草苁蓉提取物在大鼠肝脏化学致癌初期对血清一氧化氮含量和一氧化氮合酶活性的影响

[目的 ]探讨草苁蓉乙醇提取物再经水和二氯甲烷萃取的水层部分在大鼠肝脏化学致癌初期对一氧化氮含量和一氧化氮合酶活性的影响 ,从而进一步探讨其抗致癌作用的可能机理 .[方法 ]按略改良的Solt Farber氏方法制作大鼠肝脏癌前病变模型 ,用二氯化甲烷萃取水层部分 ,饲养大鼠 6周后处死 ,取血液 ,以比色法测定血清一氧化氮含量和一氧化氮合酶活性 .[结果 ]草苁蓉给药组血清一氧化氮含量低于癌前病变模型组 ;草苁蓉给药组血清一氧化氮合酶活性低于癌前病变模型组 .[结论 ]草苁蓉乙醇提取物再经进一步分离的水层成分可降低一氧化氮含量、一氧化氮合酶水平 ,而这可能是其抗致癌作用机理之一     

Chinese Medicinal Herbs in Treating Model Rats with Hepatic Fibrosis

The objective of this study was to examine the effects of Chinese medicine formula-Yu Zhang Dan (YZD, composed of Herba Lysimachiae, Rhizoma Polygoni Cuspidati, Radix Curcumae) on the model rats with hepatic fibrosis. Forty male Sprague-Dawley (SD) rats were used in the present study, and they were separated randomly into 4 groups: a normal control group (Group A, n=5), a model control (Group B, n=15), a high dose of YZD (Group C, n=10), and a low dose of YZD (Group D, n=10). Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl₄). The variation of the serum alanine transaminase (ALT), aspartate aminotransferase (AST), hyaluronate acid (HA), laminin (LN), type • • procollagen peptide (P• •NP), L-Glutathione (GSH) was respectively measured with radioimmunoassay (RIA) and detection of transforming growth factor-beta 1 (TGF-β1) and smooth muscle alpha actin (α-SMA) was conducted with immunohistochemistry. The ALT, AST HA, LN and PIII NP levels in the serum of the model control group were significantly higher than those of the normal control group (P<0.05), and both of the high dose of YZD and low dose of YZD significantly decreased the ALT, AST HA, LN and PIII NP levels of the model rats (P<0.05). The TGF-β1 and α-SMA levels of the model control group were significantly higher than those of the normal control group (P<0.05), and both of the high dose of YZD and low dose of YZD significantly decreased the TGF-β1 levels of the model rats (P<0.05) , and only the high dose of YZD significantly decreased the α-SMA levels of the model rats (P<0.05). The expression of TGF-β1 and α-SMA in the liver tissues of the rats were in the cytoplasm of the cells. It may be through decreasing the ALT, AST, HA, LN and PIII NP levels in the serum of the model rats and decreasing the expression of TGF-β1 and α-SMA in the liver tissues of the model rats that YZD significantly relieved the hepatic fibrosis.     

Study on the Antisenility Effect of Lycium Barbarum L in the Elderly

Ageing can be defined as a time-dependent process whereby one's body can no longer cope with environmental stress and change as easily as it once could. Although each species has a finite and unique maximal life span, there must be ways tonic-taking, for example, to prolong the average life span and prevent premature senility efficiently. The authors investigated on the Lycium barbarum L. grown in Ningxia to see if it can delay, reverse or decrease the rate of decline in normal immune functions and physiological functions. 43 subjects (40 men and 3 women), without overt disease, aged 60 years and over, living in the community and institutions were chosen. Dry fruits of Lycium barbarum L. were orally.     

药用真菌蝉花的研究进展

蝉花是一种传统的药用真菌，除含有与冬虫夏草相似的活性成分外，还具有一些独特的生物活性。现从形态特征、自然分布、人工培养及其所含的活性物质和功能等几个方面评述了药用真菌蝉花的研究现状，且根据当前生物技术的发展趋势，提出了今后研究开发蝉花中一些值得关注的问题，并对其发展前景进行了展望。     

中药资源可持续发展的现状与未来

中药是发展中医药学的物质基础,中医学从古代到现代历经了几千年的时间,中医学不仅吸收了我国传统文化的精髓,也积累了相当丰富的药物应用经验。近年来,国内医学界未能合理开发利用中药资源,只看到社会发展的大量需求,却忽略了中药资源的可持续发展,中药资源被严重消耗,不能实现中药资源再生,最终导致中药资源的可持续发展陷入困境。     

两种治则对实验性骨折成纤维细胞影响的观察

本文介绍运用益气化瘀和益气化瘀补肾两种不同的治则对SD大鼠骨折模型进行治疗,并对骨折断端的成纤维细胞通过电子显微镜作观察。结果表明：益气化瘀法具有增强机体免疫吞噬机能和改善微循环的作用,同时也改善了细胞的营养状况,而益气化瘀补肾法使成纤维细胞的变化对于骨折修复更为有利。因此,中医临床对于骨折治疗采用分期辨证施治,具有其物质基础。     

气相色谱保留指数谱用于中药材鉴别的研究

本文根据规范化的色谱条件测定了６种常用中药－砂仁、当归、苍术、柴胡、藁本和香附的挥发油成分的气相色谱保留指数（ＧＣＲＩ），并从中选择若干代表性成分的ＧＣＲＩ值组成各自的气相色谱保留指数谱（ＧＣＲＩｓ）。实验测得的ＧＣＲＩ值重现性好，３个实验室所测保留指数的ＲＳＤ＜１．０％，研究表明，ＧＣＲＩｓ对于鉴别中药是一种很有前途的手段，特别在中药混乱品种的澄清、指导加工炮制和进行质量评价方面均具有可供实用的前景。本文并利用ＧＣ－ＭＳ和ＧＣ－ＦＴＩＲ对各品种的主要挥发性成分作出了定性鉴别。     

梅国强应用花类中药治疗妇科疾病的经验

目的整理梅国强教授运用花类中药治疗妇科疾病的经验,对临床常用花药按解郁(玫瑰花、绿萼梅、合欢花)、清热(金银花、菊花、野菊花、木槿花)、活血(红花、月季花)、止血(蒲黄、槐花、鸡冠花)、和胃(旋覆花、厚朴花)、其他功用(辛夷花、葛花、扁豆花)共六大类进行简要归纳,供同道参详。     

血栓心脉宁片在血瘀大鼠脑组织中的成分分析

目的 基于血清药物化学方法,研究血栓心脉宁片ig给予病理大鼠后的入脑移行成分。方法 通过冰水浴-肾上腺素法复制急性血瘀大鼠模型,并ig给予血栓心脉宁片[1.4 g/（kg·d）] 8 d,末次ig给药后收集脑组织样本,继而采用超高效液相色谱-四级杆飞行时间质谱联用（UPLC-Q-TOF/MS）技术与多元统计分析相结合的方法快速分析并鉴定吸收入脑的原型成分及其代谢产物。结果 从血瘀大鼠脑组织中鉴定出包括菲醌和蟾酥甾二烯等其他结构类型的11种原型入脑成分及3个代谢产物。结论 UPLC-Q-TOF/MS分析方法的建立全面阐释了血栓心脉宁片的入脑原型成分及其代谢产物,为该中药大品种的药效物质基础研究提供了科学依据。     

关于编制《中国药典》2020年版的几点建议

针对《中国药典》2015年版的现状,遵循建立最严谨的标准的理念,对目前编制《中国药典》2020年版的工作提出关于多基原中药的名称、非标药材进入药典成方、中药基原植物及药材拉丁学名的字体和词序标准化3项建议,为《中国药典》2020年版的修订提供建设性意见。