新的抗癌中药枫苓合剂的主要药效学

目的研究枫苓合剂体内抗癌作用及其他有关药理,为临床试验提供基础。方法采用对人体肿瘤移植的裸鼠体内人体胃癌MKN及人体肝癌QGY的抑制,对荷瘤和正常动物免疫功能的影响及与化学合成药合并用药等方面进行枫苓合剂的主要药效学研究。结果枫苓合剂对人体胃癌MKN 3个剂量组口服给药抑癌率分别为:74．68％-86．76％,49．86％- 59．31％及19．12％-21．68％;腹腔给药抑癌率分别为:78．72％-85．42％,55．32％-62．50％及26．69％-41．67％。对肝癌QGY口服给药抑癌率分别为:47．69％-49．33％,31．94％-33．63％及20．0％-26．0％。对荷Lewis肺癌小鼠显示提高机体NK细胞活力,有明显促进小鼠腹腔巨噬细胞的吞噬功能。对S180肉瘤的生长抑制,单独用环磷酰胺(CTX)15 mg·kg-1抑瘤率为45．16％,与枫苓合剂25 mL·kg-1合并用药抑瘤率为71．29％。结论高剂量枫苓合剂具有高的抗胃癌MKN的作用和中度的抗肝癌QGY的药效,且可提高免疫力,与CTX合用有增效作用。     

CIK和CEA负载DC联合化疗治疗胃癌的研究

目的 评价胃癌患者术后应用CIK和CEA负载DC联合化疗的临床疗效.方法 选取行胃癌根治手术治疗的患者30例,随机分为2组,分别给予化疗和CIK-DC/CEA细胞免疫治疗联合化疗的治疗方案,随后观察治疗之后的临床反应,并随访其临床疗效、生活质量情况及生存率等评价指标.结果 免疫细胞回输治疗后,患者未见明显不良反应,血清中Th1类细胞因子水平较治疗前有明显升高,治疗前后比较有显著性差异（P〈0.05）;随访结果显示,免疫治疗临床疗效、生活质量、生存率较对照组均有一定的改善,但无显著性差异（P〉0.05）.结论 CIK-DC/CEA细胞免疫治疗胃癌是有一定的近期临床疗效的,可为改善胃癌根治术的预后以及免疫治疗提供一定的理论基础.     

手十二井穴刺络放血法对MCAO模型大鼠大脑皮层c-fos蛋白表达的影响

目的 从脑内c-fos蛋白表达的角度观察井穴放血法对急性缺血性脑损伤的脑保护作用。方法 以凝结法阻断大鼠一侧大脑中动脉造成急性局灶性缺血性脑损伤模型(MCAO)，用免疫组织化学法观察缺血区皮层脑组织c-fos阳性细胞的表达变化及施加手十二井穴放血法后对其的干预治疗作用。结果 急性缺血性脑损伤可快速诱导c-fos蛋白在皮层缺血区脑组织的表达，其表达有随缺血时间延长逐渐增多的趋势，而缺血后即刻施加井穴放血的干预治疗．在1h、3h、6h、24h各缺血时间段均可明显上调缺血区皮层脑组织c-fos蛋白的表达水平。结论 c-fos蛋白表达既与缺血的严重程度有关．也反应神经元的应激反应能力，脑缺血能诱导c-fos蛋白的逐渐表达，提示脑的缺血性损伤可应激性地增强神经细胞对缺血、缺氧的耐受和适应能力。而早期施加井穴刺络放血疗法能进一步增强c-fos蛋白表达，提高神经细胞的应激能力，从而提高缺血区脑组织的抗损伤修复能力，减缓神经细胞凋亡，抵抗缺血性脑损害的进一步发展，产生有效的脑保护作用，表明井穴放血法对中风早期有一定的急救作用。     

基于无尺度网络法的4 116 张胃癌中医处方研究

本研究借助临床科研信息共享系统,使用无尺度网络挖掘方法,分析了江苏省中医院门诊994 例胃癌患者中医治疗的4 116 张处方,以图示化直观展示了核心药物及配伍,揭示出该院胃癌的中医治疗处方用药特点。该方法具有较高的可信度,对于中医处方的研究具有一定的意义和价值。     

Association between the Dietary Inflammatory Index and Gastric Disease Risk: Findings from a Korean Population-Based Cohort Study

Evidence suggests that diets with high pro-inflammatory potential may play a substantial role in the origin of gastric inflammation. This study aimed to examine the association between the energy-adjusted dietary inflammatory index (E-DII^TM) and gastric diseases at baseline and after a mean follow-up of 7.4 years in a Korean population. A total of 144,196 participants from the Korean Genome and Epidemiology Study_Health Examination (KoGES_HEXA) cohort were included. E-DII scores were computed using a validated semi-quantitative food frequency questionnaire. Multivariate logistic regression and Cox proportional hazards regression were used to assess the association between the E-DII and gastric disease risk. In the prospective analysis, the risk of developing gastric disease was significantly increased among individuals in the highest quartile of E-DII compared to those in the lowest quartile (HR_quartile4vs1 = 1.22; 95% CI = 1.08–1.38). Prospective analysis also showed an increased risk in the incidence of gastritis (HR_quartile4vs1 = 1.19; 95% CI = 1.04–1.37), gastric ulcers (HR_quartile4vs1 = 1.47; 95% CI = 1.16–1.85), and gastric and duodenal ulcers (HR_quartile4vs1 = 1.46; 95% CI = 1.17–1.81) in the highest E-DII quartile compared to the lowest quartile. In the cross-sectional analysis, the E-DII score was not associated with the risk of gastric disease. Our results suggest that a pro-inflammatory diet, indicated by high E-DII scores, is prospectively associated with an increased risk of gastric diseases. These results highlight the significance of an anti-inflammatory diet in lowering the risk of gastric disease risk in the general population.     

Nutritional Status and Related Factors in Patients with Gastric Cancer after Gastrectomy: A Cross-Sectional Study

Patients after gastrectomy for gastric cancer are at risk of malnutrition, and poor nutritional status negatively affects patients’ clinical outcomes. Knowledge of the factors influencing patients’ nutritional status can inform interventions for improving patients’ nutrition. A cross-sectional study was conducted to describe nutritional status and related factors in gastric cancer patients after gastrectomy. A convenience sample of gastric cancer patients with gastrectomy was recruited from general surgery or oncology clinics of a medical center in northern Taiwan. Data were collected with self-reported questionnaires, including the Functional Assessment Cancer Therapy—Gastric Module version 4, the Concerns in Meal Preparation scale, the Center for Epidemiologic Studies Depression Scale, and the Mini Nutrition Assessment. One hundred and one gastric cancer patients participated in the study. There were 81 cases of subtotal gastrectomy and 20 cases of total gastrectomy. Most patients (52.5%) were malnourished or at risk. Linear regression showed that symptom severity (β = −0.43), employment status (β = 0.19), and difficulty in diet preparation (β = −0.21) were significant predictors of nutritional status. Together, these three variables explained 35.8% of the variance in patient nutritional status (F = 20.3, p < 0.001). More than 50% of our participants were malnourished or at risk for malnutrition, indicating a need for continued monitoring and support after discharge from hospitals. Special attention should be given to patients with severe symptoms, unemployment, and difficulties in diet preparation.     

阴式子宫瘢痕妊娠病灶清除术的临床疗效评价

目的 观察阴式子宫瘢痕妊娠病灶清除术治疗剖宫产术后瘢痕妊娠的意义.方法 选取我院2018年10月—2019年10月收治的64例剖宫产术后瘢痕妊娠患者的临床资料,根据手术方案的不同,将实施阴式子宫瘢痕妊娠病灶清除术的病例作为观察组(n=33),将选择腹腔镜子宫瘢痕妊娠病灶消除术的病例作为对照组(n=31).比较两组手术疗...     

Decreased expression of claudin-18.2 in alpha-fetoprotein-producing gastric cancer compared to conventional gastric cancer

BackgroundAlpha-fetoprotein-producing gastric cancer (AFPGC) is a subtype of gastric cancer (GC) with more aggressive biological behavior. As a highly specific tight junction component exclusively present in gastric mucosa and gastric adenocarcinomas, claudin-18.2 (CLDN18.2) has become an emerging target in GC. In this study, we aimed to provide insight into AFPGC and investigate the expression and the clinical implications of CLDN18.2 in AFPGC.MethodsWe retrospectively collected 98 cases of AFPGC and reviewed their clinical, morphological, and immunohistochemical features. Another 356 patients with stage-matched conventional GC (cGC) were enrolled as a control group. We further surveyed CLDN18.2 expression by immunohistochemistry (IHC) in 51 AFPGC tissues and explained its association with the clinicopathological parameters of AFPGC.ResultsOur results showed that AFPGC was a unique GC type with elevated serum alpha-fetoprotein (AFP), which was a predictor of a worse prognosis. AFPGC showed typical morphological features and positive staining of at least 1 hepatocytic or enteroblastic marker. The expression rate of CLDN18.2 was low, with a positivity rate of 21.6%, which was much lower than that observed in cGC tissues (38.5%). A significant correlation was found between CLDN18.2 expression and the differentiation of AFPGC. CLDN18.2 expression was negatively correlated with the serum AFP level of AFPGC. We also found that AFPGC with a hepatoid type (HPT) component showed a significantly lower CLDN18.2 expression than those without.ConclusionsThis study demonstrated that CLDN18.2 was significantly decreased in AFPGC and was negatively correlated with the patient’s preoperative serum AFP level. The negative correlation between AFP and CLDN18.2 could be explained by retro-differentiation of AFPGC. Special treatment strategies might be needed for this unique tumor type.     

Abrogation of ARF6 in promoting erastin-induced ferroptosis and mitigating capecitabine resistance in gastric cancer cells

BackgroundADP ribosylation factor 6 (ARF6) is a member of the Rat sarcoma virus (RAS) superfamily that is involved in the regulation of vesicular trafficking, membrane lipid remodeling, and signaling pathways. Our earlier work discovered that ARF6, as a downstream effector of the Kirsten rat sarcoma viral oncogene (Kras)/extracellular signal-regulated kinases (ERK) signaling pathway, may increase proliferation and induce the Warburg effect in gastric cancer (GC) cells. Additionally, ARF6 appears to be a potential biomarker for predicting the prognosis of GC. Ferroptosis has recently been described as a type of nonapoptotic iron-dependent cell death that is strongly associated with the Kras mutation. Therefore, it is critical to continue investigating the link between ARF6 and ferroptosis.MethodsWe first created ARF6 silenced cancer cell lines with lentivirus transfection. The knockdown efficiency was confirmed through quantitative polymerase chain reaction (qPCR) and western blotting. Subsequently, we used Cell Counting Kit-8 (CCK-8) and malondialdehyde (MDA) assay for lipid peroxidation measurement. Following this, qPCR and western blotting were conducted to clarify the mechanism involved. Finally, immunohistochemistry was used to stain human GC samples.ResultsOur findings established that, whereas ARF6 did not directly regulate lipid peroxidation, it did render GC cells susceptible to oxidative stress, particularly erastin-induced lipid peroxidation. Additionally, our research demonstrated that ARF6 may control capecitabine resistance via several routes.ConclusionsARF6 may play a critical role in the development of GC.