Significance of Rosiglitazone Inhibiting TLR4 Expression in Partial Hepatic Ischemia/Reperfusion of Mice

The effect of rosiglitazone as the ligand of peroxisome proliferator-activated receptor γ (PPARγ) inhibiting the TLR4 expression in ischemic lobes in partial hepatic ischemia/reperfusion injury (IRI) in BABL/C mice and the action of rosiglitazone inhibiting the TLR4 receptor-mediated inherent immune response were investigated. The model of the mouse partial hepatic ische- mia/reperfusion injury was established. All the animals were randomly divided into 3 groups: rosiglitazone group, vehicle (dimethylsulphoxide, DMSO) group and sham operation group. The hepatic samples were collected when mice were sacrificed 0, 2, 4 and 6 h after reperfusion following 1 h ischemia to analyze the acute phase of hepatic IRI. The dynamic expression of TlR4 mRNA was de- tected quantitatively by real-time-PCR, and the levels of TNF-α, IL-10 and ALT in portal vein were determined in all groups. After restoration of blood supply, the expression of TLR4 mRNA in ischemic lobes was detected in 0, 2, 4 and 6 h after reperfusion following 1 h ischemia in rosiglitazone group and vehicle group. The most intensive expression of TLR4 mRNA was present at 4 h after reperfusion in ischemic lobes in vehicle group. As compared with vehicle group, the expression of TLR4 mRNA in ischemic lobes in rosiglitazone group was significantly decreased at 4 h after reper- fusion. The level of IL-10 in portal vein was markedly up-regulated in rosiglitazone group as compared with vehicle group. Contrarily, the levels of TNF-α and ALT in portal vein were markedly down-regulated in rosiglitazone group as compared with vehicle group at every time point in mouse partial hepatic IRI model. Rosiglitazone could alleviate the hepatic IRI by inhibiting TLR4 receptor-mediated inherent immune response.     

腹腔镜肝囊肿去顶开窗术46例临床分析

[目的]探讨腹腔镜肝囊肿去顶开窗术的临床应用价值。[方法]回顾总结46例肝囊肿患者的临床资料。其中单发肝囊肿32例,多发肝囊肿14例,均行腹腔镜去顶开窗术。[结果]44例患者成功行腹腔镜肝囊肿去顶开窗术,手术时间30-85 m in,平均45 m in。平均住院4.5 d。术后随访时间1年,复发2例,均为多囊肝患者。中转开腹2例（囊腔与胆管相通1例,血管损伤1例）。[结论]腹腔镜肝囊肿去顶开窗术具有操作简单,创伤小、痛苦轻,患者康复快、住院时间短等优点。     

基于CT图像的肝脏管道变异类型分析

在术前预现肝脏管道结构，分析管道分支的变异情况，能降低活体肝脏移植手术过程的风险。以肝脏管道系统包含的肝静脉、肝动脉与门静脉的主要分支变异类型为理论依据，在实现这三种肝脏管道三维重建的基础上。针对不同的结构特征，使用基于长度和阈值的方法分别设计相应的算法分析其分支变异类型。     

经导管肝动脉化学栓塞术联合无水乙醇注射治疗肝细胞性肝癌疗效分析

目的:对经导管肝动脉化学栓塞术(TACE)联合B超引导下肝脏瘤体内无水乙醇注射术(PEI)治疗肝细胞性肝癌进行疗效分析。方法:将50例肝癌患者随机分为两组,治疗组(24例)予TACE联合PEI治疗,对照组(26例)单用TACE治疗。结果:治疗组6、12、18个月生存率依次为83.33%、54.17%和33.33%,最长生存30个月以上;对照组仅5例生存达12个月以上,生存率为19.23%。经统计学处理有显著性差异(P<0.01)。结论:TACE联合PEI是提高肝细胞性肝癌患者远期生存率较好的治疗方式。     

胚胎胸腺组织移植加肝动脉插管化疗治疗晚期肝癌16例报告

自１９９０年７月至１９９４年７月，我们为１６例晚期肝癌施行了胚胎胸腺组织移植加肝动脉插管化疗术。结果明显减轻临床症状，改善生存质量和生化免疫指标，使癌肿缩小，生存期延长。与对照组比较，对癌肿缩小优于胚胎胸腺组织移植组；对减轻临床症状、改善生化免疫指标、提高生活质量优于肝动脉插管化疗组；对生存期延长优于两个对照组。     

柳茶对CCl4肝损伤的保护作用及机理初探

柳茶能显著防止ＣＣｌ_４中毒小鼠鼠ＳＧＰＴ，ＳＧＯＴ升高；降低肝ＭＤＡ；提高肝、血ＧＳＨ含量和ＧＳＨ－ｐｘ活性。柳茶能诱导肝微粒体蛋白及细胞色素Ｐ－４５０的合成。表明柳茶的保肝作用可能与其清除自由基、抑制脂质过氧化有关。     

Excision of a choledochal cyst and simultaneous hepatic lateral segmentectomy

We treated a 4-year-old girl with a choledochal cyst (CC) with bilateral intrahepatic involvement. A severe stricture between the enormously dilated left intrahepatic bile duct and the dilated common hepatic duct was found; this necessitated prophylactic hepatic lateral segmentectomy together with excision of the CC to avoid possible stone formation in the cystically dilated left intrahepatic duct. The choice of the combined procedures was based upon long-term results of other patients in our experience. This is the first such procedure to be reported.     

丹酚酸A对培养的大鼠肝星状细胞增殖与胶原生成的作用(英文)

目的:研究丹酚酸A对培养的大鼠肝星状细胞增殖与胶原生成的影响。方法:用链酶蛋白酶与胶原酶对肝脏进行原位灌流消化,Nycodenz密度梯度离心分离大鼠肝星状细胞,传一代培养。MTT法与[~3H]TdR掺入法测定细胞增殖。丽春红染色、图象分析法半定量细胞胶原沉积量,ELISA法测定细胞培养上清中Ⅰ型胶原分泌量,检测细胞层总蛋白量校正细胞数。RT-PCR法分析前胶原α_2(Ⅰ)基因的表达。结果:丹酚酸A 100μmol/L引起部分细胞脱壁与死亡,有一定毒性反应。丹酚酸A 0.1-10μmol/L对细胞形态无明显影响。丹酚酸A1-100μmol/L浓度依赖性抑制细胞增殖,降低胶原沉积量与Ⅰ型胶原分泌量。丹酚酸A1-10μmol/L对前胶原α_2(Ⅰ)mRNA表达均有明显抑制作用。结论:丹酚酸A抑制肝星状细胞增殖与胶原表达,是丹参抗肝纤维化的主要有效成分之一,抑制肝星状细胞活化是其抗肝纤维化的主要作用机制。     

Hepatic Disposition of Fexofenadine: Influence of the Transport Inhibitors Erythromycin and Dibromosulphothalein

Purpose. To examine the disposition of fexofenadine in the isolated perfused rat liver and the influence of erythromycin and dibromosulphthalein (DBSP) on the hepatic uptake and biliary excretion of fexofenadine. Methods. Livers from four groups of rats were perfused in a recirculatory manner with fexofenadine HCl added as a bolus (125, 250, 500, or 1000 g) to perfusate. Livers from another three groups of rats were perfused with 250 g of fexofenadine HCl. With one group as control, erythromycin (4.0 g/ml) or DBSP (136 g/ml) was added to the perfusate of the other groups. In all experiments, perfusate and bile were collected for 60 min; in addition, livers from the second experiment were retained for assay. Fexofenadine was determined in perfusate, bile, and homogenized liver by HPLC. Results. The area under the curve (AUC) of fexofenadine was linearly related to concentration. It was unchanged from control (12,800 ± 200 ng·h/ml) by erythromycin (14,400 ± 2000 ng·h/ml), but was increased 95% by DBSP (25,000 ± 2600 ng·h/ml, P <0.001). The ratios of the concentrations of fexofenadine in liver/perfusate were decreased significantly by DBSP; those for bile/liver were increased by erythromycin. Conclusions. Erythromycin reduced the canalicular transport of fexofenadine into bile, whereas DBSP reduced uptake across the sinusoidal membrane.     

肝静脉的应用解剖学研究

目的　为活体近亲肝移植和肝左叶切除术提供解剖学基础。方法　在 4 0例成人尸体肝膈面切除肝实质 ,显露肝静脉 ,观测肝静脉末端直径和距离肝膈面的深度。结果　肝左静脉长度 3.0 3± 1.1cm ;末端直径 1.13± 0 .2 6 cm ;距离肝静脉在下腔静脉汇入口 1～ 3cm的深度分别为 1.15± 0 .4 9cm ,1.6± 0 .5 9cm ,1.91± 0 .6 4 cm;与镰状韧带间的夹角为 30 .2 9±12 .33°。肝中静脉长度 5 .2 8± 1.14 cm ;末端直径 1.0 5± 0 .2 5 cm ;距离肝静脉在下腔静脉汇入口 1～ 4 cm的深度分别为 1.78± 0 .5 1cm,2 .78± 0 .6 8cm ,3.71± 0 .6 9cm,4 .16± 0 .71cm ;与镰状韧带之间的夹角为 4 5 .0 8± 10 .15°。肝右静脉长度 4 .6 7±1.2 9cm ;末端直径 1.33± 0 .2 7cm ;距离肝静脉在下腔静脉汇入口 1～ 4 cm的深度分别为 1.0 2± 0 .5 4 cm ,2 .0± 0 .74 cm ,2 .81± 0 .81cm,3.6 5± 0 .71cm;肝左、中、右静脉末端游离部长度分别为 0 .5 5± 0 .2 9cm ,0 .4 5± 0 .16 cm和 0 .5 4± 0 .2 5 cm。结论　肝静脉主干在肝实质内的行程是斜行的 ,其起始部距离肝膈面较深 ,终末部较浅 ,肝左、右静脉末端 1cm均距肝膈面 1cm深 ,手术中容易显露 ,但容易损伤。肝左、中静脉在膈面与肝镰状韧带间的夹角分别呈 30°和 4