代红雨治疗臁疮经验

代红雨教授认为,臁疮患者全身证候与其创面局部表现并非全都处于相同阶段,应整体辨证、局部辨证相结合,内治法侧重于"补虚""化瘀",外治法侧重于拔毒、调节炎症反应.臁疮的本"虚"在于肝肾所主之"筋"的先天功能不足,因此,内治法尤重补肝肾、强筋力.臁疮创周的改变与微循环障碍、慢性炎症反应有关,创周与创面的状态有很大相关性,因...     

Ecthyma gangrenosum occurring at sites of latrogenic trauma in pediatric oncology patients

We report two cases of ecthyma gangrenosum which occurred at sites of iatrogenic trauma. The first case developed due to metastatic seeding with Pseudomonas aeruginosa during an episode of septicaemia and the second case occurred as a primary skin lesion. Both required prolonged courses of antibiotics and one patient died. The different pathogenic mechanisms and outcomes associated with this condition are discussed. © 1996 Wiley-Liss, Inc.     

全国名老中医崔公让治疗臁疮经验

目的：探讨全国名老中医崔公让教授治疗臁疮的临床经验。方法：分析崔公让教授临床对臁疮病因病机的认识以及专方论治方法。结果：崔公让教授认为臁疮的病机以＂虚＂为本,关键在于＂湿＂和＂瘀＂。强调在中医内治的同时应重视外治疗法,自拟赤芍甘草汤和疮疡外洗方用于临床。结论：崔公让教授临床治疗臁疮用药精当,疗效佳。     

Molecular diagnostics of parapox virus infections

Background: The diagnosis of parapox virus infections relies primarily on a history of contact with infected animals. The clinical presentation is usually a non‐specific necrotic ulcer. The histology may also be non‐specific, especially with older lesions. Negative‐staining electron microscopy (EM) is a fast and reliable diagnostic tool, but is not widely available. Serological tests and the time‐consuming viral culture are also rarely used in Europe. Patients and methods: The diagnostic procedure in two patients with ecthyma contagiosum and milker's nodule using polymerase chain reaction specific for orthopox, parapox and Orf virus is explained. Diagnostics included bacterial culture, viral culture, histology and EM. In addition to these, a polymerase chain reaction (PCR) was performed in both cases. Results: The patient with ecthyma contagiosum was negative for ortho‐, parapox‐, and orf‐virus on PCR, whereas the patient with milker's nodule had a PCR positive for parapoxvirus. Conclusions: PCR is a simple, fast, and standardized method of diagnosis that can distinguish between the subgroups of parapoxviruses. A diagnosis can be made even in cases of ambiguous history or unspecific clinical presentation. The method is limited by the necessity to sample native material or to use neutrally buffered formalin in case of PCR from paraffin material.     

Ecthyma gangrenosum in an infant after liver transplantation

Ecthyma gangrenosum is a rare, necrotizing, bacterial infection of the skin most commonly caused by Pseudomonas aeruginosa. It has a characteristic clinical picture starting with maculopapular eruption followed by hemorrhagic vesicle and evolving into gangrenous ulcer. Although direct skin inoculation without septicemia is also probable, usually ecthyma gangrenosum is pathognomonic for Pseudomonas septicemia, which has a mortality rate of 38–96%. Herein, we report on the case of a 9‐month‐old male infant diagnosed with ecthyma gangrenosum who had undergone liver transplantation approximately 6 months previously and who was under immunosuppressive treatment, in order to highlight the importance of the early diagnosis and treatment of this disease.     

Pasteurella multocida infection in a post-chemotherapy neutropenic host following cat exposure

We report a case of Pasteurella multocida infection in a neutropenic patient. History of contact with animals is important, particularly in patients who present with localized cellulitis and neutropenic fever. Clinical features and treatment of the disease are discussed. Copyright 2002 The Royal College of Radiologists. Published by Elsevier Science Ltd. All rights reserved.     

Virological and clinico-pathological features of orf virus infection in experimentally infected rabbits and mice

Many aspects of the biology of orf virus (ORFV) infection remain poorly understood and attempts to establish animal models have yielded conflicting and non-reproducible results. We herein describe the characterization of ORFV infection and disease in rabbits and mice. A protocol of intradermal inoculation was employed to inoculate 10^8.5TCID₅₀/mL of ORFV strain IA-82 in the skin of ears, of the back and labial commissures. All inoculated rabbits presented a clinical course characterized by erythema, macules, papules/vesicles or pustules that eventually dried originating scabs. Local signs started around days 3 and 4 post-inoculation (pi) and lasted 3–10 days. Virus was recovered from lesions between days 2 and 14pi. Histological examination of lesions revealed focal proliferative dermatitis with ballooning degeneration and eosinophilic intracytoplasmic inclusion bodies in keratinocytes, histological hallmarks of contagious ecthyma in sheep. A similar, albeit milder clinical course occurred in 5/10 inoculated mice; virus was recovered from lesions from three animals. Inoculated lambs – used as controls – developed severe lesions of contagious ecthyma. VN tests performed at day 28pi failed to detect neutralizing antibodies in all inoculated animals. In contrast, convalescent rabbit sera were positive by ELISA at dilutions from 100 to 400. These results show that rabbits are susceptible to ORFV infection and thus may be used to study selected aspects of ORFV biology.     

Orf virus infection

Orf virus (ORFV) is an important pathogen responsible for a highly contagious zoonotic viral infection that threatens those who handle sheep and goats. Orf virus is the prototype of the Parapoxvirus genus, and its resilience in the environment and ability to reinfect its host has contributed to the spread and maintenance of the infection in many species. In healthy humans, the disease usually resolves spontaneously within 3 to 6 weeks. There is no specific treatment and many different approaches such as use of imiquimod, cidofovir, curettage, shave excision, cryotherapy, and electrocautery have all been reported to be successful, without supporting evidence from controlled clinical trials. Throughout its interaction with the different hosts, ORFV has evolved a strategy for immune evasion via the development of an array of virulence factors. The interaction of ORFV with the immune system has been the subject of research for decades. Whole inactivated ORFV has been used as a type of immunomodulating drug; a so called paramunity inducer proposed as both a preventative and a therapeutic immunomodulator across various species. Additional research on the remarkable strategies underlying ORFV infection could lead to improved understanding of skin immunity.     

中医治疗联合介入手术治疗下肢深静脉血栓形成后综合征的临床疗效

目的:观察中医治疗联合介入手术治疗下肢深静脉血栓形成后综合征的临床疗效。方法:回顾性分析2016 年1月—2019 年1 月我院收治的下肢深静脉血栓形成后综合征患者60 例,其中治疗组30 例患者应用中医治疗+ 介入手术,对照组30 例应用非中医治疗+ 介入手术。结果:术后3 个月,治疗组患者创面痊愈率为86.7%,大于对照组的60%,差异有统计学意义(P <0.05),治疗组总有效率为96.7%,与对照组的90% 差异无统计学意义(P >0.05)。治疗组患者在治疗后3、12 个月的Villalta 评分均低于对照组(P <0.05) ;治疗组3 个月时血管通畅率为93.3%,与对照组的90% 差异无统计学意义(P >0.05),治疗组12 个月的血管通畅率为80%,优于对照组的55.2%,差异有统计学意义(P <0.05)。治疗组的创面愈合时间为40(15)天,明显短于对照组的55(35)天,差异有统计学意义(χ2=7.215,P =0.007)。结论:中医治疗联合介入手术治疗下肢深静脉血栓形成后综合征安全有效。