番茄红素体外抗肿瘤作用实验研究

目的:研究番茄红素体外抑制肿瘤的可能机制。方法 :采用体外细胞培养技术观察番茄红素对人宫颈癌细胞 (Hela)、人肝癌细胞 (QGY- 770 3,Q3)、人肾癌细胞 (786 - O) 3种细胞系细胞凋亡及突变型 p5 3蛋白表达的影响。 结果 :吖啶橙荧光染色后未发现 3种癌细胞 (Hela、Q3、786 - O)有典型凋亡现象 ,Hoechst332 5 8荧光染色后在所试 2种癌细胞 (Hela、786 - O)中无典型凋亡现象。利用 DNA琼脂糖凝胶电泳技术检测细胞凋亡未见 3种 DNA移动呈“梯形”条带。受试物对 Hela、786 - O2种细胞突变型 p5 3蛋白表达的检测与未加受试物的空白对照相比未显示出明显变化。 结论 :番茄红素在体外对所试 3种癌细胞的抑制生长作用可能与促细胞凋亡无关 ,该作用也不是通过抑制突变型 p5 3蛋白表达途径实现的。     

番茄红素对DBP所致果蝇生殖毒性的拮抗作用

目的 研究番茄红素对邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)所致果蝇生殖毒性的拮抗作用.方法 将果蝇随机分为5组,分别给予溶剂对照培养基和含1.0?P及不同浓度番茄红素的培养基喂饲,雌雄配对产子,每日观察并记录子代孵化数和死亡数.结果 与对照组相比,DBP组果蝇的生育率和平均生育数降低,生育期缩短,子代死亡率升高(P＜0.05);随着番茄红素浓度的增加,染毒果蝇的生育率、生育期和平均生育数逐渐提高,子代死亡率逐渐降低.结论 DBP可对果蝇产生生殖毒性,而番茄红素对这种影响有拮抗作用.     

番茄红素联合槲皮素、姜黄素对大鼠慢性前列腺炎/慢性骨盆疼痛综合征的治疗作用以及机制的初步探讨

目的:观察番茄红素联合槲皮素、姜黄素对大鼠慢性前列腺炎/慢性骨盆疼痛综合征(CP/CPPS)的治疗作用,并对其机制进行初步探讨.方法:选取6周龄SD雄性大鼠33只,随机分成正常对照组(n=3)、模型组(n =6)、槲皮素组(n =6)、姜黄素组(n =6)、番茄红素组(n =6)、三素组(n =6),用完全弗氏佐剂诱导...     

Effect of Carotenoids on Paraoxonase-1 Activity and Gene Expression

Paraoxonase 1 (PON1) is an antioxidant enzyme attached to HDL with an anti-atherogenic potential. It protects LDL and HDL from lipid peroxidation. The enzyme is sensitive to various modulating factors, such as genetic polymorphisms as well as pharmacological, dietary (including carotenoids), and lifestyle interventions. Carotenoids are nutritional pigments with antioxidant activity. The aim of this review was to gather evidence on their effect on the modulation of PON1 activity and gene expression. Carotenoids administered as naturally occurring nutritional mixtures may present a synergistic beneficial effect on PON1 status. The effect of carotenoids on the enzyme depends on age, ethnicity, gender, diet, and PON1 genetic variation. Carotenoids, especially astaxanthin, β-carotene, and lycopene, increase PON1 activity. This effect may be explained by their ability to quench singlet oxygen and scavenge free radicals. β-carotene and lycopene were additionally shown to upregulate PON1 gene expression. The putative mechanisms of such regulation involve PON1 CpG-rich region methylation, Ca(2+)/calmodulin-dependent kinase II (CaMKKII) pathway induction, and upregulation via steroid regulatory element-binding protein-2 (SREBP-2). More detailed and extensive research on the mechanisms of PON1 modulation by carotenoids may lead to the development of new targeted therapies for cardiovascular diseases.     

番茄红素调节Keap1/Nrf2/ARE信号通路对精神分裂大鼠认知障碍和氧化应激反应的影响

目的 探究番茄红素调控Kelch样环氧氯丙烷相关蛋白1(Keap1)/核因子E2相关因子2(Nrf2)/抗氧化反应元件（ARE）通路,影响精神分裂症大鼠的氧化应激反应和认知功能。方法 构建精神分裂症大鼠模型,将大鼠随机分为对照组、氯丙嗪组、模型组以及番茄红素高、低剂量组和番茄红素+Nrf2抑制剂组,每组各10例。Morris水迷宫实验检测各组大鼠认知功能;Tunel染色观察并计算各组大鼠海马组织中神经元凋亡率;酶联免疫吸附测定法（ELISA）检测大鼠血清中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）水平;Western blotting法检测各组大鼠海马组织中Keap1、Nrf2和ARE下游抗氧化蛋白HO-1的表达水平。结果 与模型组相比,番茄红素组大鼠水迷宫实验中逃避潜伏期明显缩短（2～5 d）,海马组织细胞凋亡率显著降低,血清CAT、GSH-Px、SOD水平和海马组织Keap1、Nrf2、HO-1蛋白表达量显著升高（P<0.05）;与番茄红素高剂量组相比,番茄红素低剂量组、番茄红素+Nrf2抑制剂组大鼠水迷宫实验中逃避潜伏期明显延长（2～...     

番茄红素对大鼠肺缺血再灌注损伤的保护作用研究

目的：研究番茄红素对大鼠肺缺血再灌注损伤的保护作用,并考察其作用机制。方法取120只SD大鼠随机分为假手术组、模型组、舒血宁注射液（4 mg/kg）组以及番茄红素5、10、20 mg/kg组,每组20只。除假手术组外采用夹闭左肺门45 min后松夹的方法制备大鼠肺缺血再灌注损伤模型。再灌注2 h后,分别测定各组大鼠肺组织湿质量/干质量比值;通过苏木精–尹红（HE）染色观察肺组织病理学改变;原位末端标记（TUNEL）法观察肺组织细胞凋亡并计算凋亡指数（AI）;检测血清中丙二醛（MDA）含量和髓过氧化物酶（MPO）活性;测定肺组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性。结果与模型组比较,番茄红素10、20 mg/kg组肺组织湿质量/干质量比值显著降低（P＜0.05、0.01）,肺组织病变显著改善,肺组织细胞凋亡状况明显改善;血清中 MDA含量和 MPO活性显著降低（P＜0.05、0.01）,肺组织中SOD、CAT活性显著升高（P＜0.05、0.01）。其中番茄红素20 mg/kg组对肺缺血再灌注损伤大鼠肺组织病变改善最为显著、细胞凋亡状况改善以及凋亡指数降低效果最为显著,对血清中MDA含量和MPO活性显著降低效果更加显著、肺组织中GSH-Px活性显著升高（P＜0.01）。结论番茄红素能够有效降低肺组织湿质量/干质量比值,改善肺组织病变,抑制肺组织细胞凋亡、降低凋亡指数,提示番茄红素对大鼠肺缺血再灌注损伤具有剂量相关性的保护作用,其作用机制可能与番茄红素能够有效改善机体抗氧化酶活性、抑制氧化应激损伤有关。     

番茄红素异构体对人卵巢癌HO-8910细胞增殖和凋亡的影响效果初探

目的研究番茄红素异构体对体外培养的人卵巢癌HO-8910细胞增殖和凋亡的影响。方法观察含不同比例的顺、反式异构体番茄红素对人卵巢癌HO-8910细胞的增殖抑制作用;采用PI流式细胞仪检测细胞凋亡率。结果番茄红素异构体对人卵巢癌HO-8910细胞有一定程度的抑制增殖和诱导凋亡的作用,且呈浓度依赖性。结论对人卵巢癌HO-8910细胞,含较高比例顺式番茄红素的Ⅱ号药组抑制增殖与诱导凋亡的作用比全反式番茄红素的I号药组具更强的生物活性。     

番茄红素和大豆异黄酮组合物的毒理学评价

背景与目的:对番茄红素和大豆异黄酮组合物的食用安全性进行毒理学评价.材料与方法:根据<保健食品检验与评价技术规范>进行大鼠经口急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、Ames试验.结果:番茄红素和大豆异黄酮组合物的大鼠经口 LD50>10.0 g/kg;小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验及Ames试验结果均为阴性.结论:番茄红素和大豆异黄酮组合物属无毒级物质,无致突变作用.     

A carotenoid health index based on plasma carotenoids and health outcomes

While there have been many studies on health outcomes that have included measurements of plasma carotenoids, this data has not been reviewed and assembled into a useful form. In this review sixty-two studies of plasma carotenoids and health outcomes, mostly prospective cohort studies or population-based case-control studies, are analyzed together to establish a carotenoid health index. Five cutoff points are established across the percentiles of carotenoid concentrations in populations, from the tenth to ninetieth percentile. The cutoff points (mean ± standard error of the mean) are 1.11 ± 0.08, 1.47 ± 0.08, 1.89 ± 0.08, 2.52 ± 0.13, and 3.07 ± 0.20 μM. For all cause mortality there seems to be a low threshold effect with protection above every cutoff point but the lowest. But for metabolic syndrome and cancer outcomes there tends to be significant positive health outcomes only above the higher cutoff points, perhaps as a triage effect. Based on this data a carotenoid health index is proposed with risk categories as follows: very high risk: <1 μM, high risk: 1-1.5 μM, moderate risk: 1.5-2.5 μM, low risk: 2.5-4 μM, and very low risk: >4 μM. Over 95 percent of the USA population falls into the moderate or high risk category of the carotenoid health index.     

Hepatoprotective activity of aqueous extract of Portulaca oleracea in combination with lycopene in rats

Objective:

To investigate the hepatoprotective activity of the aqueous extract of the aerial parts of Portulaca oleracea (P. oleracea) in combination with lycopene against carbon tetrachloride induced hepatotoxicity in rats.

Materials and Methods:

Hepatotoxicity was induced in male Wistar rats by intraperitoneal injection of carbon tetrachloride (0.1 ml/kg b.w for 14 days). The aqueous extract of P. oleracea in combination with lycopene (50 mg/kg b.w) was administered to the experimental animals at two selected doses for 14 days. The hepatoprotective activity of the combination was evaluated by the liver function marker enzymes in the serum [aspartate transaminases (AST), alanine transaminases (ALT), alkaline phosphatase (Alk.P), total bilirubin (TB), total protein (TP) and total cholesterol (TC)], pentobarbitone induced sleeping time (PST) and histopathological studies of liver.

Results:

Both the treatment groups showed hepatoprotective effect against carbon tetrachloride induced hepatotoxicity by significantly restoring the levels of serum enzymes to normal which was comparable to that of silymarin group. Besides, the results obtained from PST and histopathological results also support the study.

Conclusions:

The oral administration of P. oleracea in combination with lycopene significantly ameliorates CCl₄ hepatotoxicity in rats.