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排序方式: 共有258条查询结果,搜索用时 171 毫秒
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目的 miRNA- 122启动子序列的预测、克隆及特异性分析.方法 分别从肝癌细胞系Huh-7及HepG2中扩增出预测的miR-122启动子,并将其克隆至含荧光素酶(firefly luciferase,Fluc)报告基因pGL4.17质粒上,用重组质粒转染Huh-7、HepG2及HeLa细胞,分析启动子的特性.结果 成功预测并克隆出miR-122的启动子序列.重组质粒转染细胞后,启动子能够启动Fluc的表达.用含启动子P1的重组载体pGL4.17-P1转染HeLa细胞后,用两种荧光素酶检测体系测得重组载体中荧光素酶的表达水平显著高于对照组(t =0.000 21,P<0.01及t=0.000 38,P<0.01).结论 Huh-7、HepG2细胞中miR-122表达差异与启动子序列缺失无关.而受miR-122启动子调节的报告基因在非肝细胞系(HeLa)中也表达,表明miR-122启动子不具有肝细胞特异性. 相似文献
73.
A sensitive technic using indium111 chloride was devised to investigate the occurrence of pharyngeal aspiration. Twenty normal subjects and 10 patients with depressed consciousness were studied. Forty-five per cent of the normal subjects aspirated during deep sleep. Normal subjects who did not aspirate were noted to sleep poorly. Seventy per cent of the patients with depressed consciousness aspirated. Aspiration of pharyngeal secretions occurs frequently in patients with depressed sensorium and also in normal adults during deep sleep. Bacterial pneumonia may result when aspirated bacteria are not effectively cleared. This may result when clearance mechanisms are impaired or when they are overwhelmed by large volumes of aspirated secretions. 相似文献
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ObjectiveTo investigate the effect of lncRNA XIST on apoptosis induced by hypoxia.MethodsWe analyzed the expression levels of lncRNA XIST and miR-122-5p using RT-qPCR in hypoxia-induced cardiomyocytes. The mechanism by which lncRNA XIST affects myocardial ischemia was investigated using the cell transfection, CCK-8, and dual-luciferase reporter assays, as well as by flowcytometry, western blotting, and RNA immunoprecipitation.ResultsHypoxic H9c2 cells demonstrated a decrease in their migration and invasion abilities and XIST expression and an increase in the extent of their apoptosis and expression of microRNA-122-5p. Overexpression of XIST significantly increased the H9c2 cell viability, enhanced cell migration and invasion, and decreased cell apoptosis in a hypoxic environment. The luciferase activity of XIST-WT in H9c2 cells co-transfected with XIST-WT and microRNA-122-5p mimics had decreased. The results of RNA immunoprecipitation showed that XIST interacted directly with miRNA-122-5p. Overexpression of XIST decreased the level of miRNA-122-5p significantly. mi-122-5p mimics increased H9c2 cell apoptosis and downregulated FOXP2 expression. Overexpression of FOXP2 upregulated the expression of the Bcl-2 protein in H9c2 cells transfected with microRNA-122-5p mimics and inhibited the expression of HIF-alpha, Bax, and the cleaved-caspase 9 protein.ConclusionlncRNA XIST could regulate the miR-122-5p/FOXP2 axis to attenuate hypoxia-induced H9c2 cardiomyocyte injury. 相似文献
75.
The Potential of hsa-mir-106b-5p as Liquid Biomarker in Prostate Cancer Patients in Indonesia
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Christin H BonnuAnggia N RamadhaniRanu B SaputroSalsabila L SesotyosariR DanartoIndwiani AstutiSofia M Haryana 《Asian Pacific journal of cancer prevention》2021,22(3):837-842
Purpose: This study aims to explore the potential of hsa-mir-106b-5p as a new liquid biomarker for prostate cancer sufferers in Indonesia. Methods: Analysis of hsa-mir-106b-5p expression of two tissue samples from BPH patients and two PCa patients used NanoString nCounter Expression Assay then validated by qRT-PCR using 10 patient urine samples for prostate cancer and BPH. Furthermore, analysis of the role of hsa-mir-106b-5p in prostate cancer was carried out bioinformatically. Results: The results of this study indicated that the expression of hsa-mir-106b-5p in prostate cancer tissue was 1.23 times higher than that of BPH and urine of Indonesian patients (1.72 times). Moreover, this miRNA was upregulated in prostate cancer cells compared to normal cells 1.37 times. The hsa-mir-106b-5p appeared to be involved in the development of prostate cancer through the binding of genes involved in endoplasmic reticulum stress pathways and tumor suppressor genes. Conclusion: hsa-mir-106b-5p could modulate prostate cancer by interfering with the endoplasmic reticulum stress repair pathways and decreasing the expression of tumor suppressor genes involved in many biological processes. These updates our understanding of the role of hsa-mir-106b-5p in cancer and its potential as a candidate of a biomarker for clinical diagnosis of prostate cancer. 相似文献
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目的:对微小核糖核酸122(microRNA-122)的定量检测技术进行评估,探讨其临床应用价值。方法:采用miRFLP技术检测企业校准品、参考品和临床样本,评价该技术的分析性能与临床检测价值。结果:该方法定量下限为183 copies/μL,检测标准曲线183~1500000 copies/μL。高浓度平均回收率113.2%,低浓度平均回收率94.2%,比例系统误差为3.7%。人间精密度变异系数4.2%~1.3%;室间精密度变异系数6.4%~2.5%;批间精密度变异系数4.0%~1.5%;批内精密度变异系数4.1%~1.7%。试剂盒放置4℃ 12小时后的检测结果与预期靶值无明显差异。采集化疗后24小时内43例肿瘤化疗患者的血样,并分离血浆进行miRNA-122检测,将检测结果与现行诊断标准进行ROC方法学分析,CutOff值设定为14136copies/μL,与欧盟公布的PSHT HV健康志愿组(欧洲人群)miRNA-122的13356 copies/μL(95%)基本一致。结论:基于miRNA的微小核糖核酸(miRNA-122)定量检测技术具有性能优良、准确度高、稳定性好等优点,有较高的临床应用价值。 相似文献
78.
Rui Wang Xiaohui Liu Jinxia Wu Haohao Liu Wenjun Wang Xinghai Chen Le Yuan Yueqin Wang Xingde Du Ya Ma Michael D. Losiewicz Xiaofeng Zhang Huizhen Zhang 《Environmental toxicology》2020,35(8):822-830
Microcystin‐leucine arginine (MC‐LR) is a cyclic heptapeptide hepatotoxin produced by cyanobacteria. MicroRNA‐122 (miR‐122) is specifically expressed in the liver. This study focuses on the role of miR‐122 in MC‐LR‐induced dysregulation of hepatic iron homeostasis in C57BL/6 mice. The thirty mice were randomly divided into five groups (Control, 12.5 μg/kg·BW MC‐LR, 25 μg/kg·BW MC‐LR, Negative control agomir and 25 μg/kg·BW MC‐LR + miR‐122 agomir). The results show that MC‐LR decreases the expressions of miR‐122, Hamp, and its related regulators, while increasing the content of hepatic iron and the expressions of FPN1 and Tmprss6. Furthermore, miR‐122 agomir pretreatment improves MC‐LR induced dysregulation of hepatic iron homeostasis by arousing the related regulators and reducing the expression of Tmprss6. These results suggest that miR‐122 agomir can prevent the accumulation of hepatic iron induced by MC‐LR, which may be related to the regulation of hepcidin by BMP/SMAD and IL‐6/STAT signaling pathways. 相似文献
79.
本研究旨在建立一种新型人源化Ph染色体阳性急性淋巴细胞白血病(Ph+ ALL)小鼠异种移植模型.4-6周NOD/SCID小鼠经亚致死剂量60Co全身照射后,给予抗小鼠CD122单克隆抗体腹腔注射,在预处理后24 h内经小鼠膝关节骨髓腔注射Ph+ ALL患者骨髓单个核细胞.于移植后8-12周通过流式细胞术检测受鼠骨髓和脾脏中人源细胞的植入水平及其免疫表型,应用实时定量聚合酶链式反应(RQ-PCR)和荧光原位杂交(FISH)检测受鼠骨髓和脾脏中人BCR/ABL1水平,并通过苏木精-伊红染色和抗人CD19,抗人CD34免疫组化染色评价人源ph+ ALL细胞在受鼠各组织器官中的迁移浸润能力.结果表明,在接受Ph+ ALL患者细胞移植的受鼠骨髓和脾脏细胞中,人源Ph+ ALL(huCD45+ CD19+)细胞不仅有不同程度植入,而且植入细胞具有与ph+ ALL患者相似的细胞形态学、免疫表型和细胞遗传学特征.此外,人源Ph+ ALL细胞还广泛迁移浸润到受鼠的脑、肝脏和肾脏等组织器官中.结论:抗CD122抗体预处理的NOD/SCID小鼠联合骨髓腔注射能够支持Ph+ ALL患者骨髓单个核细胞的有效植入,为人类Ph+ ALL白血病启动细胞鉴定及临床新药筛选研究提供一种新型异种移植模型. 相似文献
80.