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目的:建立测定利奈唑胺在中枢神经系统感染患儿脑脊液中浓度的高效液相色谱法(HPLC)。方法:采用Syncronis C18柱(5 μm,4.6 mm×250 mm);流动相为乙腈:水(含0.2%磷酸)=27∶73;流速1.0 mL/min;检测波长254 nm;柱温30 ℃。结果:利奈唑胺在脑脊液浓度为0.2~40 μg/mL范围内浓度和峰面积线性关系良好,标准曲线为As=0.553 9C-0.019 3(r=0.999 9,n=5)。最低定量限、低浓度、中浓度、高浓度的日内和日间变异RSD均<9%,准确度范围为99%~112%,提取回收率在85%~107%。稳定性考察RSD<8%。结论:该方法准确性高、灵敏度好且操作简便,适用于监测利奈唑胺在中枢神经系统感染患儿脑脊液中的浓度,进而为利奈唑胺的个体化用药提供依据。 相似文献
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Visualization of the amygdalo–hippocampal border and its structural variability by 7T and 3T magnetic resonance imaging
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Johanna Derix Shan Yang Falk Lüsebrink Lukas Dominique Josef Fiederer Andreas Schulze‐Bonhage Ad Aertsen Tonio Ball 《Human brain mapping》2014,35(9):4316-4329
The amygdala and the hippocampus are two adjacent structures in the medial temporal lobe that have been broadly investigated in functional and structural neuroimaging due to their central importance in sensory perception, emotion, and memory. Exact demarcation of the amygdalo‐hippocampal border (AHB) is, however, difficult in conventional structural imaging. Recent evidence suggests that, due to this difficulty, functional activation sites with high probability of being located in the hippocampus may erroneously be assigned to the amygdala, and vice versa. In the present study, we investigated the potential of ultra‐high‐field magnetic resonance imaging (MRI) in single sessions for detecting the AHB in humans. We show for the first time the detailed structure of the AHB as it can be visualized in T1‐weighted 7T in vivo images at 0.5‐mm3 isotropic resolution. Compared to data acquired at 3T, 7T images revealed considerably more structural detail in the AHB region. Thus, we observed a striking inter‐hemispheric and interindividual variability of the exact anatomical configuration of the AHB that points to the necessity of individual imaging of the AHB as a prerequisite for accurate anatomical assignment in this region. The findings of the present study demonstrate the usefulness of ultra‐high‐field structural MRI to resolve anatomical ambiguities of the human AHB. Highly accurate morphometric and functional investigations in this region at 7T may allow addressing such hitherto unexplored issues as whether the structural configuration of the AHB is related to functional differences in amygdalo‐hippocampal interaction. Hum Brain Mapp 35:4316–4329, 2014. © 2014 Wiley Periodicals, Inc . 相似文献
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M. Fox B. Misselwitz M. Halama W. Schwizer M. Fried H. Fruehauf 《Neurogastroenterology and motility》2014,26(5):685-695
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Tanja Djurdjevic MD Alexander Loizides MD Wolfgang Löscher MD Hannes Gruber MD Michaela Plaikner MD Siegfried Peer MD 《Muscle & nerve》2014,49(1):35-39
Introduction: Posterior interosseous nerve (PIN) syndrome is a rare compression neuropathy of the PIN in the region of the supinator muscle, most common by the arcade of Frohse. We aimed to specify ultrasonographic findings in patients with PIN syndrome in comparison to healthy volunteers. Methods: Ultrasound images and clinical data of 13 patients with PIN syndrome confirmed by neurological examination and electrophysiological testing were evaluated retrospectively. Anteroposterior nerve diameters measured at the arcade of Frohse were compared with those of 20 healthy volunteers. The echotexture and the presence of a caliber change of the PIN were additionally assessed. Results: Enlargement of the PIN was seen in all patients with PIN syndrome, but not in volunteers (statistically significant difference in mean diameter P < 0.05). Furthermore, edema and caliber change of the PIN were present in all patients. Conclusions: High‐resolution ultrasound allows for differentiation between patients with PIN syndrome and healthy volunteers. Muscle Nerve 49 : 35–39, 2014 相似文献
989.
目的:探讨腹腔镜下鞘状突高位结扎术治疗交通性鞘膜积液的效果。方法:回顾我院2010年8月~2013年12月间腹腔镜下鞘状突高位结扎术治疗交通性鞘膜积液的患儿共25例,平均年龄5岁,左侧8例,右侧17例,术中发现对侧隐性鞘状突未闭2例。结果:手术均获成功,单侧手术时间15~20min,术中无精索血管和输精管损伤,随访无复发、睾丸萎缩、切口瘢痕等。结论:腹腔镜鞘状突高位结扎术治疗交通性鞘膜积液,操作简单、创伤小、并发症少、复发率低,可同时处理对侧隐匿性未闭鞘状突。 相似文献
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