异黄酮活性成分对Aβ_(1-42)诱导的PC12细胞损伤的保护作用

目的探讨异黄酮活性成分对β淀粉样蛋白(Aβ1-42)诱导的PC12细胞损伤的保护作用。方法从大豆中提取纯化异黄酮活性成分,以Aβ1-42诱导PC12细胞损伤为细胞模型,采用细胞形态学方法、MTT法、LDH法观察不同剂量的异黄酮活性成分对PC12细胞损伤的保护作用。结果异黄酮活性成分能有效地改善PC12细胞的形态,提高细胞存活率(P<0.05),保护组LDH活性低于损伤组(P<0.05)。结论大豆异黄酮活性成分对Aβ1-42诱导的PC12细胞损伤具有显著的保护作用。     

大豆异黄酮对心肌梗死大鼠心室重构的影响(英文)

目的：观察大豆异黄酮对心肌梗死大鼠心室重构的影响。方法：雄性SD大鼠,结扎冠状动脉左前降支,造成心肌梗死。实验分为6组：假手术组,心肌梗死模型组,卡托普利组,大豆异黄酮低、中、高剂量组。在结扎冠状动脉后3h开始,分别灌胃给予0.5%羧甲基纤维素钠(CMC-Na),0.5%CMC-Na,卡托普利50mg·kg~(-1)和大豆异黄酮30,90,270 mg·kg~(-1),qd,共35 d。给药容积均为10 mL·kg~(-1)。最后一次给药后24 h,测定心率(HR)、左室舒张末期压(LVEDP)、左室收缩压峰值(Peak)、左室压最大上升速率/下降速率(±dp/dt_(max))和心肌最大缩短速率(V_(max)),测定全心室质量和体重比(TVW/BW),用天狼猩红染色,在图像分析系统下测量梗死范围(IS)、左心室内径(LVD)、室间隔厚度(ST),心肌间质胶原容积系数(ICVF)和血管周围胶原容积系数(PCVF)。结果：卡托普利、大豆异黄酮90 mg·kg~(-1)和270 mg·kg~(-1)组大鼠的梗死范围分别是(31±s4)%,(32±3)%和(31±5)%,均显著小于心肌梗死模型组大鼠[(38.9±2.9)%,P＜0.01]。心肌梗死组大鼠的TVW/BW,LVD,ICVF和PCVF均比假手术组明显增加(P＜0.01),增加的TVW/BW,LVD,ICVF和PCVF用卡托普利和大豆异黄酮90 mg·kg~(-1),270 mg·kg~(-1)治疗后明显减少[P＜0.01,P＜0.05(SI 90 mg·kg~(-1)组的LVD)]。心肌梗死大鼠的ST显著减小(P＜0.01),而给予卡托普利和大豆异黄酮可增加ST(P＜0.01)。结扎LAD后,大鼠的Peak,±dp/dt_(max)和V_(max)明显降低(P＜0.01),而LVEDP明显升高(P＜0.01),降低的Peak,±dp/dt_(max)和V_(max)在给予卡托普利、大豆异黄酮90 mg·kg~(-1)和270 mg·kg~(-1)后明显升高(P＜0.01)；而升高的LVEDP仅在给予卡托普利治疗后降低。结论：大豆异黄酮能够改善心肌梗死大鼠的心功能和心室重构。     

紫外分光光度法测定大豆异黄酮胶囊中大豆异黄酮含量

目的 建立以紫外分光光度法测定大豆异黄酮胶囊中大豆异黄酮含量的方法。方法 采用紫外分光光度法，检测波长为262nm。结果 大豆异黄酮浓度在9．73～19．46μg／mL范围内与吸光度线性关系良好（r=0．9998），平均回收率为99．76％（RSD=O.64％）。结论 该方法准确可靠，操作简便，稳定性好。     

大豆异黄酮抗血小板聚集作用的实验研究

[目的 ]研究大豆异黄酮对血小板聚集性的影响 .[方法 ]普通饲料中添加 2 0 g/kg大豆异黄酮混合物 ,饲喂GK/Jcl糖尿病大鼠 2 0周 ,测定其血小板聚集率 .利用ODS柱层析和高效液相色谱技术分离大豆异黄酮 ,体外观察大豆异黄酮单体对人血小板聚集率的影响 .[结果 ]长期食用大豆异黄酮的GK/Jcl糖尿病大鼠血小板聚集率显著降低 .在人体外血小板聚集实验中 ,大豆异黄酮均抑制腺嘌呤核苷二磷酸钠、胶原蛋白和肾上腺素诱导的血小板聚集 ,大豆异黄酮类型中金雀异黄素的最强 ,其 5 0 %抑制浓度值分别为 0 38,0 30 ,0 2 3mmol/L .[结论 ]大豆异黄酮具有血小板聚集抑制效应 ,在大豆抗血小板聚集中起着重要作用 .     

大豆异黄酮保健食品中苷及苷元的等度HPLC法测定

目的建立等度高效液相色谱法(HPLC)分离测定保健食品中大豆异黄酮三种苷及其苷元的方法.方法采用Inert-silODS-3(4.6 mmx150 mm,5 μm)色谱柱,以1.5%乙酸-甲醇-乙腈(7022.57.5)为流动相等度洗脱,检测波长260 nm,柱温55℃,流速1.2 mL·min-1.样品用70%的乙醇超声提取,经0.45 μm滤膜过滤后进样.结果HPLC过程于30 min左右完成,大豆苷和黄豆苷的分离度为0.9以上,余者分离度均大于1.5.对4种市售保健食品作了分析.结论方法简便、快速、准确,为含大豆异黄酮的保健食品含量测定及质量控制提供了较为理想的方法.     

Soy-based formulas and phyto-oestrogens: a safety profile

Phyto-oestrogens are non-steroidal plant-derived compounds that possess oestrogenic activity and act as selective oestrogen receptor modulators (SERMs). Among the dietary oestrogens, the isoflavone class enjoy a wide-spread distribution in most of the members of the Leguminosae family, including such prominent high-content representatives as soybean. Phyto-oestrogen research has grown rapidly in recent years owing to epidemiological studies suggesting that diets rich in soy may be associated with potential health benefits. There is a paucity of data on endocrine effects of soy phytochemicals during infancy, the most sensitive period of life for the induction of toxicity. The safety of isoflavones in infant formulas has been questioned recently owing to reports of possible hormonal effects. Infants fed soy formula receive high levels of phyto-oestrogens in the form of isoflavones (genistein, daidzein and their glycosides). To date, no adverse effects of short- or long-term use of soy proteins have been observed in humans and exposure to soy-based infant formulas does not appear to lead to different reproductive outcomes than exposure to cow milk formulas. Soy formula seems to be a safe feeding option for most infants. Nevertheless, much closer studies in experimental animals and human populations exposed to phyto-oestrogen-containing products, and particularly soy-based infant formulas, are necessary.     

大豆异黄酮心血管作用的研究进展

异黄酮是一类具有雌激素样作用的生物活性物质。大豆中的异黄酮主要有两种形式：染料木黄酮和黄豆苷原。近年来发现，大豆异黄酮具有较强的扰动脉粥样硬化和舒血管效应，影响细胞增殖和新生血管的形成，对心血管有保护和调节作用。     

联合补充大豆异黄酮和钙预防去势大鼠骨质疏松的实验研究

目的 研究联合补充大豆异黄酮和钙对去卵巢大鼠骨质疏松的预防作用。方法 将50只3月龄SD雌性大鼠摘除卵巢后按体重随机分成5组：假手术对照组、手术对照组、大豆异黄酮组（40mg／kgBW）、钙组（100mg／kgBW）、钙＋大豆异黄酮组（Ca100mg／kgBW＋SI40mg／kgBW）。连续灌胃饲养3个月后测定大鼠血清骨钙素，左侧股骨测定骨密度和骨钙含量、右侧股骨进行骨组织形态学计量分析。结果 Ca＋SI组血清BGP水平和股骨骨密度均高于其他去卵巢各组（P〈0．05），但低于Sham组（P〈0．05）；Ca＋SI组大鼠股骨的骨钙含量与Ca组、Sham组差异均无统计学意义；在去卵巢各组大鼠中手术对照组、大豆异黄酮组和钙组大鼠的骨小梁数目和骨小梁厚度显著减少，骨小梁间距明显增宽，与钙＋大豆异黄酮组相比有显著差异（P〈0．05）。结论 与单独补充大豆异黄酮或钙相比，大豆异黄酮与钙的联合应用能够更有效地预防去卵巢大鼠骨质疏松的发生。     

葛根异黄酮类成分的药理作用研究进展

葛根Pueraria lobata是药食同源中药,具有多种功效。现代药学研究表明异黄酮类是葛根的主要活性成分,已经分离到53个葛根异黄酮化合物,包括葛根素、大豆苷元、大豆苷、葛根素木糖苷、3''-羟基葛根素、染料木素、染料木苷、鹰嘴豆芽素A、芒柄花素、毛蕊异黄酮等,具有解酒护肝、降血糖、调血脂、缓解绝经性骨质疏松、抗肿瘤、心脏保护、抗炎、神经保护、抗氧化等药理作用。对葛根异黄酮类的药理作用进行综述,以期为该类成分的进一步研究和新药研发提供参考。     

Soy Isoflavone Glycitin (4'‐Hydroxy‐6‐Methoxyisoflavone‐7‐D‐Glucoside) Promotes Human Dermal Fibroblast Cell Proliferation and Migration via TGF‐β Signaling

Glycitin is a soy isoflavone that exhibits antioxidant, antiallergic, and anti‐osteoporosis activities. We investigated the effects of glycitin on dermal fibroblast proliferation and migration. Treatment of primary dermal fibroblasts with glycitin increased cell proliferation and migration. In addition, treatment with 20 μM glycitin for 24 h induced the synthesis of collagen type I and type III at both the mRNA and protein levels. Fibronectin was also increased by 20% after treatment. Matrix metalloproteinase‐1 collagenase was decreased in the media after 24‐h incubation with glycitin, and the synthesis of transforming growth factor‐beta (TGF‐β) mRNA increased approximately twofold in cells following glycitin treatment. Phosphorylation of Smad2 and Smad3 increased after 1 h of glycitin treatment, and phosphorylation continued for 24 h. Furthermore, the phosphorylated form of AKT was increased in glycitin‐treated cells after 3 h and remained higher for 24 h. Thus, glycitin treatment produces anti‐aging effects including increased total collagen in the culture media, decreased elastase, and decreased β‐galactosidase. Together, these results indicate that glycitin stimulates TGF‐β secretion, and the subsequent autocrine actions of TGF‐β induce proliferation of fibroblasts, ultimately protecting skin cells from aging and wrinkling. Copyright © 2015 John Wiley & Sons, Ltd.