五苓散证病机、病位探析

五苓散方出于《伤寒杂病论》,为治疗水液代谢障碍的代表方剂。五苓散证病机实质为脾失健运,水液运化失常,津液不能正常布化。五苓散证病位在脾。五苓散功效健脾通阳,蠲饮化津,原为脾失健运,水液运化失常,津液不能正常布化而设。     

小天心穴位考析及理论研究

小天心位于手掌根,大小鱼际交接之凹陷中,操作多用揉、掐、捣、掐揉的方法,具有清热利尿、镇惊安神、明目、通经络、发汗解肌的功效,临床用于治疗惊风、夜啼、小便赤涩、黄疸和斜视、视物不明等病症。本文通过查阅整理古今文献,对小天心的定位、操作方法和临床应用进行详细的梳理和分析,同时从传统与现代医学2个方面探究穴位机理,以期更加...     

面部表浅肌肉腱膜系统及面神经的定位

目的 对面部进行区域化界定,对面部表浅肌肉腱膜系统和面神经的分支重新定位.方法 在头部标本上确定标志点和标志线对面部进行分区,在分区内定位表浅肌肉腱膜系统和面神经分支.依A、B及L3线为坐标,对面神经各分支入肌点进行定位和测量.结果 ①一侧面部分为“三部九区”.②表浅肌肉腱膜系统肌性区位于面下一、二、三区及面中三区和面上三区;混合性区分布于面中二、三区以及面下二区和面下三区的交接部位.面神经颞支和颧支神经入肌点分布于面中二区,颊支和下颌缘支的神经入肌点分布于面下二区.③在面中一区和面中二区内,有少量“网格样”吻合支.结论 “三部九区”法定位面部表浅肌肉腱膜系统和面神经较为准确客观.     

应用模糊数学探讨心系病位、病性特征与基础证的相关性

运用模糊数学方法探讨心系病位、病性特征与基础证的相关性.根据五脏系统辨证体系,结合模糊识别中的“择近原则”,利用MATLAB建立心系基础证模糊识别模型,对1741份心系病案资料进行检验,并分析模型中蕴含的隶属关系.心系病位、病性特征与基础证存在模糊映射关系;大部分隶属关系能够较好的反映心系基础证病位、病性特点,个别隶属关系还提示兼夹病位及病性.利用模糊数学方法能较好的模拟心系病位、病性特征与基础证的模糊映射关系,该思路可应用于五脏系统辨证体系中,为病证规范化研究提供参考.     

上颌第二磨牙第四根管口的解剖定位方法研究

目的本文将探讨上颌第二磨牙第四根管口解剖定位,从而提高临床根管治疗效果,最终提高牙病患者生活质量。方法将100颗上颌第二磨牙标本进行预处理后,使用游标卡尺对标本各不同部位距离进行测定,之后使用日本Olympus耳鼻喉内窥镜系统对标本进行观察,并垂直于标本髓室底拍摄各髓室底图,将所拍摄图像存入计算机内进行角度测量。结果100颗上颌第二磨牙标本发生第四根管口几率为39．00％、发生腭侧根双管几率为1．00％;上颌第二磨牙第四根管口位于近中颊侧（MB）根管口舌侧,腭侧根管口中心点到近颊根管口中心点连线（MB—P）近中。结论对患者进行上颌第二磨牙第四根管口明确定位,可提高患者治疗效果,降低患者根管治疗失败率,最终提高患者的治疗满意度,值得临床推广应用。     

SKF 83566 attenuates the effects of ghrelin on performance in the object location memory task

Increasing research implicates ghrelin, a metabolic signaling peptide, in memory processes including acquisition, consolidation, and retention. The present study investigated the effects of ghrelin on spatial memory acquisition by utilizing the object location memory task paradigm. Given the co-expression of ghrelin and dopamine D₁ receptors within hippocampal neurons, we examined a potential interaction between these two systems on memory performance. When injected into the dorsal third ventricle (D3V) of male Sprague-Dawley rats, proximal to hippocampal tissue, ghrelin (500 pmol) increased the amount of time spent with objects in novel locations. This effect was completely reversed by the D₁ antagonist SKF 83566 (100 μg/kg IP), although when administered alone, the antagonist had no effect on task performance (10-100 μg/kg). We also examined the feeding effects of D3V ghrelin and found that the peptide reliably increased food intake (500 pmol) but that this effect was not blocked by SKF 83566 (100 μg/kg). When given alone, SKF 83566 did not alter food intake (10-100 μg/kg). Our findings indicate that, in addition to an orexigenic effect, ghrelin improves acquisition of spatial location memories. Furthermore, D₁ receptor activation is necessary for ghrelin to improve the encoding of spatial memories but does not impact the increase in food intake elicited by the peptide.     

Subcellular proteomics: Determination of specific location and expression levels of lymphatic metastasis associated proteins in hepatocellular carcinoma by subcellular fractionation

Background

Subcellular fractionation and proteomics form an ideal partnership when it comes to specific location and analysis of intracellular organelles and expression levels of multiprotein complexes. Lymphatic metastasis is the major complicated system involving multiple factors. However, to date lymphatic metastatic mechanism is poorly understood.

Aim

To specifically locate expression site by subcellular fractionation, based on expression levels, interpret the involvement of different lymphatic metastasis associated proteins in hepatocellular carcinoma cell lines with different lymphatic metastasis potential.

Method

Mouse hepatocellular cell lines Hca-F and Hca-P are used to evaluate the location and expression levels of some lymphatic metastasis-associated proteins in the cell by using subcellular fractionation kit and Western blot analysis. The proteins under studies were Gelsolin, JNK and Annexin 7.

Results

Gelsolin was sequestered in cytoplasm, membrane and cytoskeleton in F-cells but in P-cells, it was found in cytoplasm and cytoskeleton .JNK was located in nuclear fraction and cytoskeleton in F and P cells, Annexin7 was in cytoplasm with its two isoforms only at this location, cell membrane and cytoskeleton in F and P cells. With the high expression level of Gelsolin, JNK and Annexin 7 in Hca-F cell line than Hca-P cell line.

Conclusion

With subcellular fractionation specific location of Gelsolin, JNK and Annexin 7 at various cell sites during lymphatic metastasis were determined. High expression levels were found in high lymphatic metastasis potential cell lines which indicate their roles according to different expression sites in the disease.     

神经节细胞胶质瘤头皮脑电图特点及手术预后的相关分析

目的 探讨神经节细胞胶质瘤的脑电图特点及手术预后的相关因素.方法 回顾2014年7月至2020年12月昆明三博脑科医院神经外科诊治的35例经手术切除并病理证实神经节细胞胶质瘤患者的病例资料并进行分析总结,定期随访,并通过对患者的术前脑电图、影像学检查、病史等进行统计分析,预测影响癫痫预后的因素.结果 神经节细胞胶质瘤放...