针灸治疗单纯性肥胖症及机理研究探讨

单纯性肥胖症是当今社会常见病、多发病之一。近年来 ,针灸治疗单纯性肥胖症无论在临床方面还是在实验机理研究方面 ,均取得较大进展。但针灸减肥的机理研究大多局限在中枢神经核团神经递质含量的研究 ,没有新的突破 ,在临床方面有待于更多、更科学、更规范的临床研究报道。     

中药炮制机理初探

开展中药炮制机理研究，揭示其炮制内涵变化实质，是确定中药饮片最佳炮制工艺，制定饮片质量标准，提高临床治疗疗效，发展炮制学科理论水平的关键。     

抑胰合剂治疗急性出血坏死性胰腺炎作用机理的实验研究

目的 :探讨抑胰合剂治疗急性出血性坏死性胰腺炎 (AHNP)的作用机理。方法 :36只大鼠随机分成对照组、模型组、治疗组。模型组、治疗组以牛磺胆酸钠胰腺被膜下注射法制作 AHNP模型。治疗组予抑胰合剂 ,均观察 10日。观察大鼠生存率、血清淀粉酶 (AMY)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)的含量变化 ,胰腺组织病理学改变。结果 :治疗组大鼠生存率、血清 AMY、MDA、胰腺组织病理损害程度均低于模型组 ,而 SOD高于模型组。结论 :抑胰合剂可治疗 AHNP,其作用机理可能是通过改善微循环、抑制胰酶活性、清除氧自由基等环节而实现的。     

RT-PCR技术在中药及其活性成分作用机制研究中的应用

RT-PCR是近10年内在中药及其活性部位、活性成分药用研究中应用的分子生物学技术之一，它对于研究中药及其有效成分的作用机制、阐明中药药性理论及其可能的物质基础、建立分子水平上的中药活性检测系统，或以受体和基因为靶点寻找新药具有重要的意义。对RT-PCR技术在中草药复方、单味药及其活性成分的药理活性和临床药用分子机制研究中的应用作一综述，为传统中药的开发和寻找新药提供理论依据和实验基础。     

《金匮》肾气丸义理及衍变

《金匮》肾气丸为温补肾阳之剂，乃阴阳兼顾，阴中求阳，益肾生气，温补肾阳之总方。其在《金匮要略》中治寒湿脚气、虚劳腰痛、痰饮、消渴、转胞凡五病。后世师其法而立新方者甚多，诸如济生肾气丸、十补丸、右归丸、右归饮、六味地黄丸、知柏地黄丸、杞菊地黄丸、麦味地黄丸、左归丸、左归饮等。     

来氟米特的作用机制及临床应用进展

来氟米特是一种新型的免疫抑制剂，主要用于类风湿性关节炎的治疗。近年的一些临床研究提示，该药物对其他自身免疫性疾病也有一定的疗效。本文综述来氟米特的作用机制和临床研究进展，阐述其最新研究动态和发展趋向。     

念珠菌对唑类药物耐药机理的探讨

文献报道念珠菌对唑类药物耐药机制有三，即膜通透性降低、靶酶改变及靶酶产生过多。本文对２６株分离自艾滋病患者的耐氟康唑白念珠菌及通过诱变获得的白念珠菌ＡＴＣＣ１４０５３五个氟康唑耐药突变菌落的耐药机制进行了初步探讨。根据唑类药物靶酶编码序列设计六对上下之间交叉重叠的引物，分六段（包括相当于调控序列所在部位）将目的基因扩增出来，采用Ｓｏｕｔｈｅｒｎ杂交、限制性片段多态性分析及聚合酶链反应－单链构象多态性分析等方法对所获目的片段进行了分析。结果排除了所有受试菌株因靶酶编码基因缺失而耐药的可能，单链构象多态性分析结果呈阳性，且耐药株之间也存在差异。故此，推测耐药性系因为靶酶编码序列多位点突变造成，这一点尚有待进一步证实。另外，本实验结果不能排除另两条耐药机理的存在     

RESISTANT MECHANISMS OF CISPLATIN IN HUMAN LUNG ADENOCARCINOMA CELL LINE A_(549)DDP

ＲＥＳＩＳＴＡＮＴＭＥＣＨＡＮＩＳＭＳＯＦＣＩＳＰＬＡＴＩＮＩＮＨＵＭＡＮＬＵＮＧＡＤＥＮＯＣＡＲＣＩＮＯＭＡＣＥＬＬＬＩＮＥＡ５４９ＤＤＰＺｈａｎＭａｏｃｈｅｎｇ詹茂程ＬｉｕＸｕｙｉ刘叙仪ＣａｉＰｅｎｇ蔡鹏ＸｕＧｕａｎｇｗｅｉ徐光炜ＳｃｈｏｌｏｆＯ...     

Comparison of action of the anti-neoplastic drug lonidamine on drug-sensitive and drug-resistant human breast cancer cells:31 P and 13C nuclear magnetic resonance studies

Lonidamine (LND) is a relatively new anti-cancer drug,and several clinical trials have indicated that it may be effectivein combinations with other therapeutic modalities. LND isclassified within the metabolic inhibitor agents. Multidrugresistance (MDR) phenomenon is often associated with increasedenergy requirements, and enhanced glycolysis rate. These studieswere performed to delineate the mechanism of action of LNDon MDR human breast cancer cells, and to investigate whetherLND as a single agent, or in combination with anotheranti-metabolism drug, 2-deoxyglucose (2-DG), may be usefulagainst MDR tumors. The effects of LND on intact perfuseddrug-sensitive (WT) and 33-fold resistant to Adriamycin(Adr) MCF-7 cells, embedded in alginate micro capsules, were continuouslymonitored by ³¹P and ¹³C nuclearmagnetic resonance (NMR) spectroscopy. ³¹PNMR studies showed that LND induced intracellular acidificationand depletion of NTP in both WT and Adr cells. However, pH and NTPlevels decreased less in the Adr cells than in the WT cells(p < 0.05 for both parameters). ¹³CNMR demonstrated that LND inhibited lactate transport,and lactate signals were elevated in both cell lines. However, theintracellular lactate levels increased to a greater extentin the WT than in the Adr cells (p < 0.05).There were major differences in the effects of LND onmetabolism between sensitive and resistant cells.While LND enhanced glucose uptake in the WT cells, and itsadministration was followed by continuous increase oflactate signal, both processes were not affected by LNDin the Adr cells. 2-DG is a glucose analogue that inhibitsboth cellular uptake and utilization of glucose, leading to cell starvation. Combined treatment with LND and2-DG yielded at best additive, but not synergistic,cellular toxicity, and the metabolic effects of LNDwere attenuated by 2-DG. These results showed that the principalmechanism of action of LND is inhibition of lactate transportleading to intracellular lactate accumulation and acidificationin both WT and Adr cells. The Adr cells were only 2-fold resistantto LND (compared to the WT cells), and since cellular uptakeof alkaloid chemotherapy is improved in acidic environment,LND may have a role in the treatment protocols of MDR tumors,especially when given as the initial means for inductionof intracellular acidification.     

论阿是穴与穴位特异性

中医理论认为,阿是穴是体表具有按之快然或疼痛等特点的位点。从理论上来说,阿是穴包含有具有特异性的经穴和其它常用穴;从功能上来讲,阿是穴实际上与现代医学的牵涉痛的体表部位类似。因此,阿是穴乃是在反映病邪和治疗疾病等两大穴位功能方面具有明显特异性的穴位,本文就此进行了论述。