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101.
人胎儿雪旺氏细胞的体外培养及其纯化研究 总被引:3,自引:1,他引:2
本实验用酶消化法从孕14~19周人胎儿周围神经中分离培养雪旺氏细胞,结合差速贴壁和阿糖胞苷处理进行纯化,根据S-100蛋白免疫细胞化学染色和形态学特征,分析不同时期雪旺氏细胞的纯化程度。实验结果显示:培养4d,倒置相差显微镜下观察,雪旺氏细胞呈典型的双极或三极形,端对端或旋涡状排列,S-100蛋白免疫细胞化学染色呈阳性反应,其比例约占98%;在2~3周内,雪旺氏细胞的纯度维持在85%~90%;培养35d,雪旺氏细胞约占80%;单纯采用阿糖胞苷或差速贴壁处理,培养35d,雪旺氏细胞约占70%;原代培养92d,传代11代细胞生长良好。实验结果表明,多种方法联合使用较单一方法所得的纯度高。本方法快速、简便,能为雪旺氏细胞作为移植材料提供足够的来源。 相似文献
102.
Anders Persson Stefan Pauli Christer Halldin Sharon Stone-Elander Lars Farde Irene Sjgren Gran Sedvall 《Human psychopharmacology》1989,4(1):21-31
The 11C-labelled benzodiazepine antagonist Ro 15–1788 (flumazenil) and positron emission tomography (PET) were used to determine quantitative characteristics of benzodiazepine receptor binding in the neocortex of healthy young men. Saturating doses of unlabelled flumazenil administered i.v., before or together with the ligand-reduced 11C-flumazenil accumulation in the neocortex by about 90 per cent. Saturating doses of unlabelled flumazenil had little effect on the accumulation of radioactivity in the benzodiazepine receptor-poor regions such as pons or white matter. By giving graded doses of unlabelled flumazenil together with the tracer, saturation isotherms were obtained allowing the calculation of receptor density (Bmax) and equilibrium dissociation constant (Kd) values on the basis of certain assumptions Bmax values were in the order of 90 pmol/g and Kd values in the order of 10 nM in the neocortex. Scatchard and Hill plots of the radioactivity data indicated that 11C-flumazenil binds to saturable sites of a homogeneous population. The data indicate that intravenous doses of 1 or 2 mg flumazenil result in a benzodiazepine receptor occupancy of about 50 per cent. The method described should be useful for studying regional differences in benzodiazepine receptor characteristics in the living human brain in healthy subjects and neuropsychiatric disorders, and also in relation to treatment with drugs interacting with benzodiazepine receptors. 相似文献
103.
阳冠华 《安徽卫生职业技术学院学报》2006,5(6):77-78
本文介绍了《正常人体学基础》教材的特点,讨论了本学科教学应注意的问题,强调了要充分地使用标本模型、挂图及多媒体教学,讨论了本学科的学习方法. 相似文献
104.
Human prespermatogenesis between the 8th week of pregnancy and six months after birth was studied in testis material of 28 male foetuses from spontaneous abortions and 81 infants who died from sudden infant death. The foetuses and infants were grouped in 10 age groups. A first steep raise in the numbers of germ cells per 20 tubular cross sections from 22.3 in the first group up to 69.5 in group 3 was observed, i.e. up to the end of the 22nd week of pregnancy. Thereafter, a continuous decrease could be observed modulated by a second slighter increase during the first 4 months after birth. The ratio of germ cells and immature Sertoli cells improves from about 1:20 at the beginning to 1:8 in group 3; afterwards it changes in favour of the immature Sertoli cells down to 1:140 at the end of the study. The initial augmentation of germ cells is interpreted as the effect of a first proliferation wave comparable to that of M-prospermatogonia in other species. The decrease of germ cells is due to the stop of germ cell proliferation and simultaneous high proliferative activity of the immature Sertoli cells. 相似文献
105.
CG Teo 《Oral diseases》2002,8(S2):88-90
Oral hairy leukoplakia (OHL) and Kaposi's sarcoma (KS) are commonly encountered in the HIV-infected patient. A unique feature of OHL is non-cytolytic high level of replication of Epstein–Barr virus (EBV) in the glossal epithelium. The expression of viral-encoded anti-apoptotic proteins concomitant to replicative proteins probably underlies this phenomenon. The question of whether OHL arises from activation of EBV latent in the tongue, or from superinfection by endogenous EBV shed via non-glossal sites or by exogenous EBV remains unresolved. Human herpesvirus 8 (HHV8) is now seen as necessary but not sufficient cause of KS. Expression of HHV8-encoded oncogenic proteins in endothelial cells probably explains the aberrant proliferation of these cells in KS lesions. Studies into why KS is so commonly observed at the palate in HIV-infected patients may provide important clues to its pathogenesis. 相似文献
106.
本文综述本校传染病学教研室1955~1995年间所取得的科研成果。内容包括10余种传染病与寄生虫病的临床和基础研究,其中以华支睾吸虫病、恙虫病等有广东特色的传染病以及伤寒、痢疾、病毒性肝炎等影响人民健康最普遍的传染病为重点。反映了建国以来各个时期本教研室对防治这些传染病所作的贡献。所取得的成果,相当一部分通过多年来的验证,已获得广大传染病工作者所认同或列为常规,部分已获得部委级奖励。现在重温这些成果,可能起承前启后的意义。 相似文献
107.
将2例HBV_2患者血清HBsAg纯化,分析了HBV_2的HBsAg氨基酸组成,并与分子量为630198道尔顿HBV_1的HBsAg三种多肽氨基醚组成比较。实验发现,HBV_2与HBV_1的HBsAg的氨基醚组成有较大区别,且区别指数均在13以上。提示HBV_1和HBV_2的HBsAg在一级结构上存在较大差异;HBV_2可能系一新的乙型肝炎病毒,也可能系由于HBV_1S区核苷酸突变所致的变异株。 相似文献
108.
G. GIORDANO T. D'ADDA L. GNETTI E. FROIO C. MERISIO† & M. MELPIGNANO† 《International journal of gynecological cancer》2006,16(4):1601-1607
In this study, we evaluated the presence of human papillomavirus (HPV) DNA in organs of the female upper genital tract, using nine hysterectomy and salpingo-oophorectomy specimens affected by HPV-positive invasive cervical carcinomas, to establish if cervical HPV infection can spread to upper tracts of the female genital system. HPV DNA was evaluated by polymerase chain reaction (PCR) in all cervical carcinomas as well as in all tracts of the genital system. Then, these data were compared with the results obtained from PCR study of five other hysterectomy and salpingo-oophorectomy specimens (control cases). The criteria used for selection of the control cases were informed consent of the patients for research at the time of surgery, absence of neoplasms, absence of any anatomic lesion caused by HPV in cervix, and external genitalia. All selected cases were squamous cervical carcinomas. PCR analysis revealed HPV DNA in all cases of cervical carcinoma. The HPV DNA was detected as weak positivity on PCR analysis in other organs of the genital system. However, the distribution of HPV DNA varied in the various cases and in the different tracts of the same hysterectomy and salpingo-oophorectomy specimen. We believe that the HPV DNA, detected as a weakly positive signal, in the upper genital tract of patients who have a cervical squamous carcinoma could be a reflection of a latent HPV infection, as well as a sign of the existence of micrometastases containing HPV DNA, which cannot be detected by conventional histologic techniques. 相似文献
109.
人心和山羊心脏上、下腔静脉肌袖大体解剖 总被引:2,自引:2,他引:0
目的:观测人和山羊上、下腔静脉肌袖结构特点,为治疗局灶性心房颤动积累解剖学资料。方法:采用大体解剖方法观测人心(20例)和羊心(40例)的上、下腔静脉及其表面的心肌纤维。结果:人心上腔静脉肌袖的长和宽分别为(1.91±0.39)cm、(2.41±0.26)cm,下腔静脉肌袖的长和宽分别为(1.32±0.32)cm,(3.06±0.52)cm;羊心上(前)腔静脉肌袖的长和宽分别为(1.41±1.00)cm、(1.05±0.26)cm,下(后)腔静脉肌袖的长和宽分别为(0.81±0.48)cm,(1.03±0.28)cm。人和羊心脏的上腔静脉肌袖出现率均为100%;下腔静脉肌袖的出现率分别为88.89%和98%。肌纤维多数来源于右心房前后壁,也有来自于左心房或左右心房的。结论:①上腔静脉肌袖是人和羊普遍存在的解剖结构,人的下腔静脉肌袖出现率明显低于羊,可能是上腔静脉肌袖引起局灶性心房颤动多于下腔静脉肌袖的解剖学基础。②上腔静脉肌袖是构成左右房的又一重要通路。 相似文献
110.
C. Ahlström-Emanuelsson M. Andersson C. Persson† C. Schrewelius‡ L. Greiff 《Clinical and experimental allergy》2004,34(5):731-735
BACKGROUND: Rofleponide palmitate is an esterified glucocorticosteroid pro-drug with a promising pre-clinical profile designed to deliver topical airway treatment for allergic rhinitis and asthma in a novel manner. Thus, the rofleponide palmitate pro-drug is designed to provide topical exposure of the mucosa to the inactive lipophilic drug, which would be locally metabolized to the more hydrophilic and readily cleared drug rofleponide. OBJECTIVE: To examine whether rofleponide palmitate affects nasal symptoms and peak inspiratory flow (PIF) in a pollen-season model of allergic rhinitis and to compare any such effects with those of another glucocorticosteroid (i.e., budesonide). METHODS: During the pollen-free season, 40 patients with strictly seasonal allergic rhinitis received topical nasal spray treatment with an aqueous solution of rofleponide palmitate 400 microg and an aqueous solution of budesonide 128 microg once daily for 10 days in a double-blind, placebo-controlled, and crossover study. After 3 days of drug treatment, individualized allergen challenges were given once daily for 7 days while the treatment continued. The washout periods between each of the challenge series were 2 weeks. Nasal symptoms and PIF were recorded in the morning and evening, as well as 10 and 20 min after each allergen challenge. The mean recordings obtained during the last 3 days of the allergen-challenge series, when symptoms were established and when the treatment had lasted for 8-10 days, were used in the analysis. RESULTS: Both active treatments reduced nasal symptoms and improved nasal PIF compared with placebo (P<0.01-0.001). There was no overall difference in efficacy between rofleponide palmitate 400 microg and budesonide 128 microg. CONCLUSIONS: Topical treatment with aqueous solutions of rofleponide palmitate attenuates nasal symptoms and improves nasal PIF in allergic rhinitis. The overall efficacy of 400 microg of rofleponide palmitate is similar to that of 128 microg of budesonide in the pollen-season model used in this study. 相似文献