Effect of maternal aseptic inflammation on the structural organization of extraembryonal organs and on the proliferative activity of embryonic liver and lung cells in rats

After 2 days of aseptic inflammation in pregnant rats the number of hematopoietic lineage cells in the embryonic liver decreased, while proliferative activity of cells in the embryonic lung increased. Degenerative changes were noted in the placenta. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 5, pp. 584–587, May, 1997     

回转器旋转对鸡胚脑细胞内游离Ca^2＋水平的影响及其恢复

探讨了不同胚龄鸡胚旋转不同时间后，脑细胞内游离Ｃａ＾２＋水平的变化，利用回转器模拟微重力生物效应及ｆｕｒａ－２比例荧光法，测定了孵化第６至１８ｄ鸡胚脑细胞游离Ｃａ＾２＋的浓度。结果显示：孵化第８至１７ｄ鸡胚经旋转不同时间后，其脑细胞内游离Ｃａ＾２＋浓度均有所下降其中１０ｄ鸡胚旋转４或７ｈ及第１３ｄ鸡胚旋转２４ｈ后，脑细胞内Ｃａ＾２＋浓度比对照组显著降低（Ｐ〈０．０１），第１０ｄ和１３ｄ鸡胚分别旋转     

Needs and use of drug information sources in community pharmacies: a questionnaire based survey in German-speaking Switzerland

OBJECTIVES: To explore the types of drug information used by pharmacists in daily practice, their needs and wishes concerning drug information and their opinions about future changes of pharmacy practice. METHODS: Postal survey to a random sample of 223 (26% out of 859) community pharmacies from the German-speaking part of Switzerland. The 48-item questionnaires were processed automatically with the Cardiff TELEform-Software. In addition, a telephone-survey to a random sample of 20 non-responders was performed in order to test for non-response bias. RESULTS: A total of 108 pharmacists (response rate 48%) reported that the official Swiss drug reference book is still the most popular source of drug information used to solve all kinds of drug related problems. The Internet as a source of drug information is of minor importance, even though 88% of the pharmacies have Internet access. Deficits in drug information were reported for paediatrics, phytotherapy, drugs during pregnancy/lactation and for therapy guidelines. According to 35% of the pharmacists, the importance of offering drug information to customers will increase in the future. Most of the pharmacists are not afraid that Internet pharmacies would replace them. CONCLUSIONS: The results show that the majority of the community pharmacists are only partially satisfied with the sources of drug information currently available. The Internet still plays a minor role for solving drug-related problems in daily practice, even though the available infrastructure makes the community pharmacies able to use the Internet more frequently. The pharmacists need more websites tailored to their needs. The pharmacists have clear visions about possible future developments. They do, however, have to adopt quickly to the changes ahead in order to remain competitive.     

小鼠胚胎单个心肌细胞自发节律性活动的发育依赖性变化

目的　研究小鼠胚胎心房肌和心室肌细胞自律性水平随胚胎发育不同时期而改变的发育依赖性特点。方法采用全细胞膜片钳技术 ,在电流钳条件下 ,记录了不同胚胎发育阶段小鼠单个心室肌细胞和心房肌细胞的跨膜动作电位。结果　心室肌细胞早期 (9 5～ 10 5d ,earlydevelopmentstage,EDS)、中期 (11～ 14d ,intermediatedevelopmentstage ,IDS)、晚期 (14～ 18 5d ,latedevelopmentstage,LDS)动作电位周期分别为 (172 86± 2 6 15 )ms、(2 4 7 73±15 84 )ms、 (32 6 6 7± 30 10 )ms (P <0 0 5 ) ;中、晚期心房肌细胞动作电位周期分别为 (2 0 4 15± 2 4 14 )ms,(2 2 6 5 3± 2 0 2 8)ms。结论　随着胚胎发育成熟 ,小鼠胚胎心房肌和心室肌细胞的自律性活动逐渐降低。     

敲除PKD1基因的小鼠胚胎干细胞的建立

目的：通过PKD1基因打靶载体的胚胎干细胞转染，药物筛选以及分子鉴定，获得发生同源重组的胚胎干细胞克隆，为产生PKD1基因缺陷小鼠品系准备条件。方法：体外培养小鼠胚胎干细胞，用电穿孔的方法进行PKD1基因打靶载体的转移，并用G418进行筛选培养，得到阳性克隆后，进一步扩大培养，抽提胚胎干细胞的基因组DNA，采用DNA印迹法进行分子鉴定。结果：经G418筛培养得到192个阳性在隆，分子鉴定获得2个发生同源重组的胚胎干细胞克隆。结论：鉴定得到的2个同源重组胚胎干细胞克隆，为进一步建立PKD1基因缺陷小鼠奠定了基础。     

Sensitive three-parameter flow-cytometric detection of abnormal cells in human cervical cancers: A pilot study

Summary The cell volume, the DNA, and the carcino-embryonic (CEA) or epithelial-membrane (EMA) antigen of formaldehyde-fixed human cervical cells from 21 malignant cervix tumors and 11 normal patients were measured simultaneously with a Fluvo-Metricell flow cytometer. The simulataneous cell volume and DNA measurement provided the distinction of morphologically intact cells from cell debris, the determination of the cell cycle phase combined with the detection of aneuploid cells, and the distiction of inflammatory cells from parenchymal and tumor cells. Malignant samples were recognized because they contained more than 0.5% CEA positive cells which were of intermediate size. CEA and EMA expression in the malignant samples was not linked. The false positive rate in a total of 32 samples was 6.3% when the sum of CEA and EMA positive cells of each cell sample was calculated. No false negative malignant sample was observed.