Human gastric lipase: ontogeny and variations in children

The aim of this study was to investigate the precise origin of the acid pre-duodenal lipase during human development and to evaluate its possible changes, at the tissue level, in children with gastritis or pancreatic insufficiency. Human gastric lipase appears around the 11th week of gestation and increases slowly during pre- and postnatal development. It is localized in the fundus of the stomach without any lingual localization. Human gastric lipase reaches its adult level in the third month of life, and does not vary in relation to pancreatic insufficiency. It is only rarely impaired during gastritis.     

Location of the three disulfide bonds in an antimicrobial peptide from Amaranthus caudatus using mass spectrometry

The disulfide bridge pairing of Ac-AMP2, a 30-residue peptide isolated from the seeds of Amaranthus caudatus, is determined using a fast method involving enzymatic fragmentation followed by identification of the fragments with FAB mass spectrometry. The results confirm the location of the three disulfide bridges as previously established by NMR spectroscopy and molecular modelling. © Munksgaard 1997.     

Saliva: The misunderstood and underrated fluid

This review begins with a brief consideration of the many roles of saliva. Then a summary is given of the eight studies by our laboratory on the physiological and psychological relationships of stress, relaxation and saliva. This is followed by a consideration of the effects of stress and relaxation on dental caries. Finally, a summary is provided of the two studies by our laboratory on the relationship of stress and relaxation to oral digestion of complex carbohydrates.     

Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line

We have used a restriction endonuclease/nick translation (RE/NT) procedure to study the ability of restriction enzymes to cleave DNA in fixed chromosomes of a fish cell line. This technique has proved to be very useful in revealing the chromatin heterogeneity underlying the chromosome structure that remains cryptic to other techniques also able to induce longitudinal differentation on fish chromosomes. The differences observed in the banding patterns after nick translation procedure seem to be due, at least in part, to differences in activity among the enzymes assayed. The results obtained also reveal some evidence about the origin and evolution of the marker chromosomes of the Atlantic salmon cell line.     

微波消解-原子吸收光谱法测定头发中多种金属元素

目的：探索并建立快速检测头发中铁、锰、铜、锌、钙、铅、镉、硒含量的方法。方法：微波消解头发样,火焰原子吸收法测定铁、锰、铜、锌,石墨炉原子吸收法测定铅、镉,氢化物发生原子吸收法测定硒。结果：测定值均在标准物质标准值不确定度范围内,相对标准偏差均小于8%。结论：本法准确度和精密度令人满意,适于头发中多种金属元素的测定。     

微波消解-无色散原子荧光法直接测定食品添加剂中镉

目的：建立氢化物发生无色散原子荧光法测定食品添加剂中镉的方法。方法：样品在光纤压力自控密闭微波消解系统中消解，消解采用阶梯式升压方式，分别为0．5MPa 1min、1．0MPa 2min、2．0MPa 2min消解样品，以Fe^3＋、KMnO4、EDTA、硫脲作掩蔽剂。结果：方法相对标准偏差（RSD）小于5％。加标回收率95．0％-103．0％。结论：用该法测定食品添加剂中的镉，结果满意。     

减肥药物最新研究进展

减肥药物按作用机制可分为3类：抑制食欲药物、抑制肠道消化吸收药物和增加能量消耗药物。现分别介绍上述各类中的代表性药物和正处于临床或临床前研究阶段、有开发前景的药物的最新研究进展。     

9厂家牛黄解毒片中5种微量元素检测

目的:测定牛黄解毒片中铬(Cr)、锰(Mn)、铁(Fe)、锌(Zn)、铜(Cu)5种微量元素的含量,以对其质量进行监控。方法:采用压力溶弹消解快速火焰原子吸收法对牛黄解毒片中的Cr、Mn、Fe、Zn、Cu5种微量元素含量进行测定。结果:9个不同厂家生产的牛黄解毒片中Cr、Mn、Zn、Cu含量相差不大,而Fe含量则有较大差异,且远高于其它元素的含量。结论:该方法简便快速、准确可靠,是一种适合本品常规分析的检测方法;本品不同品牌间各元素的含量差异很大,可能导致疗效有所差别。     

湿法消化-氢化物原子荧光法测定豆类食品中汞的含量

目的:探讨应用AFS-930型双道原子荧光光度计测定豆类食品中汞含量的方法和技术。方法:以5%的盐酸为介质,15 g/L硼氢化钠为还原剂,AFS-930原子荧光分光光度计测定。结果:该法线性范围为0.0~10.0μg/L,相关系数r=0.9998,检出限为0.0494μg/ml,相对标准偏差为1.17%,回收率为93.6%~104.0%。结论:该方法精密度好,回收率高,操作简单,是测定豆类食品中汞含量的可靠方法。