收费全文 | 190027篇 |
免费 | 13831篇 |
国内免费 | 4430篇 |
耳鼻咽喉 | 1400篇 |
儿科学 | 3165篇 |
妇产科学 | 3134篇 |
基础医学 | 25107篇 |
口腔科学 | 3494篇 |
临床医学 | 13617篇 |
内科学 | 24727篇 |
皮肤病学 | 2401篇 |
神经病学 | 12497篇 |
特种医学 | 6594篇 |
外国民族医学 | 1篇 |
外科学 | 16177篇 |
综合类 | 25205篇 |
现状与发展 | 21篇 |
一般理论 | 8篇 |
预防医学 | 15399篇 |
眼科学 | 2373篇 |
药学 | 26716篇 |
66篇 | |
中国医学 | 12755篇 |
肿瘤学 | 13431篇 |
2024年 | 355篇 |
2023年 | 2353篇 |
2022年 | 5496篇 |
2021年 | 7797篇 |
2020年 | 6419篇 |
2019年 | 6348篇 |
2018年 | 6437篇 |
2017年 | 7049篇 |
2016年 | 7045篇 |
2015年 | 6608篇 |
2014年 | 7581篇 |
2013年 | 11635篇 |
2012年 | 9343篇 |
2011年 | 11255篇 |
2010年 | 7152篇 |
2009年 | 7213篇 |
2008年 | 8790篇 |
2007年 | 9762篇 |
2006年 | 9268篇 |
2005年 | 8588篇 |
2004年 | 7369篇 |
2003年 | 6571篇 |
2002年 | 5215篇 |
2001年 | 4685篇 |
2000年 | 3937篇 |
1999年 | 3362篇 |
1998年 | 2641篇 |
1997年 | 2776篇 |
1996年 | 2553篇 |
1995年 | 2229篇 |
1994年 | 2148篇 |
1993年 | 1828篇 |
1992年 | 1693篇 |
1991年 | 1595篇 |
1990年 | 1369篇 |
1989年 | 1099篇 |
1988年 | 1103篇 |
1987年 | 977篇 |
1986年 | 886篇 |
1985年 | 1331篇 |
1984年 | 1067篇 |
1983年 | 825篇 |
1982年 | 844篇 |
1981年 | 691篇 |
1980年 | 656篇 |
1979年 | 518篇 |
1978年 | 320篇 |
1977年 | 283篇 |
1976年 | 259篇 |
1975年 | 202篇 |
Background
Acute myocardial infarction (AMI) causes irreversible myocardial damage and release of inflammatory mediators, including cytokines, chemokines and miRNAs. We aimed to investigate changes in the levels of cytokines (IL-6, TNF-α and IL-10), miRNAs profiles (miR-146 and miR-155) and distribution of different monocyte subsets (CD14++CD16-, CD14++CD16+, CD14+CD16++) in the acute and post-healing phases of AMI.Methods
In eighteen consecutive AMI patients (mean age 56.78?±?12.4 years, mean left ventricle ejection fraction – LVEF: 41.9?±?9.8%), treated invasively, monocyte subsets frequencies were evaluated (flow cytometry), cytokine concentrations were analyzed (ELISA) as well as plasma miRNAs were isolated twice – on admission and after 19.2?±?5.9 weeks of follow-up. Measurements were also performed among healthy volunteers.Results
AMI patients presented significantly decreased frequencies of classical cells in comparison to healthy controls (median 71.22% [IQR: 64.4–79.04] vs. 84.35% [IQR: 81.2–86.7], p?=?0.001) and higher percent of both intermediate and non-classical cells, yet without statistical significance (median 6.54% [IQR: 5.14–16.64] vs. 5.87% [IQR: 4.48–8.6], p?=?0.37 and median 5.99% [IQR: 3.39–11.5] vs. 5.26% [IQR: 3.62–6.2], p?=?0.42, respectively). In AMI patients both, analyzed plasma miRNA concentrations were higher than in healthy subjects (miR-146: median 5.48 [IQR: 2.4–11.27] vs. 1.84 [IQR: 0.87–2.53], p?=?0.003; miR-155: median 25.35 [IQR: 8.17–43.15] vs. 8.4 [IQR: 0.08–16.9], p?=?0.027, respectively), and returned back to the values found in the control group in follow-up. miR-155/miR-146 ratio correlated with the frequencies of classical monocytes (r=0.6, p?=?0.01) and miR-155 correlated positively with the concentration of inflammatory cytokines ? IL-6 and TNF-α.Conclusions
These results may suggest cooperation of both pro-inflammatory and anti-inflammatory signals in AMI in order to promote appropriate healing of the infarcted myocardium. 相似文献2. The CGA is administered intravenously (IV) and intranasally (IN) at the dose of 10?mg/kg. Further, its concentration in the plasma, cerebrospinal fluid (CSF) and the whole brain is analyzed by HPLC-UV method.
3. The study observes that CGA is rapidly absorbed in plasma with tmax of 1?min similar to IV route after IN administration. The peak plasma concentration and AUC0–24 are higher by 3.5 and 4.0 times respectively in IV administration, compared to IN delivery that represents the significant less systemic exposure of CGA in IN route.
4. However, the concentration of CGA in the brain is 4, 6.5, 5.3, 5.2 and 4.5 times higher at 30, 60, 120, 240 and 360?min, respectively in IN administration compared to IV administration. The exposure of CGA in the brain after IN administration (AUCbrain, IN) was significantly greater (4 times) as compared to the exposure of CGA in the brain (AUCbrain, IV) after IV administration reflecting significant brain uptake of CGA through nasal route. Therefore, IN delivery of CGA can be a promising approach for the treatment of stroke and neurodegenerative disorders. 相似文献