检测仪测定糖化血红蛋白（HbA1c）,用生化分析仪检测受试者餐前空腹血糖(FPG),用免疫测定分析仪检测血清空腹胰岛素（FINS）,计算胰岛素抵抗指数（HOMA IR）=FPG×FINS/22.5。采用实时荧光定量PCR（qRT-PCR）法检测所有研究对象血清miR-149表达水平。用Pearson分析miR-149与血糖、胰岛素等指标的相关性。对影响胰岛素抵抗的相关因素进行Logistic回归分析。 结果 与对照组相比,观察组孕妇HOMA-IR、FPG、FINS、HbA1C水平、miR-149水平较高（P＜0.05）;Pearson法分析显示观察组孕妇血清miR-149水平与FPG、FINS、HOMA-IR呈正相关（P＜0.05）,与HbA1C无相关性（P＞0.05）;多因素Logistic回归分析显示血清miR-149水平升高是影响胰岛素抵抗的危险因素。 结论 妊娠期糖尿病患者血清miR-149水平上调,可能与妊娠期糖尿病孕妇胰岛素抵抗有关。     

Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus

Oral Diseases (2012) 18 , 265–270 Background: Increasing evidence indicates that microRNAs (miRNAs) play a vital role in the pathogenesis of inflammatory and autoimmune diseases. The objective of this study was to investigate the altered miRNA expression profile in patients with oral lichen planus (OLP) and determine the miR‐27b expression. Methods: We compared miRNA expression patterns in oral biopsy specimens from patients with OLP (n = 3) with those from normal controls (n = 3) using microarray technology. We further assessed the miR‐27b expression in specimens from patients with OLP (n = 53) against controls (n = 34) using real‐time quantitative PCR (RT‐QPCR), and miR‐27b expression in specimens from patients with OLP (n = 15) against controls (n = 12) using in situ hybridization (ISH). Results: Using microarray analysis, a total of 46 differentially expressed miRNAs with more than 2‐fold change were identified, including 8 up‐regulated and 38 down‐regulated miRNAs. Both RT‐QPCR and ISH analyses revealed that miR‐27b was significantly down‐regulated in OLP tissue, and miR‐27b expression was even more suppressed in atrophic‐erosive OLP than in reticular OLP. In addition, miR‐27b was found to be expressed in the epithelial keratinocyte layer of both normal and OLP tissues. Conclusion: These data indicate that miRNAs may be the novel candidate biomarkers for the implication of miRNAs in the pathogenesis of OLP.     

Gene and microRNA expression reveals sensitivity to paclitaxel in laryngeal cancer cell line

Paclitaxel is a widely used chemotherapy drug for advanced laryngeal cancer patients. However, the fact that there are 20-40% of advanced laryngeal cancer patients do not response to paclitaxel makes it necessary to figure out potential biomarkers for paclitaxel sensitivity prediction. In this work, Hep2, a laryngeal cancer cell line, untreated or treated with lower dose of paclitaxel for 24 h, was applied to DNA microarray chips for gene and miR expression profile analysis. Expression of eight genes altered significantly following paclitaxel treatment, which was further validated by quantitative real-time PCR. Four up-regulated genes were ID2, BMP4, CCL4 and ACTG2, in which ID2 and BMP4 were implicated to be involved in several drugs sensitivity. While the down-regulated four genes, MAPK4, FASN, INSIG1 and SCD, were mainly linked to the endoplasmic reticulum and fatty acid biosynthesis, these two cell processes that are associated with drug sensitivity by increasing evidences. After paclitaxel treatment, expression of 49 miRs was significantly altered. Within these miRs, the most markedly expression-changed were miR-31-star, miR-1264, miR-3150b-5p and miR-210. While the miRs putatively modulated the mRNA expression of the most significantly expression-altered genes were miR-1264, miR-130a, miR-27b, miR-195, miR-1291, miR-214, miR-1277 and miR-1265, which were obtained by miR target prediction and miRNA target correlation. Collectively, our study might provide potential biomarkers for paclitaxel sensitivity prediction and drug resistance targets in laryngeal cancer patients.     

Further results on H ∞ control for discrete‐time uncertain singular systems with interval time‐varying delays in state and input

This paper investigates the state feedback robust H _∞ control problem of a class of discrete‐time singular systems with norm‐bounded uncertainties and interval time‐varying delays in state and input. A new bounded real lemma for discrete‐time singular systems with a pair of time‐varying interval state delays is first investigated. Mathematical comparisons of the new bounded real lemma and two existing ones are presented. Then, on the basis of the bounded real lemma proposed here, a sufficient condition in the form of nonlinear matrix inequality, such that the considered state feedback robust H _∞ control problem is solvable, is given. In order to solve the nonlinear matrix inequality, a cone complementarity linearization algorithm is offered. Several numerical examples are presented to show the applicability of the proposed approach. Copyright © 2012 John Wiley & Sons, Ltd.