Synergistic enhancement of collagenous protein synthesis by human gingival fibroblasts exposed to nifedipine and interleukin-1-beta in vitro

Abstract: Gingival overgrowth commonly occurs coincident to therapy with calcium channel blockers. The biologic mechanism for this condition is unknown; however, many clinicians suggest that poor oral hygiene may contribute to development of the overgrowth. This study tests the hypothesis that collagenous protein synthesis by gingival fibroblasts is synergistically enhanced when they are exposed to both nifedipine (N) and the pro-inflammatory cytokine, interleukin-1-beta, a cytokine expressed in inflamed gingiva. Human gingival fibroblasts were isolated from biopsies of normal gingiva and cells separated into two groups. Group 1 was exposed to media containing 0, 5, 50, or 500 pg/ml IL-1-beta, or 10⁻⁷ M N for 7 days; Group 2 was exposed to those concentrations of IL-1-beta +10⁻⁷ M N. [³H]-proline was added to the medium for the final 24 h. Cells and matrix were harvested and radioactivity determined by liquid scintillation analysis. Means (d.p.m./10³ cells) were compared by factorial ANOVA and Scheffè comparisons. Collagenous protein synthesis was significantly reduced by 5 pg/ml IL-1-beta +10⁻⁷ M N and enhanced by 500 pg/ml IL-1-beta +10⁻⁷ M N as compared to N or IL-1-beta alone. Thus, patients may be more susceptible to gingival overgrowth coincident to nifedipine therapy as a result of the synergistic enhancement of connective tissue synthesis by these agents.     

健儿止泻颗粒联合丁桂儿脐贴对小儿腹泻疗效及血清炎症因子的影响

目的:观察健儿止泻颗粒联合丁桂儿脐贴对小儿腹泻患者临床疗效及血清炎症因子的影响。方法:选取小儿腹泻患者148例,随机将其分为观察组及对照组,每组74例,对照组患者予西医常规抗感染及对症治疗,观察组患者在西医治疗基础上予健儿止泻颗粒联合丁桂儿脐贴治疗,比较两组患者临床疗效、不良反应以及对血清炎症影响因子的影响。结果:观察组临床疗效与对照组比较有显著性差异,明显优于对照组(P<0.05),观察组患者不良反应明显低于对照组,两组比较有显著性差异(P<0.05)。经治疗后观察组CRP、IL-6、IL-10、TNF-α水平均明显低于对照组,且两组之间比较均有显著性差异(P<0.05)。结论:健儿止泻颗粒联合丁桂儿脐贴对小儿腹泻患者临床疗效显著,值得在临床上进一步推广。     

Attenuation of 5-HT1A and 5-HT2 but not 5-HT1C receptor mediated behaviour in rats following chronic treatment with 5-HT receptor agonists,antagonists or antidepressants

The effects of chronic (10 days) treatment with serotonin (5-HT) receptor agonists on 5-HT_1A receptor mediated lower lip retraction (LLR), 5-HT_1C receptor mediated penile erections (PE) or 5-HT₂ receptor mediated head shakes (HS) were studied in rats. It was found that the 5-HT_1A and 5-HT₂ receptor mediated behaviour could be attenuated after chronic treatment, whereas 5-HT_1C receptor mediated behaviour remained unchanged. The ED₅₀ for the 5-HT_1A receptor mediated, 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT)-induced LLR showed an increase from 0.07 mg/kg in placebo pretreated rats to 0.13 in 8-OH-DPAT (1mg/kg/day) pretreated rats. The number of 5-HT₂ receptor mediated (±)-1-2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) (0.46 and 1 mg/kg)-induced HS was significantly reduced (67% and 50%, respectively) after 10 days' pretreatment with DOI (1 mg/kg/day). In the same animals the number of 5-HT_1C receptor mediated PE was increased. Ten days' pretreatment with MK 212 (0.46 mg/kg/day) failed to affect MK 212 (0.22 and 0.46 mg/kg)-induced PE. In addition, the effects of chronic treatment with some antidepressants were studied. The monoamine oxidase (MAO) inhibitor tranylcypromine (4 mg/kg/day) given for 10 days caused an increase in the ED₅₀ for 8-OH-DPAT induced LLR (ED₅₀ values were 0.06 and 0.14 mg/kg, respectively, in placebo — and tranylcypromine — pretreated rats) and attenuated MK 212 (0.22 and 0.46 mg/kg)-induced PE. Chronic treatment with mianserin (10 mg/kg/day), a tetracyclic antidepressant with 5-HT_1C and 5-HT₂ receptor antagonistic properties, did not change PE induced by MK 212, but caused an increase of PE induced by DOI and a decrease of DOI-induced HS. Ten days' pretreatment with the 5-HT re-uptake inhibitor Org 6997 (5 mg/kg/day) had no effect on MK 212-induced PE. The results demonstrate that 5-HT_1A and 5-HT₂ but not 5-HT_1C receptor mediated behaviour can be attenuated by chronic treatment with agonists for these receptors. The 5-HT_1C receptor mediated behaviour remains unchanged in response to chronic agonist treatment. Chronic treatment with antidepressants have differential effect on these behaviours. The possible implication for the mechanism of action of antidepressants is discussed.     

Vitapex充填根管后的临床观察

目的：比较Vitapex糊剂单独使用及与牙胶尖联合使用充填根管后的临床疗效。方法：A组单独使用Vitapex粗剂，B组使用Vitapex胡剂加牙胶尖，C组使用传统氧化锌丁香油糊剂加牙胶尖进行根充，与治疗前及随访1年后拍摄根尖周片对比。结果：三组成功率分别为96.70%,98.90%,89.01%。其中A，B组无显著差异（P＞0．05），A，C组与B，C组差异均显著（P＜0．05）。结论：Vitapex糊剂是一种新型，简便的根充材料，单独或与牙胶尖联合使用均能起到较好的疗效，值得临床推广应用。     

High-power diode laser on management of drug-induced gingival overgrowth: Report of two cases and long-term follow-up

Background: Drug-induced gingival overgrowth (DIGO) is attributed mainly to the prolonged use of calcium channel blocking agents, anticonvulsants, and anti-calcineurin inhibitors . The management of DIGO depends on the severity of the disease and includes surgical intervention and plaque control. Compared to conventional surgical treatment, the recent literature data have shown that gingivectomy using a high-power laser (HPL) is a short and easy procedure, which results in minimal postoperative discomfort and greater patient accep- tance. Objective and Methods: The purpose of this study was to report two cases of recurrent DIGO treated surgically using HPL (λ 808nm, 1.5W). Results: Minimal bleeding and discomfort were observed during surgery, and patients reported no pain or bleeding after the procedure. After 1 year of follow-up, patients presented a minimal increase of gingival volume, indicating that laser technology can improve the efficiency and prognosis of DIGO. Conclusions:The HPL was able to preventing hemorrhage and improving the patient’s collaboration with the treatment and quality of life.     

复方黄倍软膏抗过敏及止痒作用的实验研究

目的观察复方黄倍软膏的抗过敏及止痒作用。方法通过二硝基氯苯丙酮溶液诱发小鼠迟发变态反应和磷酸组胺诱发豚鼠局部瘙痒的实验模型,以不同浓度的复方黄倍软膏局部外用,观察其对二硝基氯苯丙酮溶液诱发变态反应和对磷酸组胺致痒反应的影响。结果中、高浓度的复方黄倍软膏能明显降低二硝基氯苯丙酮致小鼠超敏反应值(P<0.01),提高豚鼠耐受磷酸组胺的致痒阈(P<0.001)。结论复方黄倍软膏具有较好的抗过敏和止痒作用。     

人牙龈成纤维细胞系的建立及其生物学特性

目的：建立人牙龈成纤维细胞系并观察其生物学特性。方法：用组织块法培养细胞,用形态学、免疫组化染色、染色体分析等方法鉴定细胞,通过活细胞观察、生长曲线测定及ＭＴＴ比色实验研究细胞体外生长特性。结果：２０例原代培养,成活率９０％。培养细胞呈梭形,胞浆波形丝蛋白阳性,能合成Ⅰ型和Ⅲ型胶原及纤维粘连蛋白,具有正常二倍体核型,体外贴壁快,生长迅速,细胞寿命为（２５０．７±１１３．３）ｄ。结论：本实验建立的细胞系符合人牙龈正常二倍体成纤维细胞系。     

中药硬膏治疗肾阳亏虚型老年夜尿频25例临床体会

目的:观察外用中药硬膏治疗肾阳亏虚型老年夜尿频的临床疗效。方法:用中药硬膏热贴于关元穴、肾俞穴治疗肾阳亏虚型老年夜尿频。结果:25例患者总有效率为96%。结论:中药硬膏治疗肾阳亏虚型老年夜尿频效果理想,简便易行,临床推广使用。     

植物神经功能紊乱多汗症应用止汗散脐贴的临床观察及护理

[目的]探讨止汗散脐贴对植物神经功能紊乱多汗症的临床疗效及护理。[方法]将门诊194例植物神经功能紊乱多汗症患者随机分为两组,对照组82例基础护理及健康教育配合口服西药,实验组112例在基础护理及健康教育的基础上用止汗散穴位敷贴,5次为1疗程,共观察2个疗程。[结果]实验组总有效率为99.11%,显著高于对照组的82.93%。[结论]植物神经功能紊乱多汗症应用止汗散脐贴配合基础护理及健康教育取得良好的效果。其方法简单,疗效肯定,值得推广。