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51.
根除幽门螺杆菌疗效与细胞色素氧化酶P450 2C19基因多态性的关系 总被引:3,自引:0,他引:3
目的 比较雷贝拉唑与奥美拉唑三联疗法根除幽门螺杆菌(Hp)的疗效与细胞色素氧化酶P4502 C19(CYP 2C19)基因多态性的关系。方法 采用随机、对照研究方法,将169例因消化不良症状接受常规胃镜检查确诊为慢性胃炎且Hp阳性的连续患者分人两组:雷贝拉唑三联疗法组(RAC组85例)和奥美拉唑三联疗法组(OAC组84例)。Hp诊断依靠组织病理学检查并参考快速尿素酶试验、血清Hp抗体检测结果。RAC组、OAC组均给予三联治疗:RAC组:雷贝拉唑10mg,OAC组:奥美拉唑20mg,两组均联用羟氨苄青霉素1000mg和克拉霉素500mg,全部药物每日2次,疗程7d。采用聚合酶链式反应结合限制性内切酶技术(PCR-RFLP),进行CYP 2C19基因型分析,治疗结束后第28天用^14C尿素呼气实验检测Hp根除疗效。结果 160例完成治疗方案,RAC组及OAC组的Hp根除率按PP分析及ITT分析均无统计学差异(P〉0.05)。根据CYP 2C19基因型分析,160例中,弱代谢型(PM)、中间代谢型(IM)及强代谢型(EM)的Hp根除率分别为95.5%(21/22)、85.9%(73/85)和67.9%(36/53)。PM型及IM型的Hp根除率均显著高于EM型(P〈0.05),而PM型与IM型间差异无统计学意义(P〉0.05)。RAC组中,各基因型的Hp根除率差异无统计学意义(P〉0.05)。OAC组中。IM型与EM型间(P〈0.01)及EM型与PM型间(P〈0.05)差异均有统计学意义。结论 雷贝拉唑与奥美拉唑两种三联疗法均能有效根除Hp。总疗效差异无统计学意义。雷贝拉唑三联疗法疗效较稳定,个体间差异小。PM型及IM型的Hp根除率均较EM型为高。 相似文献
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目的:介绍实验性小鼠膜性肾小球肾炎(MGN)的复制方法,并探讨其免疫荧光定量分析在肾小球肾炎研究中的应用价值。方法:制备阳离子化牛血清白蛋白(GBSA)并复制小鼠MGN,对各组小鼠进行电镜及免疫荧光观察,并进行免疫荧光定量研究。结果:电镜、免疫荧光观察均显示病理Ⅰ组(PⅡ组)具有典型的MGN病变,病理Ⅱ组(PⅡ组)病变轻微且不典型。免疫荧光定量研究证实PⅠ、PⅡ组与对照组差异有显著性;PⅠ、PⅡ组间差异无显著性。结论:C—BSA可作为复制小鼠MGN的良好抗原,隔日2mg/只尾静脉注射4w即可复制出稳定的小鼠MGN模型。免疫荧光定量分析不仅能直接而准确地反映MGN病理变化,而且在MGN早期即具有诊断价值。 相似文献
55.
目的对FKBP6基因第3、4外显子进行突变和多态性筛查,研究第3外显子278C/A位点及第2内含子C/T位点(rs7797242)在无精症患者和正常男性中的多态性,初步探讨与原发无精症的相关性。方法采用变性高效液相色谱和聚合酶链反应-限制性片段长度多态性方法,对第3、4外显子进行突变和多态性筛查,对177例无精症患者和231名正常男性的278C/A和C/T(rs7797242)多态性进行基因分型。结果278C和278A等位基因频率符合Hardy-Weinberg平衡。无精症患者278A显著低于正常对照,差异有统计学意义(P<0.05)。C/T多态性在两组中均未检出,第3、4外显子未筛查到新的变异。结论278A等位基因可能与原发无精症相关。C/T(rs7797242)及370G/A,430G/C,467T/C,468G/A在中国人群中非常罕见。 相似文献
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F. B. Hollinger 《Journal of viral hepatitis》2007,14(S1):11-15
Summary. Hepatitis B virus (HBV) circulates in blood as closely related, but genetically diverse molecules called quasispecies. During replication, HBV production may approach 1011 molecules/day, although during peak activity this rate may increase 100–1000 times. Generally, DNA polymerases have excellent fidelity in reading DNA templates because they are associated with an exonuclease which removes incorrectly added nucleotides. However, the HBV-DNA polymerase lacks fidelity and proofreading function partly because exonuclease activity is either absent or deficient. Thus, the HBV genome and especially the envelope gene, is mutated with unusually high frequency. These mutations can affect more than one open reading frame because of overlapping genes. The S gene contains an exposed major hydrophilic region (residues 110–155), which encompasses the 'a' determinant that is important for inducing immunity. Nucleotide substitutions in this region are common and result in reduced binding or failure to detect hepatitis B surface antigen (HBsAg) in diagnostic assays. Adaptive immunity also depends on the recognition of HBsAg by specific antibody and variants pose a threat if they interfere with binding to antibody. Finally, genomic hypervariability allows HBV to escape selection pressures imposed by antiviral therapies, vaccines and the host immune system, and is responsible for creating genotypes, subgenotypes and subtypes. 相似文献
58.
M van der Neut Kolfschoten R J Dirven S R Poort R van Wijk H L Vos F R Rosendaal R M Bertina 《Journal of thrombosis and haemostasis》2004,2(6):910-917
BACKGROUND: During the study of a family with hereditary factor (F)V deficiency (FV Amersfoort, 1102 A > T in exon 7) we identified an individual with 5% FV heavy chain antigen (FV(HC)) and 50% FV light chain antigen (FV(LC)). Further testing revealed that apart from the FV Amersfoort allele a second variant FV allele was segregating in this family, which encodes for a FV molecule with a reduced affinity for mAb V-23 used in the FV heavy chain ELISA (ELISA(HC)). OBJECTIVE: Identification and characterization of the molecular basis responsible for the reduced affinity of the variant FV for mAb V-23. METHODS: Family members of the proband were screened for mutations in the exons coding for the heavy chain of FV, after which the recombinant variant FV could be generated and characterized. Next, the cases and controls of the Leiden Thrombophilia Study (LETS) were genotyped for carriership of the variant FV. RESULTS: In the variant FV allele a polymorphism in exon 3 (409G > C) was identified, which predicts the replacement of aspartic acid 79 by histidin (D79H). Introduction of this mutation in recombinant FV confirmed that it reduces the affinity for binding to mAb V-23. The substitution has no effect on FV(a) stability and Xa-cofactor activity. In Caucasians the frequency of the FV-79H allele is approximately 5%. Analysis of the LETS revealed that the FV-79H allele is not associated with FV levels (FV(LC)), activated protein C sensitivity (using an activated partial thromboplastin time-based test) or risk of venous thrombosis (OR 1.07, CI 95: 0.7-1.7). CONCLUSION: The D79H substitution in FV should be considered as a neutral polymorphism. The monoclonal antibody V-23, which has a strongly reduced affinity for FV-79H, is not suitable for application in diagnostic tests. 相似文献
59.
The association of MTHFR and CBS variants with the doubling time and responsiveness to several chemodrugs was analyzed in 26 human cancer xenografts. The tumors homozygous for the absence of insertion (NN) for the CBS 844ins68bp were more chemosensitive than those with insertion (NI) to TS-1 (P=0.0048), suggesting a potential effect of this variant on fluoropyrimidine efficacy. Furthermore, the doubling time of tumors with a variant C allele (AC or CC) in MTHFR-A1298C was significantly longer than that of tumors with a normal allele (AA) (P=0.0008). Twenty-nine cellular proliferation-related genes were associated with MTHFR-A1298C genotyping and with the doubling time. 相似文献
60.
目的探讨体内外基因转移F as配体(F as-ligand,F asL)对恶性人黑素瘤细胞凋亡的影响。方法用携带人F asL cDNA的缺陷型重组腺病毒(A d-F asL),在体外转导两株黑素瘤细胞,并使其表达;通过流式细胞仪、RT-PCR法进行F as/F asL表达检测,TUNEL法及荧光显微镜相关凋亡检测、分析。建立人黑素瘤裸鼠模型,并对其进行体内疗效观察及病理学检查。结果流式细胞仪和RT-PCR检测两株黑素瘤细胞表面均表达F as,不表达F asL,而A d-F asL转导的两株黑素瘤细胞均能表达F asL;A d-F asL能显著诱导两株黑素瘤细胞在体外凋亡或抑制其生长。体内疗效观察黑素瘤荷瘤鼠模型治疗组瘤重(0.48±0.16)g与对照组瘤重(1.02±0.19)g相比,差异有显著性(P<0.05)。肿瘤组织形态学检查,治疗组可见肿瘤细胞凋亡坏死区及炎性细胞浸润。结论F asL基因重组腺病毒在体内外均具有显著诱导人黑素瘤细胞凋亡的效果。 相似文献