同种异体肾移植者与正常人NK细胞活性及IL-2R的研究

本文对26例同种异体肾移植者的自然杀伤细胞(NK细胞)活性和白细胞介素Ⅱ受体(IL-2R)水平进行了测定,并设28例健康人作对照组。结果:肾移植者与健康对照组相比,NK细胞活性明显降低,差异有极显著性意义(P<0.001);而IL-2R水平则差异无意义(P>0.05)。并就此方法在临床上的应用进行     

Egg-hatching inhibition in mice immunized with recombinant Schistosoma bovis 28 kDa glutathione S-transferase

The capacity of a recombinant glutathione S-transferase from Schistosoma bovis (rSb 28GST) to protect BALB/c mice against homologous and heterologous infections with, respectively, S. bovis or Schistosoma mansoni has been studied. Two injections of the rSb 28GST and an intravenous boost resulted in a marked specific IgG response on the day of experimental challenge with S. bovis or S. mansoni cercariae. Immunization of BALB/c mice led to a reduction in egg maturation and egg viability after infection with S. bovis or S. mansoni. Adult worm recoveries after an S. bovis challenge infection and tissue egg densities (intestine and liver) in S. mansoni challenge infection were also reduced in the immunized groups, but these differences were not statistically significant. No association between in vitro inhibition of GST enzymatic activity induced by immunized mouse sera and worm burden reduction was recorded. The analysis of the immune response, on the day of perfusion, showed the production of immunoglobulin (Ig)G1, IgG2a and IgG2b specific antibodies and the production of interleukin (IL)-4 and IL-5 by spleen cells after rSb 28GST stimulation. These data suggest that rSb 28GST immunization induces a moderate effect upon egg maturation and egg hatching, suggesting the involvement of similar mechanisms of action and common, but not exclusive, targets during S. bovis and S. mansoni infections. As a consequence, immunization with rSb 28GST may prove useful in affecting the pathology and transmission of African schistosomes.     

Expression and cellular localization of interleukin 8 mRNA and protein in the area of xenogenic bone implant

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Comparative Karyotype of Rat and Mouse Using Bidirectional Chromosome Painting

A comparative karyotype of rat (Rattus norvegicus) and mouse (Mus musculus) based on chromosome G-banding morphology, heterologous chromosome painting results and available gene mapping data is proposed. Whole chromosome painting probes from both species were generated by PARM-PCR amplification of flow sorted chromosomes. Bidirectional chromosome painting identifies 36 segments of syntenic homology and allows us to propose a nearly complete comparative karyotype of mouse and rat (except for RNO 13 p and RNO 19 p12-13). Seven segments completely covered the RNO chromosomes 3, 5, 8, 11, 12, 15 and 18. Eight segments completely covered the MMU chromosomes 3, 4, 6, 7, 9, 12, 18 and 19. The RNO chromosomes 5, 8, 18 show complete homology with the MMU chromosomes 4, 9 and 18, respectively. Bidirectional hybridization results clearly assign 16 segments to subchromosomal regions in both species. Interpretation of the results allows subchromosomal assignment of all the remaining segments apart from seven distributed on chromosomes MMU 15, MMU 10 B2-D3 and MMU 17 E3-E5. The proposed comparative karyotype shows overall agreement with available comparative mapping data. The proposed repartition of syntenic homologous segments between the two species provides useful data for gene-mapping studies. In addition, these results will enable the reconstruction of the karyotype of the Cricetidae and Muridae common ancestor and the definition of the precise rearrangements which have occurred in both mouse and rat lineages during evolution. This revised version was published online in August 2006 with corrections to the Cover Date.     

猪-人-鼠嵌合体动物模型的建立及介导人T细胞对猪异种抗原的耐受

目的： 通过共移植人胎胸腺、胎肝组织及猪骨髓细胞于SCID鼠体内,建立猪-人-鼠嵌合体模型,介导人T细胞对猪异种抗原的免疫耐受。方法:实验鼠分成3组：A组为猪骨髓细胞移植组,B组为人胎胸腺、肝脏组织移植组,C组为猪骨髓细胞＋人胎胸腺+肝脏组织移植组;在移植后每3周,用流式细胞仪测定猪和人嵌合体的水平,直至20周;通过混和淋巴细胞反应确定人T细胞的功能及对猪异种抗原的耐受情况。 结果:猪嵌合体在所有实验鼠（包括共移植人组织的实验鼠）中持续时间均超过20周;在移植人胎组织20周时,T细胞的数量为0.7×106/L,在外周血细胞中所占的百分比为0.14%±0.01%;人嵌合体在所有实验鼠（包括共移植猪骨髓细胞的实验鼠）中持续时间亦超过20周。结论: 通过共移植人胎胸腺、胎肝组织及猪造血干细胞于SCID鼠体内,可以建立猪-人-鼠嵌合体模型,并能诱导人T细胞对猪异种抗原的免疫耐受。     

水苏糖对模拟异种心脏移植超急性排斥反应的抑制作用

目的观察水苏糖对猪到人异种心脏移植超急性排斥反应的抑制作用。方法以人血液体外灌注猪游离心脏为基础，模拟猪到人异种心脏移植的超急性排斥反应模型。实验分为A、B两组：即分别用人血液灌注及人血液加水苏糖灌注猪离体心脏。观察灌注后两组心脏的跳动时间；体外灌注1h后，对两组灌注心脏进行免疫组织化学（测定IgG及IgM的沉积）及病理学分析。结果A组灌注心脏平均跳动时间为（9．5±2．5）min；B组灌注心脏平均跳动时间为（46．8±8．1）min，其中有1个心脏在灌注的1h内一直跳动；两组心脏跳动时间比较，差异有统计学意义（P〈0．01）。A组心肌间质呈弥漫性出血、水肿，血管扩张，内皮细胞肿胀、坏死；免疫组织化学检查显示心肌血管内皮组织中有IgG及IgM沉积。B组心肌间质未见出、凝血和坏死，血管内皮细胞未见肿胀；免疫组织化学检测未见IgG及IgM沉积。结论水苏糖对异种心脏移植超急性排斥反应具有抑制作用。     

HRP法对异种神经移植后再生纤维恢复的形态学研究

目的用辣根过氧化酶(HRP)逆行追踪技术探讨异种神经移植后神经纤维的再生.方法将多次冻融处理后的兔胫神经移植于大鼠坐骨神经,术后第2、4、6、8和10周,将HRP注人大鼠坐骨神经吻合部远侧端.结果移植术后第4周起在L4～5脊神经节见到HRP标记细胞,从第6周在腰段脊髓前角内见到标记细胞,其数量随术后存活期延长而增多.术后4周在移植神经内见少量再生神经纤维,6周后再生神经纤维穿过异种移植神经进入大鼠坐骨神经远侧端.结论自移植术后4周起,移植神经内已有再生纤维并部分恢复了轴浆流,证实了用HRP法可反映移植后神经纤维的再生情况.     

猪心的应用解剖研究

目的：对金华猪的心脏进行观测，为转基因猪心的人体移植提供形态学基础。方法：采用60例新鲜猪心（宰杀4h内），其中50例用游标卡尺直接测量心脏诸结构管径和厚度并进行统计；5例经冠状动脉作钡剂造影、X光摄片，结合实体解剖了解冠状动脉的分支、走行；另5例作ABS冠状动脉铸型。结果：金华猪心脏与人类的心脏有诸多相似，但也有如下差别：①前、后室间沟均偏向右侧，故前面观可见左心室大部，在膈面可见右心室大部；②左、右心耳与心房相比，相对较大；左心室壁厚度最大（24mm）；③左、右冠状动脉均发出旋支；④猪心的后腔静脉直径（20mm）小于人心的下腔静脉直径（34mm）；⑤猪心的肺动脉干直径（24mm）小于人心的肺动脉干直径（38．8mm）。结论：金华猪心与人类心脏极为相似，转基因猪心的人体移植具有解剖学基础。     

Olivacine plus coadjuvants in the treatment of murine leukaemia

Administration of olivacine, 1,5-dimethyl-6H-pyrido[4,3-b] carbazole to mice inoculated with 10⁵, 10³ and 10² leukaemic cells (L₁₂₁₀) produced significant survival in all treated groups which was not altered by association with the naturally occurring nucleoside deoxycytidine. Treatment with olivacine plus heterologous serum caused leucocytosis due to neutrophilia, lymphocytosis and monocytosis, as opposed to treatment with olivacine alone, which produced leucopenia due to neutropenia and lymphocytopenia.