不同产地藏药余甘子总鞣质含量测定

目的:建立磷钼钨酸/干酪素分光光度法测定不同产地藏药余甘子中总鞣质含量的方法.方法:以没食子酸为对照品,磷钼钨酸为显色剂,检测波长760 nm,测定8个不同产地余甘子中总鞣质含量.结果:没食子酸在0.306～2.448 mg·L-1与吸光度呈良好线性关系(r=0.999 1),平均加样回收率99.27％,RSD 2.30％.不同产地余甘子药材总鞣质含量差别较大(6.72％ ～ 11.95％),印度和尼泊尔产余甘子中总鞣质含量最高.结论:该方法简单、准确、重复性好,可用于测定余甘子中总鞣质含量.     

不同产地叶下珠药材中总酚含量测定

目的:建立不同产地叶下珠药材中总酚的含量测定方法.方法:以柯里拉京为对照品,三氯化铁-铁氰化钾为显色剂,检测波长744 nm,采用UV测定11个不同产地叶下珠药材中总酚的含量.结果:柯里拉京在0.604 ～ 3.02 mg·L-1线性关系良好(r =0.999 1),平均加样回收率99.79％,RSD 2.72％.不同产地叶下珠药材中总酚质量分数5.00％ ～ 12.16％.结论:该方法简单准确、重复性好,可用于叶下珠药材中总酚含量的测定.     

四妙勇安汤及不同提取部位对小鼠急性炎症的影响

目的　观察四妙勇安汤及其不同部位对急性炎症的影响。方法　利用二甲苯致小鼠耳廓肿及腹腔注射 0 .6 %醋酸 (HAc)致小鼠腹腔染料 (Evan’sblue)渗出的方法制作急性炎症模型 ,观察原方及其各部位对急性炎症的影响。结果　原方和氯仿萃取部位对小鼠耳廓肿胀度有明显的抑制作用 (P <0 .0 5 ) ;氯仿萃取部位对小鼠腹腔毛细血管通透性的增加有明显的抑制作用 ,并呈现一定的量效关系 (P <0 .0 5 ,P <0 .0 1)。结论　四妙勇安汤原方及氯仿萃取部位具有显著的抗炎作用。     

Comprehensive evaluation of allele frequency differences of MC1R variants across populations

The melanocortin 1 receptor (MC1R), a member of the G protein-coupled receptors superfamily, mediates the response to melanocortins and is currently the best-described contributor to normal pigment variation in humans. A remarkably large number of natural polymorphisms, or variants, of the MC1R gene have been identified in different populations. Some of these variants have been associated with specific hair and skin color phenotypes, the presence of freckling, and melanoma and nonmelanoma skin cancer risk. Interestingly, some MC1R variants have been associated with skin cancer beyond their effects on pigmentation. Although the red hair color variants (RHC variants) have been associated with skin cancer risk in the Celtic population, studies in darkly-pigmented Caucasian populations have demonstrated the importance of non-RHC MC1R variants on skin cancer risk as well. We have reviewed and compared allele frequency differences of MC1R variants across geographic regions. We observed large differences in the distribution of variants across populations, with a prominent difference between lightly and darkly-pigmented individuals. Moreover, among Caucasian groups, there were seven variants (p.V60L, p.V92M, p.D84E, p.R151C, p.R160W, p.R163Q, and p.D294H) with significantly different allele frequencies. Exploring differences in allele frequencies of MC1R variants across populations with varying pigmentation and differing skin cancer risk may improve our understanding of the complex relationship between MC1R, pigmentation, and carcinogenesis.     

广东省农村初保10年成果区域层次分析

文章分析了广东省２１个地级市的１１５个有农村初级卫生保健任务的县级单位不同区域地理环境因素对广东省“２０００年人人享有卫生保健”规划目标成果各项指标的影响。结果表明：不同区域经济水平差异显著,以小康县为主的水网和平原区域得分较高,以温饱县和宽裕县为主的山区和丘陵区域得分较低。影响得分的主要因素有指标２（县、乡政府年度卫生事业拨款占两级财政支出的比例）、指标５（集资医疗保健覆盖率）、指标７（卫生而所普及率）及指标８（食品卫生合格率）。     

Diaphyseal Chondroblastoma in the Tibia： One Case Report and Literatures Review

Chondroblastoma iS a rare benign cartilaginous neoplasm that ac-counts for approximately 1%Of all bone tumors and characteristicallyarises in the epiphysis of a long bone,particularly the humerus,femurand tibiat[1].     

抗震作业时间地点与救灾人员心理状态的相关性

目的分析开展抗震救灾工作的时间和地点对救灾人员心理的影响,为针对性地开展心理干预提供依据。方法地震应急期,在灾区采用症状自评量表(SCL-90)对救灾人员进行测试,计算阳性项目数、总均分和各因子分,统计分析时间和地点因素对上述指标的影响。结果①按时间分组,10～12d组SCL-90指标显著高于其他两组(P<0.01),13～15d和16～18d两组间各指标差异无统计学意义(P>0.05)。②按救灾地点分组,汶川组总均分、各因子分高于其他组,差异具有统计学意义(P<0.01)。青川组除强迫、敌对、精神病性因子外,其余因子分高于彭州组,差异有统计学意义。③逐步回归分析,救灾地点和救灾时间对总均分的相关系数分别是12.25和-1.16。结论应急救灾人员的心理状态有明显的时间和地域特征,应根据该特征采取针对性的心理防护措施。     

Sun Exposure,Phenotypic Characteristics,and Cutaneous Malignant Melanoma. An Analysis According to Different Clinico-Pathological Variants and Anatomic Locations (Italy)

Objective The aim of the study was to examine the relation between sun exposure and other selected risk factors and the development of different clinico-pathological variants of cutaneous malignant melanoma (CMM) at different body locations. Methods: We analysed data from a case-control study conducted in Italy between 1992 and 1994 on 542 cases of CMM and 538 hospital controls. Clinico-pathological variants included 391 superficial spreading (SSM), 72 nodular (NM) and 39 lentigo maligna (LMM) melanomas.Results: The risk of SSM increased for a high education level (odds ratio, OR = 1.5, 95% confidence interval, CI, 1.0–2.2), sunburn episodes before age 15 (OR = 1.8, 95% CI, 1.1–2.8), light eye colour (OR = 1.6, 95% CI, 1.1–2.4) and solar lentigines (OR = 1.4, 95% CI, 1.0–1.8). The ORs for total number of nevi >30 were 4.4 (95% CI, 2.9–6.7) for SSM, 5.7 (95% CI, 2.6–12.5) for NM and 2.2 (95% CI, 0.5–9.4) for LMM. The risk of CMM located on the trunk seemed to be higher for a high education level (OR = 1.6, 95% CI, 1.0–2.6) and propensity to sunburn (OR = 1.6, 95% CI, 0.8–3.2). The ORs for sunburn episodes, eye and skin colour, and solar lentigines tended to be higher for CMM located on the face/neck and on the upper limbs than the trunk. Total number of nevi was strongly associated with all anatomic locations.Conclusions: Total number of nevi was the major risk factor associated with all melanoma variants and locations. A higher level of education and sunburn episodes in early childhood appeared to be more strongly related to SSM than to other variants. * Address correspondence to: Andrea Altieri, Istituto di Ricerche Farmacologiche “Mario Negri”, Via Eritrea 62, 20157, Milan, Italy. Ph.:  ⁴ Participants: F. Arcangeli, D. Calista, G. Landi (Cesena), A. Di Landro (Bergamo), P. Puiatti (Torino), C. Catricalà, L. Eibenschutz (Roma), G. Lo Scocco, V. Di Lernia (Reggio Emilia), A. Tosti, C.␣Misciali (Bologna), F. Scardigli, M. Cristofolini (Trento), M. Simonacci, M. Sigona (Macerata), G. Pasolini, A. Manganoni, A. Lonati (Brescia), R. Betti (Milano), A. Barba, F. De Agostini, C. Chieregato (Verona), G. Fabbrocini, N. Balato, P. Santoianni (Napoli), M.G. Bertazzoni, P. Danese (Mantova), I. Stanganelli (Ravenna), S. Moretti, P. Carli (Firenze), M. Iannantuono (S. Giovanni Rotondo), P. Taddeucci (Siena), A. Virgili (Ferrara), G. Fenizi (Foggia), E. Rossi, M. Pini, G. Galbiati (Monza), A. Locatelli (Como).