金骨莲胶囊HPLC指纹图谱及化学模式识别研究

目的建立金骨莲胶囊HPLC指纹图谱,并结合化学模式识别方法对其进行质量一致性评价,为该制剂的质量控制提供参考。方法采用ACE Excel 5 C18-PFP(250 mm×4.6 mm,5μm)色谱柱进行检测;流动相为乙腈-0.1%磷酸水溶液,进行梯度洗脱;体积流量为0.8 mL/min;柱温为40℃;检测波长为210 nm;进样量为10μL;建立12批金骨莲胶囊指纹图谱。对12批金骨莲胶囊指纹图谱进行相似度评价、层次聚类分析(hierarchical clustering analysis,HCA)和主成分分析(PCA),并结合正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)寻找不同批次金骨莲胶囊的差异成分。结果建立的金骨莲胶囊HPLC指纹图谱共标定了19个共有峰,其中2、8、11号峰归属于汉桃叶,5、7、10、12、15号峰归属于大血藤,16、17、19号峰归属于透骨香,1号峰为5味药材共有,3号峰归属于八角枫和大血藤,13号峰归属于大血藤和透骨香,4、9、14、18号峰归属于大血藤和汉桃叶,6号峰归属于大血藤、汉桃叶和透骨香。通过与对照品对比指认出2、3、6、7、9、12、13、15、16号峰分别为富马酸、没食子酸、原儿茶酸、红景天苷、绿原酸、香草酸、表儿茶素、鹅掌楸苷、滇白珠苷A。12批金骨莲胶囊指纹图谱相似度均在0.910以上,HCA、PCA 2种分析方法均把样品分为了3类,结合OPLS-DA筛选出了导致各批次之间产生差异的7个差异性标志物,分别为15(鹅掌楸苷)、14、6(原儿茶酸)18、16(滇白珠苷A)、19、9(绿原酸)号色谱峰。结论该分析方法简单可行、具有良好精密度、重复性和稳定性,建立的指纹图谱可为金骨莲胶囊的质量评价提供参考。     

豆蔻属药用植物叶绿体基因组密码子偏性分析

目的明确豆蔻属药用植物叶绿体基因组蛋白编码基因密码子使用模式及影响因素。方法以草果Amomum tsao-ko、阳春砂Amomum villosum、白豆蔻Amomum kravanh和爪哇白豆蔻Amomum compactum叶绿体基因组50个共有蛋白编码基因为材料,利用CodonW 1.4.2软件、CUSP在线程序和Excel软件等分析基因密码子使用偏性参数和核苷酸组成。结果 4个豆蔻属物种叶绿体基因组具有相似的密码子使用模式,密码子第3位碱基GC含量为37.22%～37.31%,偏向使用NNA和NNT型密码子;4个物种的有效密码子数值(ENC)均在35以上,表明豆蔻属叶绿体基因组密码子偏性较弱。综合中性分析、有效密码子数(ENC-plot)分析、奇偶偏好性(PR2-plot)分析和对应性分析的结果显示豆蔻属叶绿体基因组密码子偏性主要受选择影响,同时突变等其它因素也对密码子的偏性产生一定的作用。在草果、阳春砂、白豆蔻和爪哇白豆蔻中分别确定了18、16、16、18个最优密码子,其中共有最优密码子13个。结论豆蔻属植物叶绿体基因组密码子第3位碱基偏向使用A/T结尾,密码子偏性受选择影响最大,同时还受到诸如突变等其它因素的影响。     

山豆根的化学成分和药理作用的研究进展

山豆根Sophorae Tonkinensis Radix et Rhizoma用药历史悠久,为临床常用药,具有清热解毒、消肿利咽的功效。目前已从山豆根中鉴定出200多种化合物,主要含黄酮类、生物碱类、三萜类等,其中生物碱为山豆根主要活性成分。山豆根作用广泛,对免疫系统、消化系统、循环系统等均有调节作用,具有抗肿瘤、抗炎、护肝、降压、提高免疫、解痉止泻、升血糖等诸多药理作用。综述了山豆根的化学成分和主要药理作用,为山豆根的深入研究提供参考。     

Doppler Waveforms and Blood Flow Parameters of the Superior and Inferior Mesenteric Arteries in Patients Having Behçet Disease With and Without Gastrointestinal Symptoms

Objective . To evaluate hemodynamic changes in mesenteric arteries in patients with Behçet disease with and without gastrointestinal symptoms. Methods . Doppler sonography of mesenteric arteries was performed in 25 symptomatic and 15 asymptomatic patients having Behçet disease and in 25 healthy control subjects. The peak systolic, minimal, and mean velocities, resistive and pulsatility indexes, inner diameter, cross‐sectional area, and blood flow volume of mesenteric arteries were evaluated. The results were compared between patient groups and controls. Results . The mesenteric artery flow was significantly greater in patients in the symptomatic group than in those in the asymptomatic group or in controls. In the superior mesenteric artery, mean velocity and mean blood flow volume (0.35 ± 0.18 m/s and 711 ± 404 mL/min, respectively; P < .0001) in the symptomatic group were significantly higher than in the asymptomatic group (0.16 ± 0.07 m/s and 305 ± 168 mL/min, respectively) or in controls (0.15 ± 0.07 m/s and 290 ± 123 mL/min, respectively). The mean peak systolic velocity (1.23 ± 0.47 m/s; P < .005) in the symptomatic group was significantly higher than in controls (0.93 ± 0.23 m/s). In the inferior mesenteric artery, mean velocity and mean blood flow volume (0.25 ± 0.10 m/s and 139 ± 79 mL/min) in the symptomatic group were significantly higher than in the asymptomatic group (0.16 ± 0.07 m/s; P < .006; and 78 ± 26 mL/min; P < .007) or in controls (0.17 ± 0.07 m/s; P < .003; 83 ± 48 mL/min; P < .004). Conclusions . In this study, symptomatic patients with gastrointestinal Behçet disease were associated with a significant increase in mesenteric artery flow that could be evaluated easily on spectral patterns of arteries during Doppler sonography. The Doppler sonographic findings also revealed that intestinal involvement in patients with Behçet disease without gastrointestinal symptoms is not significantly different from that of healthy controls.     

Dermatoses professionnelles et aldéhydes

Occupational skin diseases (irritation, ulceration, urticaria and immunological eczema) to aldehydes, especially formaldehyde, glutaraldehyde and glyoxal, have been recognized for a long time in France as occupational diseases entitled to compensation. The occupational circumstances where these conditions are encountered are primarily in health care workers, particularely those who sterilize endoscopy, dental and dialysis instruments, who conserve tissues (especially in anatomy and pathology laboratories), those who use cooling fluids during machining of metals, who handle plastics containing formol derivatives, as well as those who have contact with certain textiles, cosmetics or nails hardeners. These allergens are very frequently encountered (formol is included in the standard battery of contact allergens), but it is expected that their use will decrease because of the now-recognized cancerogenic character of formol and the inefficiency of glutaraldehyde in sterilization against prions. Preventive measures against these aldehydes are therefore essential.     

Intracardiac thrombus and pulmonary artery aneurysm in Behcet's disease

Intracardiac thrombus is a rare manifestation of Behcet's disease. OBSERVATION: we report the case of a 60 year old male patient with Beh?et's disease who presented with dyspnea and haemoptysis. CT angiography and echocardiography was performed allowing the identification of right heart thrombus associated with pulmonary artery aneurysm. The outcome was favourable with medical management. CONCLUSION: Intracardiac thrombus is a rare complication of Behcet's disease, but the diagnosis should be sought early in order to allow medical management to be considered.     

Efficacy of infliximab in refractory posterior uveitis in Behcet's disease patients

Aim of the work

Ocular manifestations are the main cause of morbidity in Behcet's disease (BD). Infliximab (IFX), a chimeric monoclonal antibody directed against tumor necrosis factor-alpha, may be efficient in refractory uveitis due to BD. The aim of this study was to assess the efficacy and safety of IFX in the treatment of patients with BD-associated refractory posterior uveitis (PU).

The vascular manifestations of Behçet's disease: a case report

Behçet’s disease is an inflammatory vasculitis which affects the arteries and veins. The vascular pathologies are the rare complications of this disease. We present here a patient with Behçet’s disease who has been hospitalized several times because of plurifocal vascular manifestations.     

Pulmonary vasculitis in behcet disease: a cumulative analysis

STUDY OBJECTIVES: The aims of this study were to investigate the frequency of pulmonary problems in Beh?et disease (BD), and to discuss lesser-known features of pulmonary BD such as clinical characteristics, analysis of prognosis, and evaluation of treatment options with respect to the previously published cases. DESIGN: We conducted a comprehensive review of the literature to analyze cumulated data about pulmonary involvement in BD. SETTING: We found 159 articles regarding pulmonary disease associated with BD in May 2003. PATIENTS: The evaluation of these articles demonstrated 598 pulmonary problems in 585 cases. RESULTS: Pulmonary artery aneurysms (PAAs) are the most common pulmonary lesion in BD, and these are almost always associated with hemoptysis. Seventy-eight percent of patients with aneurysms have concomitant extrapulmonary venous thrombi or thrombophlebitis. Other pulmonary problems are reported in BD, and these are principally related to vascular lesions and radiologic abnormalities. CONCLUSIONS: Pulmonary vascular problems, either PAA or involvement of small-sized vessels, are the main pulmonary disorders in BD. Immunopathologic findings indicate that the underlying pathogenesis is pulmonary vasculitis, which may result in thrombosis, infarction, hemorrhage, and PAA formation. Patients with small nonspecific radiologic abnormalities should be followed up closely since early diagnosis of vascular lesions may be life-saving. Immunosuppression is the main therapy for the treatment of a vasculitis. It is important that pulmonary angiitis is not mistaken for pulmonary thromboembolic disease since fatalities have occurred in BD shortly after initiation of anticoagulation/thrombolytic treatment.