795例肠外营养支持的回顾性分析

目的:对795例患者的肠外营养支持进行回顾性分析.方法:对行肠外营养支持的399例患者进行治疗前后淋巴细胞计数、血浆白蛋白和血浆锌离子浓度等营养学指标的比较,并选择行肠外营养的患者198例与行单一静脉营养的患者198例进行营养学指标的比较,同时比较不良反应和依从性.另选择25例有单一静脉营养史患者25例,分为足量组13例和低氮组12例,分别按照1.0～1.5和0.6 g·kg-1·d-1逐渐在3 d内递增到1.0～1.5 g·kg-1·d-1给予氨基酸,其余营养支持相同, 进行营养学指标的比较.结果:与治疗前比较,行肠外营养支持后患者的白蛋白、淋巴细胞计数、锌离子浓度等均显著增加(P<0.05).与单一静脉营养相比较,行肠外营养患者的白蛋白、淋巴细胞计数、锌离子浓度等也均显著增加(P<0.05),同时不良反应和依从性较好.足量组与低氮组比较,白蛋白值显著增加,而淋巴细胞计数、锌离子浓度无显著性差异.结论:肠外营养支持可以显著改善营养不良.     

酮洛芬的相转移催化氧化合成

2-(3-苄基苯基)丙醛和高锰酸钾在相转移催化剂度米芬作用下,以苯为有机相、氢氧化钠水溶液为水相氧化制得酮洛芬,收率69%,纯度99.5%.     

单槽单桨磷酸萃取槽

讨论湿法磷酸采用单槽单桨萃取槽的工程投资 ,并与单槽多桨对比其经济效益     

不同载体介导的绿色荧光蛋白基因在大鼠颌下淋巴结的表达

目的探讨阳离子聚合物Sofast基因转染试剂(Sofast)和阳离子脂质体Lipofectamine2000(Lipo)经不同途径向大鼠转入绿色荧光蛋白基因(PEGFP-N2),观察其在颌下淋巴结(SMLN)的表达。方法45只Wistar大鼠随机分成9组:Lipo/DNA前房注射组(A)、结膜下注射组(B)、颞下方穹隆部注射组(C)和下睑皮下注射组(D);Sofast/DNA前房注射组(E)、结膜下注射组(F)、颞下方穹隆部注射组(G)、下睑皮下注射组(H)和阴性对照组(I)。分别于注射后48小时取同侧SMLN,通过荧光显微镜观查和流式细胞仪(FCM)检测各组的SMLN中PEG-FP-N2的表达。结果A-D、E-H各实验组的SMLN中均可观察到绿色荧光。相同载体不同部位注射DNA后颞下方穹隆部注射组和下睑皮下注射组可以获得相对较高的转染效率。相同部位不同载体注射DNA后Sofast/DNA注射组的转染效率高于Lipo/DNA注射组。结论Sofast在SMLN的基因转染中优于Lipo。DNA/Sofast可以通过颞下方穹隆部注射和下睑皮下注射在SMLN获得相对较高的表达。     

CCL2-2518λ������̬������֢��������71�͸�Ⱦ������о�

??Objective To investigate the relationship between CCL2-2518A/G gene polymorphism and severity of enterovirus 71??EV71?? infection. Methods CCL2 gene polymorphism in 188 EV71-infected patients and 235 healthy controls were detected by the improved multiplex ligation detection reaction technique??iMLDR??. The level of CCL2 in two groups was determined by enzyme-linked immunosorbent assay??ELISA??. Results No significant differences were found in the distribution of genotype CCL2-2518A/G between EV71-infected patients and the healthy control group??P??0.05??. The G allele??genotypes AG or GG?? in the CCL2-2518A/G??P??0.001?? was more frequent in patients with severe EV71 infection. The level of CCL2 in infected patients was higher than that of the heathy controls ??P??0.05???? the severe cases had higher level of CCL2 than that of the slight cases and healthy controls??P??0.05??. The level of CCL2 in GG gene group was significantly higher than that in AG gene group and the level of CCL2 in AG gene group was significantly higher than that in AA gene group.The people with CCL2-2518G allele??GG+AG?? had higher level of CCL2 than those only with CCL2-2518A allele??AA????P??0.05??. Conclusion The G carrier of the CCL2-2518A/G is found to be associated with severity of EV71 infection??and could be susceptibility factors in the development of EV71 infection.     

Fertilization of supernumerary oocytes following gamete intrafallopian transfer (GIFT): Correlation with outcome of GIFT treatment

In the course of 280 consecutive gamete intrafallopian transfer (GIFT) attempts, supernumerary oocytes in excess of those transferred were inseminated in vitro. Pregnancy resulted in 31.1% of patients following the GIFT treatment. The overall in vitro fertilization (IVF) rate of supernumerary oocytes was significantly higher in those who became pregnant (41.2%) than in patients who did not (34.9%). However, the failure to fertilize any supernumerary oocytes was not significantly different between those becoming pregnant (27.6%) and those in whom the treatment failed (37.3%). Failure to fertilize any supernumerary oocytes in vitro was of a higher rate if suboptimal sperm preparation was used or if only one oocyte was left over for in vitro insemination following the GIFT treatment. We concluded that the in vitro fertilization rate was higher in the pregnant group, but the total failure to fertilize or the fertilization of any supernumerary oocytes does not predict the outcome of GIFT. Embryos resulting following fertilization of excess oocytes may be cryopreserved for subsequent use.     

不同发育天数囊胚冻融移植后妊娠结局分析

目的比较不同发育天数冻融囊胚移植后的妊娠结局。方法回顾性分析1 176例行冻融囊胚移植患者的临床资料,其中植入前遗传学诊断(preimplantation genetic diagnosis,PGD)周期135例,比较第5日单囊胚移植(single embryo transfer,SET)组、双囊胚移植(double embryo transfer,DET)组与第6日SET组和DET组的妊娠结局。结果同是优质囊胚级别的条件下,第5日DET组的生化妊娠率(56.91%)、临床妊娠率(53.25%)以及多胎妊娠率(1.20%)显著高于其他组(P0.05);第5日DET组与SET组的胚胎着床率无统计学差异(P0.05),但均显著高于第6日DET组和SET组(P0.05);同级别以及PGD周期中第5日和第6日SET组相比较,第5日组的生化妊娠率、胚胎着床率均显著高于第6日组(P0.05)。结论在同是优质囊胚的条件下,第5日SET、DET及经PGD诊断的SET妊娠结局均优于第6日。     

复方转移因子激发CD4^＋T淋巴细胞的实验研究

目的用化疗药物建立免疫细胞抑制的动物模型，以免疫细胞为观察指标评价复方转移因子的提高免疫细胞的能力。方法用环磷酰胺（CTX）灌胃（100mg／kg），隔天一次，连续五次，造成免疫功能抑制的动物模型，通过不同实验组用药前后免疫细胞的变化，以评价复方转移因子高、中、低（相当人用量的2、5、10倍）剂量组是否能提高实验动物的免疫细胞。结果复方转移因子能显著提高实验小鼠白细胞数量，复方转移因子低、中、高剂量组与模型组比较，P〈0．01，差别有极显著性；复方转移因子能显著提高实验小鼠淋巴细胞百分比及CD4^＋T淋巴细胞的绝对数，P〈0．01，差别有极显著性。结论复方转移因子能提高实验小鼠WBC及CD4^＋T淋巴细胞的数量，并与剂量有关。