干漆及大黄䗪虫丸中非法染色成分808猩红的检测

目的 建立干漆及大黄?虫丸中非法染色成分808猩红的检测方法.方法 样品经三氯甲烷提取氮吹浓缩后甲醇复溶液的分析采用Boston C18色谱柱(4.6 mm × 250 mm,5 μm);流动相乙腈-0.1％甲酸;检测波长518 nmn.液质联用确证采用Waters HSS C18色谱柱(2.1 mm × 100 mm...     

Direct Measurement of ATP7B Peptides Is Highly Effective in the Diagnosis of Wilson Disease

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葡萄冻干粉在线粒体应激介导肝细胞凋亡中的作用

目的 了解葡萄冻干粉（FDG）对线粒体应激介导的肝细胞凋亡的阻抑作用,探讨其治疗肝细胞损伤的作用机制。方法用牛磺酸脱氧胆酸（TDCA）诱导Huh7细胞,建立线粒体应激凋亡模型,用不同剂量FDG进行干预。通过MTT、DNA ladder、Western blot、细胞凋亡率检测等方法,了解FDG对Huh7细胞生长、对TDCA诱导的Huh7细胞的凋亡率、凋亡信号蛋白表达的影响。结果FDG（2μg／ml、20μg／ml、200μg／ml、4（10μg／ml和800μg／ml）与Huh7细胞共孵育,FDG可提高Huh7细胞的活力,以FDG400μg／ml时细胞活力最高。用400pMTDCA诱导Huh7细胞,随着诱导时间延长细胞活力下降、凋亡率增加,出现凋亡条带;Caspase-9和7蛋白酶原被活化,PCNA表达下调。用FDG干预TDCA诱导Huh7细胞48h后发现,FDG明显提高Huh7细胞活力、使细胞凋亡减少、凋亡信号蛋白的表达减少。结论TDCA能触发Huh7细胞线粒体氧化应激凋亡,而FDG能促进细胞生长,减轻肝细胞损伤,阻断线粒体氧化应激介导的肝细胞凋亡。     

In Vitro Direct and Indirect Cytotoxicity Comparative Analysis of One Pre-Hydrated versus One Dried Acellular Porcine Dermal Matrix

Aim: The aim of the present study was to compare the direct and indirect cytotoxicity of a porcine dried acellular dermal matrix (PDADM) versus a porcine hydrated acellular dermal matrix (PHADM) in vitro. Both are used for periodontal and peri-implant soft tissue regeneration. Materials and methods: Two standard direct cytotoxicity tests—namely, the Trypan exclusion method (TEM) and the reagent WST-1 test (4-3-[4-iodophenyl]-2-[4-nitrophenyl]-2H-[5-tetrazolio]-1,3-benzol-desulphonated)—were performed using human primary mesenchymal stem cells (HPMSCs) seeded directly onto a PDADM and PHADM after seven days. Two standard indirect cytotoxicity tests—namely, lactate dehydrogenase (LTT) and MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazoliumbromide)—were performed using HPMSCs cultivated in eluates from the matrices incubated for 0.16 h (10 min), 1 h, and 24 h in a serum-free cell culture medium. Results: The WST and the TEM tests revealed significantly lower direct cytotoxicity values of HPMSCs on the PHADM compared with the PDADM. The indirect cytotoxicity levels were low for both the PHADM and PDADM, peaking in short-term eluates and decreasing with longer incubation times. However, they were lower for the PHADM with a statistically significant difference (p < 0.005). Conclusions: The results of the current study demonstrated a different biologic behavior between the PHADM and the PDADM, with the hydrated form showing a lower direct and indirect cytotoxicity.     

Mixing is required for uniform reconstitution of filter-dried protein antigens in a single-injection vaccine formulation

Ambient temperature filter dried vaccine formulations have been proposed to simultaneously achieve thermostability and offer a ready-to-use immunisation device that combines reconstitution and injection. Vaccine concentration should be uniform at the point of injection, but the uniformity following direct reconstitution of filter-dried vaccines has not been reported. We present here a study of vaccine mixing and release following dissolution of filter-dried model protein and toxoid antigens within a single syringe, filter and needle unit. Release was better for filters made from glass than cellulose. Without additional mixing, uniformity was poor and only 41% of input protein was released from protein filter-dried onto glass fiber. In contrast, adding a simple glass bead and mixing by inversion, 100% release antigen solution was achieved, with uniform concentration at exit from the needle throughout a simulated injection. Adsorption onto alum adjuvant had no detectable effect on vaccine dissolution and mixing. The uniformity and yield of low doses of diphtheria and tetanus toxoid was also improved by mixing, albeit with a lower yield of 60–68%. We conclude that uniformity and mixing should be studied to ensure safety and efficacy of directly reconstituted filter-dried vaccine formulations.     

紫河车为主治疗呼吸系统疾病三则

紫河车可用于各类虚损性病证的治疗,其中在呼吸系统疾病中的应用最为广泛。紫河车对肺病日久所致的肺肾两虚、肾不纳气具有明显的疗效,远优于植物类补益药。     

蛇床子总黄酮对去卵巢骨质疏松大鼠股骨骨密度及生物力学的影响

目的探讨蛇床子总黄酮对去卵巢（OVX）骨质疏松大鼠股骨骨密度及股骨生物力学的影响。方法40只6、7月龄雌性SD大鼠随机分为4组：假手术（SHAM）组,去卵巢（OVX）组,OVX＋已烯雌酚（E）组和OVX＋蛇床子总黄酮组,每组10只。实行手术后,于术后第三天开始灌胃用药。90天后处死大鼠,取左测股骨进行股骨骨密度和股骨生物力学的测量。结果去卵巢后股骨骨密度及股骨生物力学指标明显下降;用蛇床子总黄酮后,股骨骨密度及股骨生物力学指标均增高。结论蛇床子总黄酮对去卵巢（OVX）造成的骨质疏松有一定的防治作用,能防止骨生物力学性能的受损,效果略逊色于已烯雌酚。     

工艺因素对冻干骨浸泡掺锶的影响

摘要 背景：冻干骨是临床上常用的自体骨替代材料,在冻干骨中掺入成骨活性元素锶将有助于冻干骨在临床上发挥更大的作用。 目的：观察含锶溶液浓度和处理时间对冻干骨浸泡掺锶的影响,并对锶离子的缓释特征进行观察。 方法：采用5~40 mmol/L的氯化锶溶液处理冻干骨,并对其中浓度10 mmol/L组分别在浸泡1.5,3,6,9 d进行检测,观察在不同时间内锶元素的掺入效率;在去离子水中进行锶离子的缓释效果评价。用ICP-OES等离子体发射光谱仪对元素含量进行分析。 结果与结论：随溶液浓度升高锶元素的掺入量增加,浓度在2.0%~5.1%范围。在所观察的9 d时间内,锶掺入浓度随浸泡时间延长持续增加。锶元素在初期存在爆释效应,之后进入较平稳的释放阶段。30 d内锶离子释放量始终高于松质骨中的钙离子释放量。提示在实验观察的范围内,采用较高含锶浓度溶液以及延长浸泡时间可以提高锶元素在冻干骨中的掺入量。掺锶冻干骨具有长期释放锶离子的能力。