人牙龈成纤维细胞和牙周膜韧带细胞多向分化潜能的比较

目的 体外培养人牙周膜韧带细胞 （HPDLCs） 和人牙龈成纤维细胞 （HGFs）, 对比两者成骨、 成软骨以及成脂的多向分化潜能的差异。方法 运用酶消化结合组织块法体外培养 HPDLCs 和 HGFs, 选择生长状态良好的 3~4 代 HPDLCs 和 HGFs, 进行成骨、 成软骨、 成脂诱导, 未分化诱导的细胞作为对照组。分别用茜素红染色、 油红 O 染色以及阿利新蓝染色分别检测两种细胞的成骨、 成软骨及成脂分化能力。半定量逆转录聚合酶链反应 （RT-PCR） 检测 HPDLCs 和 HGFs 中相关标志基因骨钙素(OCN)、 Ⅰ型胶原蛋白 （Col 1）、 runt 相关转录因子 2 （RUNX2）、 过氧化物酶体增殖物激活受体γ2 （PPARγ2）、 X 型胶原蛋白 （Col 10） mRNA 的表达。结果 成骨诱导两种细胞培养至 28 d 时, 细胞周围均有红染钙结节形成, HPDLCs 形成的钙结节明显多于 HGFs; 成软骨诱导两种细胞 14 d 时均可见胞质蓝染的细胞, HGFs 较 HPDLCs 明显; 成脂诱导两种细胞 21 d 时, 可见红色脂肪滴形成, HPDLCs 形成的脂肪颗粒明显少于 HGFs。HGFs 和 HPDLCs 分化培养 7 d 和 14 d 后, 均有 OCN、 Col 1、 RUNX2、 PPARγ2 和 Col 10 的表达, 在 14 d 时的表达均高于 7 d; HPDLCs 中 OCN、 Col 1、 RUNX2 的表达高于 HGFs, PPARγ2、 Col 10 的表达低于 HGFs（均 P＜ 0.05）。结论 HPDLCs 的成骨能力较 HGFs 强, 成软骨和成脂能力较 HGFs 弱。     

Intrinsic activity and comparative molecular dynamics of buspirone analogues at the 5-HT(1A) receptors

In CNS, the 5-hydroxytryptamine(1A) (5-HT(1A)) receptors exist in two different populations with different behavioural and physiological effects: (1) somatodendritic autoreceptors located pre-synaptically of 5-HT containing neurons and (2) receptors located post-synaptic to 5-HT containing neurons. Clinical studies have shown that 5-HT(1A) partial agonists have anxiolytic properties, while antagonists of pre-synaptical autoreceptors shorten the onset time of selective serotonin reuptake inhibitors (SSRIs). In the present study, the pre- and post-synaptic activity of structural analogues of buspirone was evaluated in animal models. A three dimensional model of the 5-HT(1A) receptor was used to study their interaction modes and helical displacements upon receptor binding. The predicted receptor-ligand interactions indicated similarities in the receptor binding modes for all buspirone analogues, and no clear relationship between receptor contact residues and activity at pre- and post-synaptic receptors. Comparative molecular dynamics (MD) simulations for 650ps indicated that pre-synaptic antagonistic behaviour is connected to large displacements of transmembrane helix (TMH) 7 upon binding, while pre-synaptic agonistic behaviour is connected to large displacements of TMH2 and small displacements of TMH7. Post-synaptic partial agonist behaviour is connected to large displacements of TMH4 and TMH5 upon binding, while post-synaptic antagonists only slightly displace these helices.     

早产低体质量儿产妇在妊娠期的外周血、龈沟液炎性因子浓度对照分析

目的对照分析早产低体质量儿(PLBW)产妇在妊娠期外周血、龈沟液(GCF)炎性因子浓度分析。方法选择2013年3月—2014年1月在解放军第202医院妇产科住院分娩PLBW的产妇31例为观察组,对照组29例为同期住院、分娩正常体质量儿的产妇。两组对象均在分娩前接受了外周血及GCF中的炎性细胞因子(IL-1、IL-6和IL-10)检测。结果观察组产妇的外周血及GCF处的IL-1、IL-6浓度均明显高于对照组,而IL-10浓度则显著低于后者(P<0.05或P<0.01)。结论观察组产妇在妊娠期的外周血及GCF处的炎性细胞因子变化明显,应制定相应的口腔抗炎方案。     

Epithelial and connective tissue cell CTGF/CCN2 expression in gingival fibrosis

Gingival overgrowth is a side effect of certain medications and occurs in non-drug-induced forms either as inherited (human gingival fibromatosis) or idiopathic gingival overgrowth. The most fibrotic drug-induced lesions develop in response to therapy with phenytoin; the least fibrotic lesions are caused by cyclosporin A; and intermediate fibrosis occurs in nifedipine-induced gingival overgrowth. Connective tissue growth factor (CTGF/CCN2) expression is positively related to the degree of fibrosis in these tissues. The present study has investigated the hypothesis that CTGF/CCN2 is expressed in human gingival fibromatosis tissues and contributes to this form of non-drug-induced gingival overgrowth. Histopathology/immunohistochemistry studies showed that human gingival fibromatosis lesions are highly fibrotic, similar to phenytoin-induced lesions. Connective tissue CTGF/CCN2 levels were equivalent to the expression in phenytoin-induced gingival overgrowth. The additional novel observation was made that CTGF/CCN2 is highly expressed in the epithelium of fibrotic gingival tissues. This finding was confirmed by in situ hybridization. Real-time polymerase chain reaction (PCR) analyses of RNA extracted from drug-induced gingival overgrowth tissues for CTGF/CCN2 were fully consistent with these findings. Finally, normal primary gingival epithelial cell cultures were analysed for basal and transforming growth factor beta1 (TGF-beta1) or lysophosphatidic acid-stimulated CTGF/CCN2 expression at protein and RNA levels. These data indicate that fibrotic human gingival tissues express CTGF/CCN2 in both the epithelium and connective tissues; that cultured gingival epithelial cells express CTGF/CCN2; and that lysophosphatidic acid further stimulates CTGF/CCN2 expression. These findings suggest that interactions between epithelial and connective tissues could contribute to gingival fibrosis.     

热牙胶温度对AH-Plus根管糊剂所产生根尖微渗漏的影响研究

目的：分析温度对AH-Plus糊剂临床疗效的影响,为一次性根管治疗术中对温度的控制提供临床依据。方法：将48颗牙编号,按随机数字表法分为3组,采用AH-Plus糊剂在不同温度下对离体牙根管进行填充,使用染料渗入法检测微渗漏发生情况,用扫描电镜观察根管壁与封闭剂之间的结合情况。结果：1)在冠方微渗漏方面,不同温度下冠方微渗漏值均没有显著差异(P>0.05);2)在根尖微渗漏方面,C组(230℃)的根尖微渗漏显著小于A(150℃)、B组(180℃)(P<0.05),A、B两组的根尖微渗漏比较没有显著差异(P>0.05);3)扫描电镜对根尖区观察结果显示A组(150℃)的根管壁与牙胶之间缝隙明显大于B、C组。结论：在采用AH-PLUS热牙胶垂直加压技术充填根管时,设定热牙胶温度可能对根尖微渗漏产生影响,较高温度组(230℃)产生的根尖微渗漏小于较低温度组,冠方微渗漏无显著差异。     

Collagen fibril arrangement and size distribution in monkey oral mucosa

Collagen fibre organisation and fibril size were studied in the buccal gingival and hard palate mucosa of Macacus rhesus monkey. Light and electron microscopy analysis showed connective papillae exhibiting a similar inner structure in the different areas examined, but varying in distribution, shape and size. Moving from the deep to surface layers of the buccal gingival mucosa (free and attached portions), large collagen fibril bundles became smaller and progressively more wavy with decreasing collagen fibril diameter. This gradual diameter decrease did not occur in the hard palate mucosa (free portion, rugae and interrugal regions) where the fibril diameter remained constant. A link between collagen fibril diameter and mechanical function is discussed.