Evaluation of wound healing properties of Arrabidaea chica Verlot extract

ETHNOPHARMACOLOGICAL RELEVANCE: Arrabidaea chica Verlot. (Bignoniaceae), popularly known as Crajiru, has been traditionally used as wound healing agent. AIM OF THE STUDY: Investigate in vitro and in vivo healing properties of Arrabidaea chica leaves extract (AC). MATERIALS AND METHODS: AC was evaluated in vitro in fibroblast growth stimulation (0.25-250 microg/mL) and collagen production stimulation (250 microg/mL) assays. Allantoin (0.25-250 microg/mL) and vitamin C (25 microg/mL) were used as controls respectively. DPPH and Folin-Ciocalteau assays were used for antioxidant evaluation, using trolox (0.25-250 microg/mL) as reference antioxidant. To study wound healing properties in rats, AC (100mg/mL, 200 microL/wound/day) was topically administered during 10 days and wound area was evaluated every day. Allantoin (100mg/mL, 200 microL/wound/day) was used as standard drug. After treatment, wound sites were removed for histopathological analysis and total collagen determination. RESULTS: AC stimulated fibroblast growth in a concentration dependent way (EC50=30 microg/mL), increased in vitro collagen production and demonstrated moderate antioxidant capacity. In vivo, AC reduced wound size in 96%, whereas saline group showed only 36% wound healing. CONCLUSION: AC efficiency seems to involve fibroblast growing stimulus and collagen synthesis both in vitro and in vivo, beyond moderate scavenging activity, corroborating Crajiru folk use.     

Wound healing, anti-microbial and antioxidant potential of Dendrophthoe falcata (L.f) Ettingsh

Dendrophthoe falcata (L.f) Ettingsh (Loranthaceae) of the order Santalales, is used ethnomedicinally for treating ulcers, asthma, impotence, paralysis, skin diseases, and wounds. In this context, validations of the ethnotherapeutic claims of the plant in wound healing activity was studied, besides anti-microbial activity and antioxidant activity were performed to understand the mechanism of wound healing potency. The ethanolic extract of aerial parts of Dendrophthoe falcata ethanolic extract (DFEE) was investigated for the evaluation of its healing efficiency on excision and incision wound models in rats. The results showed that Dendrophthoe falcata extract has potent wound healing capacity as evident from the wound contraction and increased tensile strength. Hydroxyproline and hexosamine expressions were also well correlative with the healing pattern observed. Three of the fractions A-C (petroleum ether, chloroform and ethanol, respectively) obtained from the extract exhibited significant antimicrobial activity against the organisms: Staphylococcus aureus, Staphylococcus pyogenes, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Enterobacter aerogenes, Pseudomonas aeroginosa, Serratia marcescens, and five fungi Candida albicans, Candida tropicalis: dimorphic fungi, Aspergillus fumigatus, Aspergillus niger: systemic fungi, and some infectious bacteria Escherichia coli, and Salmonella typhi. The results also indicated that DFEE possesses potent antioxidant activity by inhibiting lipid peroxidation, reduced glutathione, superoxide dismutase levels and increased the catalase activity.     

Effect of honey on intestinal morphology, intraabdominal adhesions and anastomotic healing

In the present study, attempts have been made to determine the effects of honey on intestinal morphology, postoperative adhesions, and the healing of colonic anastomoses in the rats after colonic resection and anastomosis. Thirty-six rats were randomly divided into three groups each including 12 animals. Colonic resection and anastomosis were performed on all animals. Rats were fed with standard rat chow in group I, standard rat chow plus 10 g/kg/day honey in group II and artificial honey including the same caloric amount with honey in group III. Adhesion scores, bursting pressures and histopathological examinations were evaluated. Colonic bursting pressures of honey group were significantly better than control and artificial honey groups. Histological analysis of anastomotic site showed that submucosa and muscularis propria were nearly filled with granulation tissue and regular fibrin matrix in honey group. There was statistically significant difference between the adhesion scores of honey vs artificial honey and control groups. The scores of histological changes of ileum in honey group were significantly different from other groups. These results indicate a protective role of honey against intraabdominal adhesions and anastomotic dehiscence.     

Evaluating healing for cancer in a community setting from the perspective of clients and healers: a pilot study

The real-life practice of ‘healing’ for cancer in the community as perceived by clients and healers was investigated in a multi-method pilot study. Fifteen clients received six weekly healing sessions. Pre- and post-changes in perception towards well-being and client experience were assessed by EuroQol (EQ-5D), measure yourself concerns and well-being (MYCaW) and a client satisfaction tool. Qualitative methods, including focus groups, explored the perceived effects of healing in more depth and the participants’ experience of taking part in research. The study was not designed to test the effect of healing on disease.

Quantitative data showed perceived significant improvements in ‘concerns/problems’ for which clients wanted help (p<0.01), well-being (p<0.01) and anxiety/depression (p<0.05) over the course of healing. Significant effects were not seen in all areas of quality of life. Qualitative analysis showed clients mainly sought help for psychological and emotional concerns and reported only beneficial effects of healing. Clients attributed many of the quantitative improvements to healing itself. Despite some concerns, healers and clients engaged fully with the research process, and were enthusiastic about the importance of research into healing.

Our study suggests that, while there are some confounding issues and study limitations to address, clients and healers perceive healing to have a range of benefits, particularly in terms of coping with cancer, and regard it as a useful approach that can be applied in a community setting alongside conventional medicine.     

手足部深度烧伤患者早期整形修复的临床疗效分析

目的探讨手足部深度烧伤患者早期整形修复的临床疗效。方法总结治疗的手足部深度烧伤患者56例资料,按照治疗时间进行分组：早期整形恢复治疗（7d内治疗）的治疗组30例,7d后治疗的对照组26例,治疗后按照文中疗效标准统计各组疗效和创面愈合情况,统计学比较组间差异。结果治疗组创面愈合平均时间为（28．9±75）d,低于对照组平均数据（P〈0．05）,治疗组疗效结果优秀者18例,疗效良好者8例,总优良率86．7％,对照组疗效结果优秀者8例,疗效良好者7例,总优良率57.7％,统计学结果表明组间疗效具有显著性差异（P〈0.05）。结论早期整形修复方法治疗手足部深度烧伤患者具有满意临床疗效。     

MEBT/MEBO对皮肤创面愈合过程中VEGF、bFGF、EGF mRNA表达影响的研究

目的探讨MEBT/MEBO促进创面愈合的作用机制。方法建立大鼠急性损伤创面模型,观察MEBT/MEBO对模型创面组织VEGF、bFGF、EGF mRNA表达的影响。结果①创面肉芽组织生长大体情况:造模后第8天,MEBT/MEBO组、贝复济组大鼠创面肉芽组织生长情况明显好于模型组,MEBT/MEBO组和贝复济组大鼠创面较红润,可见散在的肉芽颗粒生长,模型组大鼠创面较苍白,生长缓慢。②愈合时间:MEBT/MEBO组大鼠创面平均愈合时间为(12.50±0.83)天,短于贝复济组(13.15±0.88)天,明显短于模型组(14.20±1.28)天,MEBT/MEBO组与贝复济组、模型组在愈合时间方面相比差异均有显著性(P<0.05,P<0.01)。③VEGF、bFGF、EGF mRNA表达:模型组大鼠的表达量在造模后8天时相点显著低于MEBT/MEBO组、贝复济组(P<0.01),MEBT/MEBO组在造模后8天时相点比贝复济组的表达量稍高,差异有显著性(P<0.05)。结论①MEBT/MEBO可以提高大鼠肉芽组织中VEGF、bFGF、EGFmRNA的表达水平,推测MEBO可能通过对VEGF、bFGF、EGF的调控,促进创面成纤维细胞的分裂增殖及新生毛细血管的增殖,从而促进肉芽组织形成,加速创面愈合。②MEBT/MEBO能明显缩短实验性SD雄性大鼠体表创伤创面修复愈合时间,具有促进大鼠体表创伤创面愈合的作用。     

重组人生长激素对烧伤创面愈合的影响

目的 评价重组人生长激素对烧伤创面愈合的影响。方法 ８４例不同烧伤病人分成给药组和不给药组。给药组予以皮下注射ｒｈＧＨ,其他治疗与不给药组相同,分别观察创面愈合时间,住院天数,测定严重烧伤病人２４ｈ尿尿素氮（ＵＵＮ）排出量。结果 应用ｒｈＧＨ的病例创面愈合时间明显缩短,住院天数明显减少,严重烧伤病人２４ｈＵＵＮ排出量明显减少。结论 ｒｈＧＨ能促进烧伤病人蛋白质合成,加速创面愈合。     

毛囊干细胞合成β-NGF及其受体p75NTR对皮肤创伤的早期修复作用

目的以切除全层表皮为激发条件,排除表皮干细胞对创伤部位进行愈合修复的影响,同时检测在愈合修复过程中β-NGF及其受体p75NTR的表达,探讨它们对创伤愈合以及HFSC分化和迁移的可能影响。方法随机选择36只乳鼠,利用外科微创手术制作在体创伤模型,使用超薄冰冻切片和免疫组织化学技术,检测创伤修复过程中2～12 h时间点的β-NGF及其受体p75NTR对早期创伤修复作用,使用毛囊干细胞特异性标志物描述其变化。双盲半定量方法检测阳性表达强度。结果组内分析结果显示全层表皮创伤后β-NGF的变化无具有统计学意义;p75NTR的变化在2、4和6 h与其他时间点差异具有统计学意义（P〈0.05）。组间分析结果显示全层表皮创伤后β-NGF与对照组相比在2～12 h差异统计学意义（P〉0.05）;p75NTR与对照组相比在4 h差异具有统计学意义（P〈0.05）。干细胞标志物β1-integrin,CD34和K15无明显变化,但是在12h时间点有下降趋势。结论β-NGF与p75NTR的表达不一致性体现在4h,提示p75NTR可能参与创伤发生时规律性的变化。毛囊干细胞SC特异性标志物β1-integrin、CD34和K15的降低提示毛囊干细胞可能在这一时期开始发生转变。     

封闭式负压引流治疗严重软组织缺损的疗效分析

目的 探讨封闭式负压引流技术（vacuum sealing drainge,VSD）处理创伤性大面积皮肤软组织缺损创面的效果.方法 对120例创伤性大面积皮肤软组织缺损的创面处理分别采用VSD技术（VSD组,60例）和常规换药治疗（对照组,60例）,比较两者疗效.结果 VSD组创面清洁时间为6～12（7.6±1.5）d;45例植皮,15例皮瓣修复.对照组创面清洁时间为12～20（15.6±4.3）d：32例植皮,28例皮瓣修复.两组在创面清洁时间、住院时间及植皮效果方面差异均有统计学意义（P〈0.01）.结论 VSD技术对创伤性大面积皮肤软组织缺损创面的处理效果优于传统换药.     

糖尿病创面愈合与GM-CSF关系的研究

目的探讨糖尿病创面愈合与粒细胞巨噬细胞集落刺激因子（GM-CSF）的关系。方法70只C 57B L/6小鼠分为野生小鼠组（对照组,n=35）和糖尿病模型组（DM组,n=35）。腹腔麻醉后在背部中线两侧各制作0.8cm×0.8cm创面。创面动态摄像并于相应时间段取标本,观察创面组织愈合情况,同时计算创面愈合率;EL ISA法测定创面GM-CSF表达。结果创面形成后第3天起,DM组小鼠创面愈合率较对照组明显下降,以创面形成后7 d内变化最为明显;创面形成后第1天,两组小鼠创面GM-CSF表达均明显增高;创面形成后第1天和第3天,对照组小鼠创面GM-CSF表达显著高于DM组。结论GM-CSF的低表达可能与创面愈合早期炎症细胞浸润减少有关。