Laser Structured Dental Zirconium for Soft Tissue Cell Occupation—Importance of Wettability Modulation

Various approaches are being pursued to physico-chemically modify the zirconia neck region of dental implants to improve the integration into the surrounding soft tissue. In this study, polished zirconia discs were laser microstructured with periodic cavities and convex waves. These zirconia samples were additionally activated by argon plasma using the kINPen^®09. The surface topography was characterized by scanning electron microscopy and the surface wettability by water contact angle. The in vitro study with human gingival fibroblasts (HGF-1) was focused on cell spreading, morphology, and actin cytoskeleton organization within the first 24 h. The laser-induced microstructures were originally hydrophobic (e.g., 60 µm cavities 138.4°), but after argon plasma activation, the surfaces switched to the hydrophilic state (60 µm cavities 13.7°). HGF-1 cells adhered flatly on the polished zirconia. Spreading is hampered on cavity structures, and cells avoid the holes. However, cells on laser-induced waves spread well. Interestingly, argon plasma activation for only 1 min promoted adhesion and spreading of HGF-1 cells even after 2 h cultivation. The cells crawl and grow into the depth of the cavities. Thus, a combination of both laser microstructuring and argon plasma activation of zirconia seems to be optimal for a strong gingival cell attachment.     

Mucociliary function of the eustachian tube in the eustachian tube dysfunction

Background: Most of the tests to evaluate the eustachian tube (ET) function are focused on the ventilation function of the ET.

Aim: Here we evaluate mucociliary function of the ET in patients with ET dysfunction.

Materials and methods: Ten patients with ET dysfunction were enrolled into the study. Six patients had chronic tympanic membrane retraction and four patients had chronic middle ear effusion (MEE). All patients had intact tympanic membranes. Tympanometry and clinical examinations were done to all patients. Mucociliary function was evaluated with technetium labeled albumin and blue dye. Tympanometry and clinical examinations were done to six patients with chronic tympanic membrane retraction and four patients with chronic middle ear effusion (MEE). Mucociliary function of the ET was evaluated with technetium labeled albumin and blue dye placed into middle ear through an intact tympanic membrane and followed from nasopharynx ET orifice (blue dye) and with gamma camera (technetium).

Results: Blue dye was observed in tubal orifice in six (6 of 10) patients during 30?min observation. Five of those patients (5 of 6) had tympanic membrane retraction and one patient (1 of 6) had MEE. Tracer activity decreased from middle ear in six (6 of 10) patients. Four of those patients had tympanic membrane retraction and two had MEE.

Conclusion: Mucociliary function of the ET seems to be better in patients with tympanic membrane retraction than patients with middle ear effusion.

Significance: Mucociliary function of the ET is an important function for middle ear aeration, blue dye test is easily available to be used also in clinical practice.     

妇科膏方亦是美容佳品

膏方可活血通络,滋阴润肤,补益肝肾,调和气血,治病和养颜并举.女性有针对性地服用膏方,可以改善面部血液循环,调节女性逐渐衰退的内分泌功能,延缓女性的衰老,养颜靓肤.膏方美容作用稳定而且持久,宜于女性保养.     

Laboratory Test and Geochemical Modeling of Cement Paste Degradation,in Contact with Ammonium Chloride Solution

Concrete tanks, in coke wastewater treatment plants, are exposed to aggressive wastewater with high ammonium and chloride content, deteriorating the concrete binder. Due to this, toxic compounds may migrate to the environment. The results of the experimental work presented confirmed the changes in the phase, microstructure and concentration of chlorides caused by the penetration of NH₄Cl into the hardened cement paste in dry conditions. Geochemical modeling of the interactions between the aggressive solution, the cement stone matrix and the pore water was performed in order to track the destruction process effects. The results are useful for condition assessment of the structures operating under occasional immersion.     

Effect of vitamin E on periodontitis: Evidence and proposed mechanisms of action

BackgroundPeriodontitis is a noncommunicable inflammatory disease of the soft tissue and bone surrounding the teeth in the jaw, which affects susceptible individuals with poor oral hygiene. A growing interest has been seen in the use of dietary supplements and natural products for the treatment and prevention of periodontitis. Vitamin E consists of two major groups, namely tocopherols and tocotrienols, which are botanical lipophilic compounds with excellent anti-inflammatory and antioxidant properties.HighlightThis review aimed to summarize the preclinical and clinical findings on the effects of vitamin E on periodontitis. The current literature suggests that vitamin E could improve the periodontal status by correcting redox status imbalance, reducing inflammatory responses, and promoting wound healing, thus highlighting the potential of vitamin E in the management of periodontitis.ConclusionDirect evidence for the use of vitamin E supplementation or treatment of periodontitis in humans is still limited. More well-designed and controlled studies are required to ascertain its effectiveness.     

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目的    探讨烟草提取液（ST）和尼古丁液对人牙龈成纤维细胞（human gingival fibroblast,HGF）的影响。方法    2009年6—12月于昆明医学院口腔实验室,用含不同浓度的ST和尼古丁液的培养液体外培养HGF 5 d,应用噻唑蓝（MTT）快速比色法,检测细胞生长增殖情况。结果    与对照组相比,ST质量浓度从5 g／L开始时,抑制HGF的增殖,差异有统计学意义（P < 0.05）,随着浓度的加大,抑制作用更明显,呈浓度依赖性。同样,高质量浓度的尼古丁液从0.01 g／L开始对细胞增殖有抑制作用,并呈浓度依赖性,差异有统计学意义（P < 0.05）。结论    高浓度的ST和尼古丁均能抑制HGF的增殖,低浓度时则对细胞的增殖无影响。烟草对口腔黏膜上皮细胞的增殖呈现抑制作用,并随着浓度的加大,抑制作用愈发明显。     

壳聚糖-明胶-卡拉胶支架复合人牙龈成纤维细胞的体外研究

目的:研究人牙龈成纤维细胞(human gingival fibroblast cells,HGFs)在壳聚糖-明胶、壳聚糖-明胶-卡拉胶两种支架材料上生长增殖及某些细胞外基质分泌的情况,为研究组织工程化牙龈支架材料提供实验依据。方法:组织块法培养HGFs,将第4代的HGFs分别接种于两种支架材料上,以壳聚糖-明胶-卡拉胶为实验组,壳聚糖-明胶作为对照组。扫描电镜(SEM)观察两种支架材料的结构;四唑盐比色实验(MTT)法检测HGFs在两种支架材料上的增殖情况;通过酶联免疫吸附法(ELISA)检测细胞-支架复合培养液中I型胶原蛋白(Collagen I,Col I)和糖胺多糖(Glycosamlnoglycans,GAG)的含量。结果:扫描电镜显示两种支架材料均呈多孔结构,孔径50～200μm,孔隙率达90%。MTT法检测显示:培养1 d实验组与对照组吸光值(A值)差异无统计学意义(P>0.05);第3、5、7天时实验组A值明显高于对照组,差异有统计学意义(P<0.05)。ELISA结果显示,培养1 d实验组Col I和GAG的含量与对照组相比差异无统计学意义(P>0.05);第3、5、7天各时间点,实验组Col I和GAG的含量均明显高于对照组,差异有统计学意义(P<0.05)。结论:HGFs与壳聚糖-明胶-卡拉胶支架复合后,其生物学行为更为活跃,壳聚糖-明胶-卡拉胶支架比壳聚糖-明胶支架更适合作为组织工程化牙龈的生物支架材料。     

上下颌游离缺失Brnemark种植义齿早期临床效果的比较研究

目的:比较上下颌游离缺失Branemark种植义齿修复早期临床效果。方法 :5例上颌 (17颗 )和7例下颌(22颗 )游离缺失Branemark种植体植入金合金烤瓷桥修复行使功能18个月后 ,比较上下颌游离缺失Branemark种植体支持固定桥义齿的稳固情况、种植体周牙龈指数和种植体周骨吸收量。结果 :上下颌游离缺失Branemark种植体支持固定桥义齿种植体周骨吸收量无显著性差异 (P>0.05) ;两者种植体周牙龈均处于正常状态 ;上下颌游离缺失种植义齿均未见种植体松动或脱落 ,基台和全冠的稳固性均良好。结论 :上下颌游离缺失Branemark种植体支持种植固定桥义齿修复效果良好