Analysis of the association of IL1B (C+3954T) and IL1RN (intron 2) polymorphisms with dental implant loss in a Brazilian population

Background: Although dental implants have a high success rate, failures occur, in spite of adequate clinical conditions. Together with the observation that multiple implant losses occur in certain groups of individuals (clusterization phenomenon), this suggests that host response may influence implant failure. Little is known about the influence of genetic susceptibility on implant loss. Interleukin (IL)-1β and IL-1ra are believed to play a key role in the immune-inflammatory response, and polymorphisms IL1B (C+3954T) and IL1RN (intron 2) are shown to alter the coding proteins expression.
Objectives: The aim of this study was to investigate the association between dental implant loss and polymorphisms IL1B (+3954) and IL1RN (intron 2).
Material and methods: The study population ( n =266) was divided into Test group (T) – 90 subjects with implant loss, and Control group (C) – 176 subjects without any implant failure. Genotyping was performed by PCR-RFLP.
Results: The number of present teeth was observed to influence implant loss. No differences in genotype and allele frequencies between C and T were found for IL1B (+3954) and IL1RN (intron 2) polymorphisms. However, the analysis of the whole study population (control and test groups) showed that genotype 2/2 was significantly more frequent in individuals with multiple implant losses ( n =35) than in individuals that lost up to a single implant ( n =231) (OR: 3.07, IC: 1.13–8.34, P =0.027).
Conclusion: It was observed that number of teeth and edentulism were associated with implant loss. Genotype 2/2 of IL1RN polymorphism was significantly more frequent in patients who presented multiple losses, which suggests that the clusterization phenomenon has a genetic basis.     

Genetic screening in maternity care: preventive aims and voluntary choices

This paper analyses a prenatal genetic screening programme for three gene defects, conducted at maternity care centres in Eastern Finland in 1995 and 1996. What is special in this case is that the screening ended sooner than was planned. This paper investigates how the genetic screening programme was launched, the problems it encountered and the circumstances of its closure. The present analysis focuses on the co‐existence of, and tensions between, the two major objectives of the project: preventing disability and increasing mothers' choices. The tensions between these two objectives were highlighted at maternity care centres. The public health nurses working at these centres were confused about whether or not they should offer autonomous consumer choice or guidance to mothers to behave in responsible and health‐conscious ways. The nurses' confusion was intensified because the goal of prevention was identified with financial savings for the municipality, which the nurses thought was an inappropriate reason for abortions.     

VEGF基因多态性与肺癌危险因素的关系

背景与目的: 血管内皮生长因子(vascular endothelial growth factor,VEGF)是有力的血管生成介质,在肿瘤的生长及转移中起重要作用.本研究探讨VEGF基因多态性与肺癌危险因素的关系.方法: 以病例对照研究方法,采用PCR-RFLP技术,对171例肺癌患者和172例健康对照者的VEGF基因-2578C/A及936C/T位点基因型进行检测,明确两个位点基因型.并采用PHASE110软件构建这两个多态性位点的个体单倍体型.以非条件Logistic回归校正混杂因素,并进行多态性与肺癌风险关联性的统计学分析.结果: 携带至少1个-2578A等位基因的个体与携带-2578CC基因型的个体相比,肺癌发病风险降低(P=0.001,OR=0.303,95%CI0.153～0.601).性别分层分析显示:携带-2578CA AA基因型男性患者其肺癌发病风险降低.936C/-2578C与936C/-2578A两种单倍体分布差异有显著性(P=0.016,OR=0.317,95%CI 0.124～0.809:P=0.018,OR=0.547,95%CI 0.331～0.903).病理分层显示:与其他对照组单倍体相比,C-C单倍体个体其腺痛发病风险降低(P=0.004,OR=0.237,95%CI 0.090～0.627).结论: VEGF基因多态性是肺癌危险因素的风险因素.     

遗传算法优化尿样核苷毛细管电泳分析条件

目的:研究建立一种基于遗传算法的尿样核苷类成分的毛细管电泳条件优化方法。方法:用中心组成试验设计系统考察硼砂、SDS、pH 和电压等因素对尿样核苷类成分电泳分离结果的影响。采用色谱指数方程对分离结果进行评价,并将其作为适应度函数。运用实数编码的遗传算法进行全局寻优,获取最优分析条件。结果:在优化的分析条件(15.7 mmol·L~(-1)硼砂,250.0 mmol·L~(-1)SDS,pH 9.60,电压14.8 kV)下,各核苷类成分在12 min 内得到较好分离。结论:本文方法准确可靠,适用于毛细管电泳分析条件优化。     

MIBP1基因敲除载体的构建

目的:构建MIBP1基因敲除载体。方法:以小鼠129胚胎干细胞基因组DNA为模板,根据GenBank注册序列,扩增小鼠MBP-2基因启动子区5′端片段(5′arm)和内含子区3′端片段(3′arm),然后分别克隆进敲除载体pKOScramblerNTKV-1906,酶切鉴定后即得到MIBP1基因敲除载体pNTKV-3′,5′arm。用SalⅠ对pNTKV-3′,5′arm线性化,用于胚胎干细胞电转染。结果:成功扩增并克隆到小鼠129胚胎干细胞MBP-2基因的5′arm和3′arm片段,构建了MIBP1基因敲除载体。结论:成功构建了MIBP1基因敲除载体。     

硫嘌呤类药物遗传药理学研究进展

临床上硫嘌呤类药物应用广泛,但在此类药物的使用过程中,血液毒性、肝脏毒性、胰腺炎等不良反应的发生率较高。研究发现,硫嘌呤甲基转移酶(thiopurine S-methyltransferase,TPMT)的活性和遗传多态性,以及三磷酸肌苷焦磷酸酶(inosine triphosphate pyrophosphatase,ITPA)的遗传多态性与硫嘌呤类药物不良反应的发生密切相关。本文综述了TPMT活性和基因多态性,以及ITPA基因多态性的研究进展。     

IL-6与肥胖和胰岛素抵抗的关系

目的：探讨血清IL-6水平以及IL-6基因多态性与肥胖和胰岛素抵抗（IR）的关系。方法：选取肥胖2型糖尿病患者55例（肥胖组），非肥胖2型糖尿病患者49例（非肥胖组），健康对照50例（对照组），应用酶联免疫法检测血清中IL-6水平，并应用PCR-RFLP方法检测IL-6-174启动子区基因多态性。结果：肥胖组血清IL-6水平较非肥胖组及对照组明显增高．在糖尿病组中IL-6-174G等位基因携带者体质量指数（BMI）及IR均高于IL-6-174C等位基因携带者。结论：IL-6与BMI和IR有关，携带IL-6-174G等位基因者更易于发生IR。     

绿色荧光蛋白标记的人脂联素真核表达载体的构建

目的：构建绿色荧光蛋白（GFP）标记的人脂联素（Adiponectin）真核表达质粒。方法：用本实验室已构建好的人脂联素克隆载体pMD18—T／Adiponeetin为模板，结合已设计好的特异性引物。采用PCR方法克隆出Adiponectin目的片段，将该目的片段再连至真核表达载体pcDNA3．1／CT—GFP—TOPO上，转化入TOP10感受态细胞中，通过筛选得到融合有绿色荧光蛋白的重组质粒pcDNA3．1／CT—GFP—TOPO—Adiponectin。结果：PCR获得长度为736bp的目的片段，经pcDNA3．1／CT—GFP-TOPO真核表达载体连接、筛选及序列分析后，证实所插入的目的片段与genebank检索的人脂联素cDNA序列（Accession NM-004797）100％配。结论：绿色荧光蛋白标记的人脂联素真核表达载体构建成功。     

胆固醇酯转运蛋白基因TaqⅠ多态性与多廿烷醇降脂疗效的关系

目的:了解胆固醇酯转运蛋白(CETP)TaqⅠ基因多态性与新型降脂药物多廿烷醇(policosanol)疗效的关系。方法:入选54例LDL-C≥3.62 mmol.L-1,或TC≥6.2 mmol.L-1汉族原发性高胆固醇血症患者,服用10mg.d-1多廿烷醇12周,于0周和12周进行血脂检查;同时进行CETP TaqI位点多态性检测(PCR-RFLP方法)。结果:CETP基因TaqI位点B1B1,B1B2,B2B2基因型频率分别是31.48%,44.44%,24.07%。0周时apoA水平在3种基因型呈B1B1相似文献   

重组人白细胞介素15工程菌高密度发酵条件探讨

目的研究表达重组人白细胞介素15(rhIL-15)工程菌高密度发酵的条件。方法采用发酵罐发酵,对影响工程菌生长和目的蛋白质表达的因素进行优化。结果以甘油为碳源可明显抑制有害物质乙酸的积累,提高工程菌的表达量和收获率。高密度发酵明显提高了目的蛋白质的表达量和工程菌的产量。结论该发酵工艺大大提高了rhIL-15工程菌的产量和表达水平,为rhIL-15的规模化生产打下了基础。