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61.
The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-gamma) production by murine splenocytes
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The acidic ribosomal proteins of the protozoan parasite Leishmania infantum have been described as prominent antigens during both human and canine visceral leishmaniasis. In this study we present data showing that the intraperitoneal administration in BALB/c mice of the Leishmania LiP2a protein, in the absence of any added adjuvants, elicited a strong humoral response as an indication that the protein is a potent immunogen. Despite the evolutionary conservation of the acidic ribosomal proteins, the antibody response was found to be specific for the Leishmania protein. Another remarkable finding was the observation that the LiP2a protein stimulates the in vitro proliferation of splenocytes from either LiP2a-immunized or naive BALB/c mice. Since similar proliferative indices were observed in T cell-enriched cultures, it is likely that the LiP2a stimulating activity is due mainly to T lymphocyte expansion. Also, the stimulatory effect was demonstrated to be antigen-specific, since the proliferation was abrogated by the presence of anti-LiP2a antibodies. Interestingly, the LiP2a protein stimulated the production of substantial amounts of IFN-gamma in cultured splenocytes from LiP2a-immunized mice. Our data indicate therefore that the immunostimulatory properties shown by this antigen should be taken into account when developing therapeutic and prophylactic vaccines against leishmaniasis. 相似文献
62.
目的:观察低剂量γ射线照射人离体外周血对血清中一氧化氮(NO)和一氧化氮合酶(NOS)水平的影响。方法:采集10份健康献血员全血,肝素抗凝,然后采用γ射线照射,照射剂量率为17Gy/min,总吸收剂量为1Gy,分别于照射前及照射后1h,2h采用分光光度法检测血清中NO含量和NOS活性。结果:经γ射线照射1h后,血清中NO及NOS水平与照射前比较明显升高(P〈0.01);照射后2h,血清中NOS水平与照射后1h比较无统计学差异(P〉0、05),但是还明显高于照射前的水平(P〈0.01);在照射后2h,血清中NO含量与照射后1h比较明显降低(P〈0.01),但仍明显高于照射前水平(P〈0.01)。结论:采用剂量为1Gy的γ射线照射外周血,可引起血清中NO水平及NOS活性的显著升高.从而为低剂量辐照自体血回输对肿瘤的辅助治疗提供了理论支持。 相似文献
63.
We have previously demonstrated that the transformation of the caudal spinal cord through the conus medullaris to the filum terminale takes place in three steps. In the conus medullaris the twin layers of CGRP-immunoreactive and IB4-labeled primary afferent fibers as well as the translucent portion of the superficial dorsal horn equivalent to the substantia gelatinosa discontinue before the complete removal of the dorsal horn. Parallel with these changes VGLUT1-immunoreactive myelinated primary afferent fibers arborize not only in the deep layers but also in the entire extension of the remaining dorsal horn, while scattered CGRP fibers still remains at the margin of and deep in the dorsal horn. PKCgamma-immunoreactive dorsal horn neurons discontinue parallel with the disappearance of the IB4-labeled nerve fibers. These observations suggest that in the dorsal horn certain neurons are linked to the substantia gelatinosa, while others are substantia gelatinosa-independent neurons. 相似文献
64.
Basophil leukocytes obtained from AIDS patients, allergic patients and healthy controls were stimulated in vitro with interleukin 4, lymphotoxin, tumour necrosis factor alpha and interferon gamma to examine the histamine releasing effect. The cytokines caused histamine release from the basophils of approximately half the AIDS patients and from 8-17% of the allergic patients. No response was obtained in the control group. Removal of cell surface immunoglobulins abolished the response to cytokines, indicating an Ig-dependent mechanism. Passive sensitization with cell-derived Ig, with Ig deprived of IgE, or with IgG, indicated that cell-bound IgE was responsible for the cytokine-induced histamine release in AIDS patients. This response may be mediated by cytokine-selective IgE antibodies. 相似文献
65.
Naohiro Yano Masayuki Endoh Raita Naka Fumio Takemura Yasuo Nomoto Hideto Sakai 《Journal of clinical immunology》1996,16(1):71-79
Previously we reported disease-specific interaction between interferon-gamma" align="MIDDLE" BORDER="0"> (IFN-gamma" align="MIDDLE" BORDER="0">) and interleukin-4 (IL-4) in patients with IgA nephropathy (IgAN), suggesting the existence of unusual T cell behavior in this disease. In the present study, we investigated characteristic synthesis of interferon-gamma" align="MIDDLE" BORDER="0"> (IFN-gamma" align="MIDDLE" BORDER="0">) and expression of IFN-gamma" align="MIDDLE" BORDER="0"> receptor (IFN-gamma" align="MIDDLE" BORDER="0">R) in the peripheral blood mononuclear cells (PBMC) from patients with IgAN and other chronic proliferative glomerulonephritis (PGN). Heparinized peripheral blood samples were obtained from 38 patients with chronic mesangial proliferative glomerulonephritis (CGN; including 24 with IgA nephropathy) and 20 healthy controls. PBMC were isolated by gradient centrifugation and fragments were cultured in Iscove's modified Dulbecco's medium (IMDM) supplemented with 10% fetal calf serum (FCS) for 72 hr. IFN-gamma" align="MIDDLE" BORDER="0"> concentrations in supernatants were evaluated by the enzyme-linked immunosorbent assay (ELISA). Other parts of PBMC pellets were reacted with anti-human IFN-gamma" align="MIDDLE" BORDER="0">R monoclonal antibody and FITC-labeled anti-mouse second antibody for analysis of IFN-gamma" align="MIDDLE" BORDER="0">R expression on these cells by FACScan. The remaining PBMC were fractionated into CD4+ T cells, CD8+ T cells, B cells, NK, cells and macrophages using the MACS cell sorting system. The isolated cells were evaluated for IFN-gamma" align="MIDDLE" BORDER="0"> or IFN-gamma" align="MIDDLE" BORDER="0">R mRNA expression by the semiquantitative RT-PCR method.In vitro IFN-gamma" align="MIDDLE" BORDER="0"> synthesis was enhanced in patients with CGN, and NK cells were revealed to be responsible for such enhancement. On the other hand, the expression of IFN-gamma" align="MIDDLE" BORDER="0">R on macrophages was suppressed in CGN patients. These results suggest that impairment of regulation of the IFN-gamma" align="MIDDLE" BORDER="0"> system might be involved in the development of CGN. 相似文献
66.
Csiszár A Nagy G Gergely P Pozsonyi T Pócsik E 《Clinical and experimental immunology》2000,122(3):464-470
67.
Tartour Eric; de la Salle Henri; de la Salle Corinne; Teillaud Christophe; Camoin Luc; Galinha Annie; Latour Sylvain; Hanau Daniel; Fridman Wolf H.; Sautes Catherine 《International immunology》1993,5(8):859-868
Low affinity Fcgamma.gif" ALT="{gamma}" BORDER="0">R are a heterogeneous group of glycoproteinswhich exist in transmembrane (TM) as well as in soluble forms.Two membrane isoforms of the murine type II Fcgamma.gif" ALT="{gamma}" BORDER="0">R, Fcgamma.gif" ALT="{gamma}" BORDER="0">Rilb1 andFcgamma.gif" ALT="{gamma}" BORDER="0">;Rilb2, have been described. They result from the translationof alternatively spliced premRNA, Fcgamma.gif" ALT="{gamma}" BORDER="0">Rilb2 lacking sequencesof the first intracytoplasmic domain (IC1). Soluble forms ofFcgamma.gif" ALT="{gamma}" BORDER="0">R (sFcgamma.gif" ALT="{gamma}" BORDER="0">R) have previously been shown to result from proteolysisof membrane receptors. We report here the identification, inmacrophages, of a mRNA derived from the FCgamma.gif" ALT="{gamma}" BORDER="0">Rll gene by splicingexons encoding the TM and IC1 domains, i.e. corresponding toa TM-deleted Fcgamma.gif" ALT="{gamma}" BORDER="0">Rllb2 mRNA. A soluble protein possibly encodedby this mRNA was identified in macrophage supernatants. In accordancewith Fcgamma.gif" ALT="{gamma}" BORDER="0">R nomenclature, we propose to name this new Fcgamma.gif" ALT="{gamma}" BORDER="0">Rll IsoformFcgamma.gif" ALT="{gamma}" BORDER="0">Rllb3. It is the most abundant 8Fcgamma.gif" ALT="{gamma}" BORDER="0">R present in serum, as comparedwith 8Fcgamma.gif" ALT="{gamma}" BORDER="0">R resulting from cleavage of membrane Fcgamma.gif" ALT="{gamma}" BORDER="0">R. 相似文献
68.
Two cytokines IL-4 and IL-12 are known to determine the balance between T(h)1 and T(h)2 development. In addition to IL-4 production of V(alpha)14(+) NKT cells, they have recently been demonstrated to have the capacity to stimulate IL-12 production by antigen-presenting cells (APC). This study demonstrates that IFN-gamma is absolutely required for the NKT cell-stimulated IL-12 production. Culture of B cell-depleted spleen cells from C57BL/6 mice with alpha-galactosylceramide (alpha-GalCer) capable of selectively stimulating V(alpha)14/J(alpha)281(+) NKT cells resulted in the production of IL-12 together with IL-4. Whereas IL-4 production occurred in culture of IFN-gamma(-/-) C57BL/6 splenocytes, the same culture failed to generate IL-12 production. While IL-12 production induced during culture of V(alpha)14(+) NKT cells and APC depended on the interaction between CD40 ligand on NKT cells and CD40 on APC, the expression levels of these key molecules were comparable in cells from wild-type and IFN-gamma(-/-) mice. Addition of rIFN-gamma to alpha-GalCer stimulated IFN-gamma(-/-) splenocyte culture, and administration of rIFN-gamma to alpha-GalCer-injected IFN-gamma(-/-) mice resulted in the restoration of IL-12 production in vitro and in vivo. These results illustrate a mandatory role for IFN-gamma in V(alpha)14(+) NKT cell-stimulated IL-12 production by APC. 相似文献
69.
Kovalenko P Fujinaka H Yoshida Y Kawamura H Qu Z El-Shemi AG Li H Matsuki A Bilim V Yaoita E Abo T Uchiyama M Yamamoto T 《International immunology》2004,16(5):625-634
Anti-glomerular basement membrane (GBM) glomerulonephritis induced in WKY rats is characterized by glomerular accumulation of CD8(+) T cells and monocytes/macrophages, followed by crescent formation. The mechanism of leukocyte accumulation after antibody binding to GBM is still unclear. To unveil an involvement of Fcgamma receptors (FcgammaR) in leukocytes recruitment we examined the expression of FcgammaR in glomeruli and the effects of the administration of F(ab')(2) fragment of anti-GBM antibody or FcgammaR blocking on the initiation and progression of this model. A gradual increase of FcgammaR mRNA expression in glomeruli during the time course of disease suggested their significance in the development of glomerulonephritis. Glomerular lesions and proteinuria were induced only in rats injected with intact IgG of anti-GBM antibody, but not with the F(ab')(2) fragment. In vivo blocking of FcgammaR by administering heat-aggregated IgG led to the decrease of mRNA expression for all types of FcgammaR (types 1, 2 and 3) and a significant amelioration of glomerulonephritis manifestations. By flow cytometry and immunohistochemistry FcgammaR2-expressing cells in glomeruli were identified as macrophages, but not CD8(+) T cells. The expression of FcgammaR1 and 3 was significantly decreased, and that of FcgammaR2 became undetectable in CD8(+) T cell-depleted rats. Thus, CD8(+) T cells may stimulate FcgammaR expression on macrophages, contributing to their glomerular accumulation and injury. These studies provide direct evidence for a crucial involvement of IgG Fc-FcgammaR interaction in glomerular recruitment of macrophages and following induction of anti-GBM glomerulonephritis in WKY rats. 相似文献
70.