高浓度葡萄糖对人骨髓内皮细胞增殖功能的影响

为观察不同高浓度葡萄糖对人骨髓内皮细胞增殖能力的影响．应用人肋骨髓内皮细胞在不同浓度葡萄糖（100、500和1000mg／dl）培养液中增殖、纯化后，经免疫组化染色，光镜及电镜鉴定证实，统计每个平皿中内皮细胞集落生长数量。结果相应为36．33±5．50、36．00±4．68和38．33±3．05．高浓度葡萄糖对内皮细胞增殖能力与对照组比较无显著性差异（P＞0．05）。表明糖尿病血管内皮损伤的发病机制与高糖血症无直接关系。     

中医“从肝论治”法治疗便秘型肠易激综合征肝郁气滞证的临床研究

目的:研究"从肝论治"疗法对便秘型肠易激综合征(IBS-C)肝气郁滞证患者的临床疗效。方法:将60例患者随机均分为两组各30例,其中对照组给予乳果糖及枸橼酸莫沙必利片治疗;治疗组30例患者在对照组治疗基础上给予中医"从肝论治"法治疗,两组患者疗程均为4周,疗程结束后随访6个月,比较两组治疗前后及随访后中医证候评分、汉密尔顿焦虑量表(HAMA)评分、疗效及证候复发率的差异。结果:两组患者治疗后及随访后的中医证候评分及HAMA积分均较治疗前显著改善(P0.01),其中治疗组优于对照组(P0.01、P0.05)。治疗组总有效率优于对照组(86.7%vs 70.0%),随访后对照组证候复发率高于治疗组(56.7%vs 26.7%)。结论:"从肝论治"疗法可改善IBS-C肝郁气滞证患者的临床疗效及焦虑情况。     

Symptom‐based latent classes of persons with irritable bowel syndrome

A large amount of interindividual variability exists in symptom experiences of persons with irritable bowel syndrome (IBS). Thus, consideration of multiple symptoms to identify distinct symptom subgroups may be useful in directing personalized health strategies for symptom management. We aimed to identify latent classes (i.e., subgroups) of persons with IBS who share similar patterns of symptoms using symptom‐related variables (six groups of daily diary symptoms, cognitive beliefs about IBS, and IBS quality of life [QOL]); and to examine how subgroups differed in patient characteristics. Data were derived from a baseline assessment of men and women enrolled in two cognitively‐focused intervention trials (N = 332). Using latent class analysis, four latent classes were identified: Class 1 (low symptoms and good QOL, n = 153), Class 2 (low symptoms and moderate QOL, n = 106), Class 3 (high symptoms with diarrhea and poor QOL, n = 38), and Class 4 (high symptoms with low diarrhea and moderate QOL, n = 35). Diarrhea, being female, less formal education, unemployment, and previous history of major depressive disorder were associated with membership in Class 3. Using these distinct symptom profiles, the next step is to explore underlying mechanisms accounting for symptom burden with the goal of designing tailored interventions to reduce that burden.