Inhibition of Erythroid Differentiation of Human Leukemia K562 Cells by N-acetylcysteine and Ascorbic Acid through Downregulation of ROS

正This study investigated the effects of N-acetylcysteine(NAC)and ascorbic acid(AA)on hemin-induced K562 cell erythroid differentiation and the role of reactive oxygen species(ROS)in this process.Hemin increased ROS levels in a concentration-dependent manner,whereas NAC and AA had opposite effects.Both NAC and AA eliminated transient increased ROS levels after hemin treatment,inhibited hemin-induced     

Grande précarité, psycho-traumatisme,souffrance narcissique : résultats d’une recherche-action à méthodologie quantitative et qualitative intégrée

In the context of a multi-annual research project, aiming at exploring the relations between traumatic biographic events since childhood and the structural functioning of personality at adult age, the results of a cross-sectional study with people living in great precariousness (n = 105) are discussed.     

Assessment of long non-coding RNA (THRIL and TMEVPG1) among Behçets' disease patients

BackgroundBehçet’s disease (BD) is a chronic inflammatory disorder with multifactorial cause. Long non-coding RNAs (lncRNAs) perform an essential role in gene regulation, and there is ongoing research in their contribution to autoimmune diseases.Aim of the workTo determine expression levels and diagnostic value of Theiler's Murine Encephalitis Virus Possible Gene1 (TMEVPG1) and TNFα and hnRNPL related immunoregulatory long non-coding RNA (THRIL) in BD, and to assess their role in the clinical characteristics of BD and disease activity.Patients and methodsStudy included 30 BD patients (12 females and 18 males) and 30 matched controls. Expression levels of TMEVPG1 and THRIL were detected by real-time polymerase chain reaction.ResultsThe mean age of patients was 37.5 ± 11.3 years and disease duration was 7.7 ± 6 years. Expression levels of TMEVPG1 and THRIL were upregulated significantly in serum of patients compared with controls (set as 1). TMEVPG1 fold change = 3.0 with interquartile range (0.2–546.9) (p < 0.0001), while THRIL fold change = 3.6 with interquartile range (0.1–112.3) (p < 0.0001). There was significant correlation between TMEVPG1 and THRIL (r = 0.48, p = 0.007). TMEVPG1 did not correlate with the clinical characteristics of patients, while THRIL correlated only with central nervous system manifestations. At a cut-off point of 1.3 the sensitivity of TMEVPG1 was 76.7%, while THRIL at 1.08 was 73.3% and specificity was 100% for both.ConclusionTMEVPG1 and THRIL were differentially expressed in the serum of BD patients and both had potential diagnostic value. Furthermore, an association between the expression level of THRIL and CNS symptoms was observed.     

纸基-表面增强拉曼光谱法快速鉴别染色南五味子药材

目的 采用纸基-表面增强拉曼光谱法(SERS)对染色南五味子进行快速鉴别。方法 选用浸泡法制备的银胶纸作为SERS基底,擦拭经乙醇-水溶液润湿的南五味子,银胶纸立即进行SERS检测;先后对银溶胶的浓缩倍数、银胶纸的SERS增强效果及稳定性等因素进行考察。结果 成功鉴别低浓度酸性红、赤藓红染色的南五味子。结论 纸基-SERS法可实现非法染色南五味子的快速、准确、无损的鉴别,有望应用于快检领域。     

铁皮石斛茶缓解小鼠体力疲劳作用的研究

目的研究铁皮石斛茶对小鼠的抗疲劳保健功能。方法给小鼠灌胃给药设低、中、高3个剂量组,同时设立对照组,观察小鼠负重游泳时间、肝糖原含量、血乳酸、尿素等指标的变化。结果铁皮石斛茶能明显延长小鼠负重游泳时间,显著增加小鼠肝糖原含量,减少小鼠运动前后血乳酸曲线下面积。结论铁皮石斛茶具有缓解体力疲劳功能。     

十批茵陈中药材的质量分析研究

目的：对10批茵陈的质量进行质量分析比较。方法：薄层色谱法对茵陈进行鉴别,高效液相色谱法测定茵陈主要成分绿原酸的含量。结果：10批茵陈按薄层色谱鉴别均为合格,绿原酸的含量分别为：甘肃0.62%;山东0.13%;宁夏1.10%;安徽0.72%;河北0.28%;四川0.60%;陕西1.50%;河南0.47%;湖南0.70%;山西1.40%。结论：宁夏、陕西、山西产茵陈质量最佳,山东、河北、河南产茵陈绿原酸含量达不到中国药典的标准,本试验结果可为生产厂家优选货源提供参考。     

Major flavonoid constituents and short-term effects of Chun Mee tea in rats

Chun Mee tea is a kind of green tea produced in China mainly for export purposes. Foam quantity is usually used as an index for evaluating the quality of Chun Mee tea. In the current study, we compared the concentrations of total saponin and flavonoids between foamy and low-foam Chun Mee tea. Our research confirmed that the total saponin and O-glycosylated flavonoid concentrations were related to the foam quantity of Chun Mee teas. We also studied the short-term safety effects of extract supplementation with foamy and low-foam Chun Mee tea in rats by routine blood tests and analysis of liver and kidney function, and blood lipids. Our results showed that both types of tea extract supplementations did not cause any observable adverse effects or impair either liver or kidney function. Additionally, this study confirmed the beneficial effects of Chun Mee tea extract supplementation on the decrease of total plasma cholesterol.     

Multiple brown tumor revealing primary hyperparathyroidism associated with Behçet’s disease: A case report

BackgroundPrimary hyperparathyroidism (PHP) is defined as hypercalcemia that may remain asymptomatic for years and cause lesion in skeletal system such as brown tumor (BT). Endocrine involvement in Behçet’s Disease (BD) regarding various systems can be seen.Case reportWe report an association between BD and PHP in a 45-year-old female. She had complained of inflammatory pain in the lower limb. Plain x-ray showed a lytic lesion on the upper tibia. Laboratory investigations revealed hypercalcemia (3.32 mmol/L), hypophosphatemia (0.48 mmol/L), a high parathyroid hormone (PTH) level (2278 pg/L) and a low vitamin D level (8.1 μg/L). An adenoma in the right lower parathyroid has been shown on scintigraphy, a vertebral fracture was present on lumbar spine X-ray and salt and pepper skull was shown on the lateral skull X-ray. Severe osteoporosis was present on the lumbar bone mineral density (t score ?3.3), and multiple BT were revealed on bone scan. The patient was operated on and developed Hungry Bone Syndrome (HBS) following surgery. Therefore, she received high doses of calcium with favorable outcome.ConclusionThis case is a leading report for the coexistence of BD with PHP. This case provides further evidence that BD should be considered as an autoimmune process, which may be associated with endocrine involvement including PHP. On the other hand, musculoskeletal manifestations of PHP including BT still seen in daily practice require continued vigilance on the part of the physician.     

附子大黄配伍对阳虚便秘动物的治疗作用及其机制研究

[目的]探讨附子大黄配伍对阳虚便秘动物的治疗作用及其机制。[方法]观察附子大黄不同配伍比例对阳虚便秘小鼠、大鼠的排便时间、排便量、小肠推进作用的影响；对阳虚便秘大鼠血中胃动素（MOT）、促胃液素（Gas）、内皮素（ET）、生长抑素（Ss）、P物质（SP）及肠神经递质血管活性肠肽（VIP）、乙酰胆碱酯酶（AchE）的影响。[结果]与模型组比较，附子大黄配伍组动物的首粒排便时间显著缩短，3h内排便粒数显著增加，小肠推进率明显提高，其疗效优于附子单煎剂或大黄单煎剂；附子大黄配伍组动物血中MOT、Gas、ET、AchE显著升高。[结论]附子大黄配伍对阳虚便秘动物具有确切的治疗作用，其作用机制可能与其干预胃肠激素及肠神经递质的分泌有关。